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Curriculum Vitae

Dr. Balasubramanian Natesan, Ph. D.,

Formerly DST- SERB Scientist-D

School of Biological Sciences

Madurai Kamaraj University

Madurai-625 021

Tamil Nadu, India

Mobile No: +91-709*******

Email: adziok@r.postjobfree.com

Personal Profile

Nationality : Indian

Marital status : Married

Known Language : Tamil, English, Portuguese (Sufficient) Teaching Experience : 07 years

Research Experience : 16 years

Google Scholar Citations : 1250

: h-Index, 18

: i10-Index, 21

Scopus Citations : 681

: h-Index, 15

Researcher Id : M-3016-2014

Scopus Id : 572********

Orcid Id : 0000-0003-1819-0730

Post-Doctoral Research Experience

Designation Duration Institution

Scientist-D October 2015–

September 2021

School of Biological Sciences, Madurai

Kamaraj University, Tamil Nadu, India.

Post-Doctoral Fellow October 2013–

September 2015

CREM & Department of Life Sciences, New

University of Lisbon, Portugal.

Post-Doctoral Fellow January 2006 –

September 2013

CIRN & Department of Biology, University of

Azores, Portugal.

Post-Doctoral Fellow August 2005–

December 2005

Institute of Biological Chemistry,

Academica Sinica, Taiwan.

Post-Doctoral Fellow September 2004–

July 2005

Institute of Molecular Biology, National Chung

Hsing University, Taiwan.

Teaching Experience

Course Name Duration Place

M.Sc, Microbial

Gene Technology

July 2018 – June

2022

School of Biological Sciences, Madurai

Kamaraj University, Madurai, India.

Local Person Contact Address

C/O. Thiravium, R

Software Engineer

Suite 507,45 Balliol Street

Toronto, Ontario

Canada M4S1C3

Mobile No. +1-905-***-****

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M.Sc, Genomics October 2015–

February 2020

School of Biological Sciences, Madurai

Kamaraj University, Madurai. India.

M.Sc, Biomedical

Science

July 2020 –

December 2020

School of Biological Sciences, Madurai

Kamaraj University, Madurai, India.

B.Sc Biology &

Biotechnology

October 2006 –

September 2013

Department of Biology, University of Azores,

Portugal.

Academic Qualification

Degree Duration Institutiom

Ph.D (Biology) 1999–2003 Centre for Advanced Studies in Botany, University of Madras, India.

M. Phil (Biology) 1998–1999 Centre for Advanced Studies in Botany, University of Madras, India.

M. Sc 1996–1998 A.V.V.M. Sri Pushpam College (Bharathidasan University), India.

B. Sc 1993–1996 A.V.V.M. Sri Pushpam College (Bharathidasan University), India.

Research Interests

Domain : Microbiology, Clinical Microbiology,

Molecular Biology and Biotechnology

Current Area of Research : Streptococcal pathogenesis, virulence level against human and animals, Host pathogen interactions and

their molecular mechanism.

Fellowships

DST - SERB Ramanujan Fellow : Department of Science and Technology, Govt of India, New Delhi, 2015.

Post Doctoral Fellow : Foundation for Science and Technology (FCT), Portugal, 2013.

Post Doctoral Fellow : Directorate of Regional Science and Techology (DRCT), Azores, Portugal, 2006.

Post Doctoral Fellow : National Science Council, Taiwan. 2004.

Project Associate Fellow : UGC – INFHES, India, 2002.

Senior Research Fellow : Department of Biotechonlogy, India, 2001.

Senior Research Fellow : Department of Ocean Development, India, 1999. Awards and Honors

o Best Young Scientist : Received from PEARL A Foundation for Educational Award in Immunology Excellence on SMART Summit, 10 th

December 2016,

Madurai, Tamil Nadu, India, (Regn No:101/2015).

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o Travel Award : Received from FRCT, Azores, Portugal to attend International Conference on Genomics and Proteomics, Calicut, India, 2011

o Travel Award : Received from EMBO, Germany and FRCT, Azores, Portugal to attend C. elegans development and gene expression, EMBL-Heidelberg, Germany, 2010

o Travel Award : Received from DRCT, Azores, Portugal to attend Proteomics Europe, Barcelona, Spain,2009

o Travel Award : Received from DRCT, Azores, Portugal to attend Hostparasite interactions of Biology,

Rhode Island, USA, 2008

o Travel Award : Received from DRCT, Azores, Portugal to attend Proteomics Europe, Amsterdam, The Netherlands, 2007 o Gold Medal Prize : Best Student in Master of Science, 1998 o Proficiency Prize : Master of Science, 1997

o Proficiency Prize : Bachelors of Science, 1996

Principal Investigator - Research Projects Completed Funding agencey Duration Amount Project Title

DST-SERB, India October 2015–

September 2020

US$127,160 Studies on human disease causing

Streptococcus Pathogenesis and

their virulence factors

DRCT, Portugal November 2007 –

September 2009

US$23,000

Enhancement of nematode

activity and biophysical,

biochemical, molecular analysis of

serine protease and inhibitors

Contribution to GenBank (NCBI) Data Base: 31

Steinernema carpocapsae Gene Accession Numbers (9): Sc-ASP113 HM189211, Sc-ASP155 HQ412766, SPI-6 HM586104, SPI-2 HM586105, SPI-5 HM586103, Sc-ASP155 HQ709107, Sc-ASP113 JF317535, Sc-ASP110 JX392861, Sc-ASP110 JX499022. Streptococcus dysgalactiae subsp. dysgalactiae Gene Accession Numbers (8): VSD2 lytR KM485638, VSD6 lytR KM485639, VSD8 lytR KM485640, VSD9 lytR KM485641, VSD15 lytR KM485642, VSD17 lytR KM485643, VSD19 lytR KP308400, GCS-Mo lytR KM485644. Streptococcus pyogenes Gene Accession Numbers (6): GAP8 brpA KR780755, GAP58 brpA KR780756, GAP88 brpA KR780757, GAP826 brpA KR780758. NBMKU11 speB MF574207, NBMKU12 speB MF574208.

Streptococcus agalactiae and S. dysgalactiae Gene Accession Numbers (4): NBMKU28 lytR MK047646, NBMKU20 lytR MK047647, NBMKU19 lytR MK047648, NBMKU21 lytR MK047649.

Pseudomonas species Gene Accession Numbers (3):

VSMKU4036 Prna- MH491828, VSMKU3098 16S- MK226293, VSMKU309716S- MK226294. Streptomyces species Gene Accession Number (1)

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VSMKU1023 MN-396396.

Articles Published

Total Number of Publications : 37

High lighted Peer Reviewed Publications :

1. Balasubramanian N, Pounpandi P, Varatharaju G, Shanmugaiah V, Balakrisknan K and Thirunarayan MA. 2023. Distribution of virulence genes and biofilm characterization of human isolates of Streptococcus agalactiae: A pilot study. Colloids and Surfaces B: Biointerfaces. 223, Id. 113151, IF. 6.0 .

2. Priya VT, Balasubramanian N, Shanmugaiah V, Sathishkumar P, Kannan N.D, and Karunakaran C. 2021. Partially purified lead molecules from Dodonaea viscosa and their antimicrobial inhibitory efficacy against infectious human pathogens, Journal of Infection and Public Health, 14, 1822-1830. IF.7.53

3. Muthulakshmi L, Pavithra U, Sivaranjani V, Balasubramanian N, Sakthivel K, Catalin Iulian Prunce. 2021. Preparation and Characterization of a Novel Ag/Carrageenan–gelatin Hydrogel Hybrid Nanocomposite for biomedical applications. Journal of the Mechanical Behavior of Biomedical Materials. 115, doi: 10.1016/j.jmbbm.2020.104257. IF.4.04 4. Karmegham N, Vellasamy S, Natesan B, Sharma M.P, Al Farraj D.A, Elshikh M.S. 2020. Characterization of antifungal metabolite phenazine from rice rhizosphere fluorescent pseudomonads (FPs) and their effect on sheath blight of rice, Saudi Journal of Biological Sciences, 27

(12), 3313–3326. IF.4.05

5. Alves-Barroco C, Roma-Rodrigues C, Balasubramanian N, Guimaraes MA, Ferreira-Carvalho BT, Muthukumaran J, Nunes D, Fortunato E, Martins R, Santos-Silva T, Figueiredo AMS, Fernandes AR, Santos-Sanches I. 2019. Biofilm development and computational screening for new putative inhibitors of a homolog of the regulatory protein BrpA in Streptococcus dysgalactiae subsp.dysgalactiae. International Journal of Medical Microbiology, 309(3-4),169-181. doi:10.1016/j.ijmm.2019.02.001. IF.3.65

6. Kuleshevich E, Ferretti J, Santos Sanches I, Balasubramanian N, Spellerberg B, Efstratiou A, Kriz P, Grabovskaya K, Arjanova O, Savicheva A, Suvorov A. 2017. Clinical strains of Streptococcus agalactiae carry two different variants of pathogenicity island XII. Folia Microbiologica, 62(5), 393- 399. IF.2.62

7. Harikrishnan H, Shanmugaiah V, Balasubramanian N, Sharma MP, Kotchoni SO, 2014. Antagonistic potential of native strain Streptomyces aurantiogriseus VSMGT1014 against sheath blight of rice. World Journal of Microbiology and Biotechnology,30 (12), 3149-3161. IF.4.25 8. Balasubramanian N, and Simões N. 2014. Bacillus pumilus S124A carboxymethylcellulase: a thermo stable enzyme with a wide substrate spectrum utility. International Journal of Biological Macromolecules, 67, 132–139.IF.8.02

9. Toubarro D, Avila M.M, Hao Y.J, Balasubramanian N, Jing Y, Rafael M, Faria T.Q, Brito R.M and Simões N. 2013. A serpin released by an entomopathogen impairs clot formation in insect defense system. PLoS ONE 8(7), e69161.IF.3.75

10. Balasubramanian N, Nascimento G, Ferreira R, Martinez M and Simões N. 2012. Pepsin-like aspartyl protease (Sc-ASP155) cloning, molecular characterization and gene expression analysis in developmental stages of nematode Steinernema carpocapsae. Gene, 500 (2), 164–171. IF.3.91 Page 5 of 7

11. Balasubramanian N, HaoY.J, Toubarro D, Nascimento G and Simões N. 2009. Purification, biochemical and molecular analysis of a chymotrypsin protease with prophenoloxidase suppression activity from the entomopathogenic nematode Steinernema carpocapsae. International Journal for Parasitology 39(9), 975–984. IF.4.33

Books/Manual (4)

1. Shanmugaiah V, Balasubramanian N, and Varatharaju G. Theoritical manual on “Industrial Microbiology”

(DE III-MB T7), Directorate of Distance Education, Madurai Kamaraj University, 2016. 2. Shanmugaiah V, Balasubramanian N, and Varatharaju G. Practical manual on “Industrial Microbiology”

(DE III-MB T7), Directorate of Distance Education, Madurai Kamaraj University, 2016. 3. Shanmugaiah V, Balasubramanian N, and Jayaprakash J. Theoritical manual on “Soil, Agricultural & Environmental Microbiology" (UMBI05), Directorate of Distance Education, MK University, 2016. 4. Shanmugaiah V, Balasubramanian N, and Jayaprakash J. Practical manual on “Soil, Agricultural & Environmental Microbiology" (UMBI05), Directorate of Distance Education, MKj University, 2016. Book Chapter: (6)

1. Zarin Taj Z, Harikrishnan H, Shanmugaiah V, Suresh S, Balasubramanian N, and Muthuchelian K. Biosynthesis of silver nanoparticles from fluorescent Pseudomonads against phyto fungal pathogens. In Bionanosciences (Ed. K. Muthuchelian). Chapter 8, 145-152, 2015,Write and Print Publications, India. ISBN: 978**********.

2. Shanmugaiah V, Nithya K, Jayaprakasvel M, and Balasubramanian N. Biocontrol mechanisms of siderophores against bacterial plant pathogens. In Sustainable approaches to controlling plant pathogenic bacteria (Eds. V. Rajesh Kannan and Kubilay Kurtulus Bastas). Chapter 8, pp 167-189, 2016, CRC Press, USA.ISBN: 978-1-48-224053-5.

3. Gomathinayagam S, Balasubramanian N, Shanmugaiah V, Rekha M, Manoharan P.T and Laithakumari D. Molecular characterization of carbendazim resistance of plant pathogen (Bipolaris oryzae). In Fungicides-Beneficial and Harmful Aspects, (Eds.Nooruddin Thajuddin) chapter 11, pp197-218, 2011, InTech Publisher, Croatia. ISBN: 978-953-307-451-1. 4. Simões N, Toubarro D, Balasubramanian N, Nascimento G, Martinez M, Ying J, Hao Y.J and Montiel R. Bioinsecticidal ability of virulence factors released by Steinernema carpocapsae. In insect pathogens and entomopathogenic nematodes, IOBC/WPRS Bulletin Vol 66, 2011, pp291-294 (Eds. Ralf-Udo Ehlers, et al.,), 2011. Austria.ISBN: ***-**-****-241-8.

5. Simões N, Toubarro D, Balasubramanian N, Nascimento G, Martinez M, Ying J, Hao Y.J and Montiel R. Analysis of secreted/excreted products of Steinernema carpocapsae (Nemata). In Insect Pathogens and Insect Parasitic Nematodes,IOBC/WPRS Bulletin Vol 45, 2009, pp379-382 (Eds. Ralf-Udo Ehlers, JürgEnkerli, Itamar Glazer, Miguel-Lopez-Ferber &CezaryTkaczuk), 2009. France. ISBN: ***-**-****- 205-0.

6. Duarte A, Correia B, Balasubramanian N and Hao Y.J. Collection of soil samples in the islands of Flores and Corvo for isolation of entomopathogenic bacteria (Recolha De Amostras De Solo Nas Ilhas Das Flores E Corvo Para Isolamento De Bactérias Entomopatogénicas). In XIII Scientific Expedition of Department of Biology, Flores, Corvo, 2007 (Eds. Joao Antonio Candido Tavares and Duarte Soares Page 6 of 7

Furtado), Departamento de Biologia, Universidade dos Açores, Portugal. pp 35-40. ISBN: 978-***-****- 38-2.

Conference Proceedings: (4)

1. Balasubramanian N, and Simões N. 2013. “Role of aspartic proteases in Steinernema carpocapsae parasitism and development”. InJourney of Azores Science What Future?,(Eds. José Azevedo, Gilberta Rocha, Nelson Simões, Paulo Borges, Ricardo Santos and Pedro Raposeiro), Public library, Ponta Delgada, Azores, Portugal. pp 269-274.

2. Simões N, Toubarro D, Balasubramanian N, Teixeira M, Martinez M.A, Driss F, Ferreira R, Cabral C. 2013. “Research and Innovation in Bio-insecticides”.InJourney of Azores Science What Future?, (Eds. José Azevedo, Gilberta Rocha, Nelson Simões, Paulo Borges, Ricardo Santos and Pedro Raposeiro), Public library, Ponta Delgada, Azores, Portugal. pp 205-212. 3. Balasubramanian N, and Simões N. 2011. “Analysis of secreted proteases from entomopathogenic nematode Steinernema carpocapsae and role in host immune suppression”. In International Conference on Genomics and Proteomics, (Eds. Santhiagu, A.) School of Biotechnology, National Institute of Technology, Calicut, Kerala, India.pp 15-18.

4. Balasubramanian N. 2003. Improved biocontrol efficiency and rhizosphere competence in protoplast fusion progenies of Trichoderma spp. In Innovative Methods and Techniques for Integrated Pest and Disease Management (Eds. Mathivanan N., Prabavathy V.R., and Gomathinayagam S.), Centre for Advanced Studies in Botany, University of Madras, Chennai, India, pp.98-103. Papers and Posters Presented in Conferences/Symposium/Seminar National Level : 39

International Level : 43

Invited Lectures : 07

Workshop and Training Course attended :` 10

Membership in Scientific Bodies : 10

Brief Note on my Resrach Program

My doctoral research work was strain improvement by protoplasmic fusion in the Trichoderma fungal species for the enhanced lytic enzyme such as chitinase and cellulase production and biological control potential. I also analyzed proteins, DNA from these parent and fusant fungal strains. During my first postdoc period, I studied fusion protein with yeast AH109 cells, this fusion protein was identified by SDS-PAGE, western blot techniques and characterized for other activities. In the Second postdoc Steinernema carpocapsae nematode secreted serine, aspartic proteases and their role on host pathogenesis understood. Furthermore, gene expression using nematode developmental stages including infective juvenile stage was studied. Moreover, thermophilic Bacillus species was isolated from volcano soil samples and their cellulase, protease enzymes purified and characterized for the industrial applications. In the third posdoc, I moved to work on human and animal pathogenic bacteria Streptococcus species host specificity among Streptococcus species and their virulence mechanism on Page 7 of 7

pathogenesis. Different virulence factors, gene expression and protein were studied. The secreted cysteine protease studied to understand host pathogen Interaction. After joining as a Ramanujann Fellow (Scientist) in Madurai Kamaraj University, India, I have been continued to work on Streptococcus different species. Besides, different microbial and plant metabolites were checked antimicrobial activity against pathogens to understand resistance or susceptible phenotypes and genotypes. Based on my research experience, I would conduct research based on society issues like agriculture, healthcare and environmental aspects for the betterment of human life.

References

1. Dr. V. Shanmugaiah

Senior Assistant Professor

Dept. of Microbial Technolgy

School of Biological Sciences

Madurai Kamaraj University

Madurai-625 021, India.

Email: adziok@r.postjobfree.com

Dr. Dr. Thirumananseri Kumarevel

Senier Scientist

Structural Biology Laboratory

RIKEN Yokohama Institute

Yokohama 230-0045

Japan.

Email:

adziok@r.postjobfree.com

Dr. Nelson Simoes (Rtd)

Professor and Head

Department of Biology

University of Azores

Ponta Delgada-9501 801,

Portugal.

Email: adziok@r.postjobfree.com

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