Regalin Rout

Aurobindonagar, Madhupatna

Cuttack-753010, Orissa

E-mail: acajkv@r.postjobfree.com

Ph: +91-943*******(M), +91-671-*******

CAREER OBJECTIVE:

Seeking a management position in the area of Life Sciences/Molecular Biology to enhance my

research skills and scientific outlook in order to build an independent career in science.

Experience working in teams of international researchers.

EDUCATION:

Ph.D in Biotechnology (2012). School of Biotechnology, KIIT University, Bhubaneswar, Odisha

Scope of work:

• Isolation and characterization of indigenous mosquitocidal strains of Bacillus

thuringiensis.

• Bioactivity studies against different insect vectors such as Aedes, Anopheles and Culex

and evaluated for commercial application.

• Molecular investigation on delta-endotoxins of novel Bacillus thuringiensis israelensis

strains to be used to control mosquito larvae as vectors of infectious human diseases. This

includes sequencing of cry genes and identification of Cry proteins by MALDI-TOF.

Supervised by Dr. Vishakha Raina and Co-supervised by Prof. Dr. P. Luethy.

PGDBM in HR, Symbiosis Centre for Distance Learning(SCDL), Pune. 2011

Masters in Microbiology (2006). Orissa University of Agriculture and Technology,

Bhubaneswar, Odisha.

Bachelor in Science (Zoology (Hons) with Distinction; 2004), Utkal University, Bhubaneswar,

Odisha.

Domain Expertise:

Microbiology:

Bacterial stock maintenance and media preparation.

Isolation, identification and characterization of bacteria by microbiological, biochemical

and molecular approaches.

Molecular Biology:

Isolation of DNA, RNA, total meta-genomic DNA from soils and sediments.

Genomic and plasmid DNA extraction from prokaryotes.

Cloning and sequencing in prokaryotic system.

Polymerase chain reaction(PCR), RT PCR.

Agarose Gel Electrophoresis, Gel Documentation, RFLP.

Protein chemistry:

Isolation and purification of proteins samples from bacteria.

SDS-PAGE, NATIVE-PAGE.

Identification of proteins by MALDI-TOF analysis and purification of Crystal proteins

by density gradient centrifugation.

Scientific computer software:

Phylogenetic analysis (PHYLIP, MEGA 5.0, EzTaxon), ClustalW for multiple

sequence alignment.

Bionumerics for editing and assembling of nucleotide sequences, EndnoteX2 for

bibliography. For statistical analysis GraphPad Prism5 and for Probit Analysis Biostat

2009.

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Knowledge on Functional Genomics and Intellectual Property Rights (IPR).

Computer proficiency:

General application (MS Windows, MS Office, Mac OSX etc.)

Information Technology and Programming in Computer Application.

OVERSEAS RESEARCH EXPERIENCE:

Worked for 4 months at “Cantonale Institute of Microbiology” (ICM), Mirasole 22A, 6500

Bellinzona, Switzerland under the supervision of PD Dr. Mauro Tonolla, Faculty of Science,

University of Geneva, Switzerland from 11th August 2011-4th December 2011.

This includes molecular typing of Bacillus thuringiensis israelensis (Bti) isolates, toxicity assays

on mosquito larvae as well as proteomic analysis of Crystal toxins. In the mean time involved in

an ongoing Bti project at ICM which is on “Fate of Bacillus thuringiensis var. israelensis (Bti)

applied as a biocontrol agent against mosquito larvae in the wetland reserve "Bolle di Magadini".

Worked also with Dr. Pie Mueller, Swiss tropical Institute, Basel, Switzerland.

TEACHING EXPERIENCE:

• Faculty in Biotechnology, Trident Academy of Creative Technology, Affiliated to Utkal

University, Bhubaneswar, Odisha. 2007.

• Conducted training workshops of MSc, MTech students at ENOVEO, TBI, KIIT

University.

PUBLICATIONS:

1. Rout, R., V. Raina, M. Suar, and P. Luethy. 2011. High prevalence of bacterial spore-

formers active against mosquito larvae in temporary monsoon flooded sites in Orissa,

India. J Am Mosq Control Assoc 27(2): 159–161. (Published)

2. Rout, R., V. Raina, M. Suar, and P. Luethy. A novel mosquitocidal bacterial strain

Bacillus thuringiensis odishaensis sp. nov. isolated from road side soil in Bhubaneswar,

India. (Manuscript under preparation)

3. Rout, R., V. Raina, M. Suar, M. Tonolla, S. de Respinis, N. Storelli, V. Guidi and P.

Luethy. Evaluation of efficacy of the two potential B. thuringiensis isolates for control of

vector-borne diseases. (Manuscript under preparation)

AWARDS:

Best Poster Award winner in the 49th Annual AMI (Association of Microbiologists in India)

Conference held in New Delhi. 2008

Best Write-up award winner in the training workshop “Research Communication for Societal

Change” co-convened by the Centre for the Environment and Public Policy (CEPP) and KIITCIE.

2009

Best Happening Stall Award Winner in the Kritansh techno fest organized by KIIT University.

2008

ORAL AND POSTER PRESENTATIONS:

Rout, R., M. Suar, M. Tonolla, P. Luethy and V. Raina. “Evaluation of the larvicidal potential

of indigenous Bacillus thuringiensis isolates for control of vector-borne diseases” poster

presented at 53rd Annual AMI (Association of Microbiologists in India) conference in Odisha.

2012

Rout, R., V. Raina, M. Suar, and P. Luethy. Site directed isolation and characterization of

Bacillus thuringiensis strains active against mosquito larvae in the Indian state of Orissa. 6th

European Mosquito Control Association Workshop (EMCA), Hotel Helia, Budapest, Hungary. In

12-15 September 2011. (Oral Presentation)

Regalin R., M. Suar, V. Raina and P. Luethy. “Biocontrol of vector-borne diseases by

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indigenous isolates of Bacillus spp. from Orissa” poster presented at ISCB International

Symposium organized by Indo-Swiss Collaboration in Biotechnology in New Delhi, India. 2011.

Rout, R., M. Suar, V. Raina, R.K. Hazra, and P. Luethy. “Integrated vector management with

indigenous isolates of Bacillus thuringiensis israelensis (Bti): a key to the control of vector borne

diseases such as Malaria” poster presented at 50th Annual AMI (Association of Microbiologists

in India) Conference in New Delhi. 2009

Rout, R., M. Suar, V. Raina, R.K. Hazra, and P. Luethy. “Isolation of Indigenous Strains of

Bacillus thuringiensis israelensis (Bti) with a Potential for Integrated Malaria Vector

Management in India” poster presented at Society for Vector Ecology, Turkey. 2009

Rout, R., R.K. Hazra, M. Suar, V. Raina, and P. Luethy. “Isolation and preliminary

characterization of indigenous strains of Bacillus thuringiensis active against larvae of

mosquitoes” 49th Annual AMI (Association of Microbiologists in India) Conference in New

Delhi. 2008

Members:

• Life time member of Association of Microbiologists in India(AMI)

• Annual Member of American Society for Microbiology(ASM)

CONFERENCES AND WORKSHOPS ATTENDED:

2012- Workshop on “Scientific writing and publishing” organized by American Society for

Microbiology (ASM) during the 53rd AMI Conference.

2012- 99th National Science Congress organized and hosted by KIIT University and NISER.

2008- International Conference on “Emerging Trends in Biological Sciences”.

2008- “Kritansh”-the techno fest hosted by KIIT University.

2007- International Conference on “Lessons from Microbial World”.

Building Awareness on Solid Waste Management” organized by KIIT Centre for Innovation and

Entrepreneurships (KIITCIE), KIIT University. 2009

Research Communication for Societal Change” co-convened by the Centre for the Environment

and Public Policy (CEPP) and the KIIT Centre for Innovation and Entrepreneurships (KIITCIE),

KIIT University. 2008

Microbial Biotechnology and Bioinformatics” organized by Centre for Advanced Studies in

Botany, University of Madras, Chennai. 2005

REFERENCES:

1. Prof. em. Dr. Peter Luethy, Institute of Microbiology, Swiss federal Institute of Technology

(ETHZ), Wolfgang-Pauli-Strasse10; 8093 Zurich, Switzerland.

Email- acajkv@r.postjobfree.com, Mobile: +41-79-691-**-**, Landline: +41-44-923-**-**

2. Dr. Vishakha Raina, Associate Professor, School of Biotechnology (SBT), KIIT University,

Bhubaneswar-751024, Odisha, India.

Email: acajkv@r.postjobfree.com, Mobile: +91-993*******, Landline: +91-674-*******

3. Dr. Mauro Tonolla, Faculty of Science, Department of Plant Biology, Microbiology Unit,

Department of Botany and Plant Biology 30, quai Ernest-Ansermet CH - 1211 Geneva 4,

University of Geneva, Switzerland.

Email- acajkv@r.postjobfree.com, Mobile: +41-77-211-**-**, Landline: +41-91-814-**-**

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