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Research Experience Engineering

Gurgaon, HR, 121017, India
August 22, 2012

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D *** Celebrity Homes, Palam Vihar, Gurgaon,Haryana, India 121017

91-886******* •


Highly experienced science/biotechnology professional with nearly 20 years of hands-on research and teaching experience, including 15 years in the U.S. as a researcher for multiple educational institutions. Combines expertise in specific subjects, including genomics and proteomics tools and disulfide isomerases, with strong skills in key areas, from staff supervision and project management to presentations and training.

College-level Course Design & Instruction • Student Advisement • Staff Training & Management

Laboratory Equipment Procurement • Microbiology Research & Analysis • Project Management

Presentations & Seminars • Hindi, Punjabi, Urdu & English Fluency


MAHARISHI MARKHANDESHWAR UNIVERSITY, Mullana, Ambala, India • July 2008-Present

SINGHANIA UNIVERSITY, Bari, Rajastan, India • Jan 2008-July 2008. (Currently on Leave)


Maharishi Markhandeshwar: Develop, plan, and teach courses – Fundamentals of Microbiology and Applied Microbiology and Fermentation Technology Design of Vaccines and Clinical trials, for one of India’s largest private universities. Interact with 10 faculty members and contribute to recruitment of new staff and overall department management.

Singhania: Prepared and taught courses to more than 50 students in Biotech, Microbiology, Bioinformatics Principles, Genomics, and Proteomics for rural university. Provided hands-on counseling and assistance with job searches and interview strategies.

Major Highlights

Mahariski Markhandeshwar

• Designed and facilitated course of study for new Biotechnology program (Engineering discipline) in the Bachelor’s of Technology (BTECH) program.

• Played key role in recruitment of student (We have a preadmission couselling during which I guided them towards the benefits of the course they were taking-i.e. BTECH Biotechnology) – brought in over 100 students to BTECH program, and more than 25 to the MTECH program (Masters).

• Personally designed new teaching laboratory – gathered information from both online and professional sources, including consultations with eminent Indian scientists in (Industry and Academia).


• Successfully conducted three 6-month courses and supervised research projects for students.

• Invited to guide several Ph.D. thesis studies.

J. SARGAENT REYNOLDS COMMUNITY COLLEGE, Richmond, Virginia • 2006-2007

Community college offering 2-year degree programs, as well as certificate programs.

Adjunct Professor of Biology

Delivered BIOL 101 and BIOL 102 courses.

Major Highlights

• Earned accolades and appreciation from students by proactively engaging them in coursework and potential improvements to class administration.

• Guided Courses in Ecological and conducted field projects as well.


UNIVERSITY OF VIRGINIA, Charlottesville, Virginia, WI • 2004-2006

Public research university with 13K+ undergraduate students and 6K+ graduate students.

Research Associate

Supervised 2 students and 1 technician in genomic research focused on Identification and Characterization of BHV (broad host virulence) locus in Staphylococcus aureus using the Caenorhabditis elegans infection model.

Major Highlights

• Determined individual and cumulative contributions of members of the BHV locus to virulence and biofilm formation using the C. elegans infection model by generating single and double deletion knock outs of members of this locus.

• Worked with biothreat agent Franscicella tularensis.

CAREER NOTE: Previous history includes role as Junior Scientist for HMGENEInc.


UNIVERSITY OF MASSACHUSETTS, Worcester, Massachusetts • 1998-2000

Post-doctoral Associate

Conducted research on identification of contributions to Haemophilus influenzae pathogenesis by the dsbA ortholog por (Periplasmic oxidoreductase). Subsequent studies on the periplasmic fractions using single dimension gel sub-proteomics and mass spectrophotometric analysis revealed ribH as a downstream target of por.


Post-doctoral Associate

Conducted research to examine the nodulation process in the Microsymbiont Sinorhizobium melliloti and its host Medicago truncatula. Utilized advanced genomics and proteomics tools in set-up of the laboratory.


• 1992-2000

1996-2000 – Mentor: Prof. Dennis E. Ohman

Employed Proteomic and Genomic analysis to identify transcriptional and proteomic changes upon mucoid conversion in Pseudomonas aeruginosa strain PAO1. Analysis identified bona-fide targets of AlgT (Sigma-22, an E. Coli Sigma E ortholog) the dsbA gene and also a novel 15 kDa protein with unknown function. Additionally, identified targets-AruC (an arginine biosynthesis enzyme) in particular-that were repressed by AlgT. Subsequent characterization of DsbA showed that it was not required for alginate biosynthesis, suggesting that the AlgT regulon encompasses a large set of genes besides those involved in Alginate biosynthesis. Analysis has set the path for further genomic proteomic and systemic characterization of regulons in P. aeruginosa.

1992-1996 – Mentor: Dr. Lorraine Albritton

Conducted research focused on characterizing the receptor domain for the murine ecotropic virus receptor in mATRC1. Used homology scanning to identify residues that may be critical for receptor function. Subsequently, determined that alanine scanning mutagenesis and biochemical substitutions would help identify and delineate the biochemical role of these residues that were critical for function.



Doctor of Philosophy in Microbiology

University of Tennessee, Memphis, Tennessee

Degree in Zoology

Delhi University, Delhi, India

Graduate-level course in Bioinformatics – Virginia Polytechnic and State University


American Society of Microbiology Members (since 1998)


• Ajay Kumar and Sonal Malhotra. A Simple Method For Introducing -SH Group at 5' OH Terminus of Oligonucleotides. Nucleosides & Nucleotides 1992 11(5): 1003 - 1007. Copyright 1992, Marcel Dekker, Inc.

• Malhotra, S., A. G. Scott, T. Zavorotinskaya, and L. M. Albritton. Analysis of the Murine Ecotropic Leukemia Virus Receptor Reveals a Common Biochemical Determinant on Diverse Cell Surface that is Essential to Retrovirus Entry. Journal of Virology, Jan 1996; 70 (1): 321-6.

• Ohman, D.E., S. Malhotra, S. Jain, K. Mathee, and L. A. Silo-Suh. Alginate biosynthesis in Pseudomonas aeruginosa: a stress response. Clinical Microbiology 1999; 5:5S11-13.

• Malhotra, S., L. A. Silo-Suh and D. E. Ohman. A Proteome Analysis of the Effect of Mucoid Conversion on Global Protein Expression in Pseudomonas aeruginosa Strain PAO1 Shows Induction of Disulfide Bond Isomerase, DsbA. J Bacteriol. Dec 2000; 182(24): 6999-7006.

Malhotra, S, S. Suh and D. E. Ohman. A Proteome Analysis on Global Protein Expression in Pseudomonas aeruginosa Cystic Fibrosis Isolate FRD1, Shows Induction of a 15 kDa Protein that is Homologous to Escherichia coli Protein YgaU. (In preparation).

• Sonal Malhotra, Qiang Chen, Nicole J. Mcmaster and Bruno W. S. Sobral. A Thioredoxin Like Protein (tlpA) Ortholog in Sinorhizobium meliloti is Critical For the Nodulation Process (In preparation).

• Sonal Malhotra, Brian J. Akerley and Dennis E. Ohman: An examination of Disulfide Isomerase function in two Distinct Bacterial Systems Reveals Common Pathways of Action that may Control Bacterial Virulence. CETAS-2009 (Changing Environmental Trends and Sustainable Development) International Conference in Hissar India. February 9-11, 2009 Proceedings CETAS Page 344.

• Sonal Malhotra: Examination of a Genetic Basis for Leadership traits using Manic Depressive Illness as a Model. 16th EDIC National Conference on New Initiatives in Entrepreneurship and Industry-Institute Interface. September 16-17th 2009. National Institute of Technical Teachers Training and Research, Chandigarh, India.

• Sonal Malhotra and Ramesh Chand. Identification of Potential DNA sequences in Pseudomonas aeruginosa and other Food borne pathogens that could serve as potential as diagnostic markers. Accepted for Publication in Proceedings of National Conference on Hospitality Industry in India - Developments & Challenges: Nov. 6-7, 2009.

• Sonal Malhotra. New and Alternate ways to enhance efficient oil production from Algae and potentially Microorganisms. Accepted for Publication and Presentation at the NITTTR Conference on Cleaner Production Technologies. November 16th –November 17th 2009.

• Sonal Malhotra. Efficient Waste management of Liquid Oil from University and Hospital Cafeterias-A systematic Analysis. Accepted for Publication and Presentation at the NITTTR Conference on Cleaner Production Technologies. November 16th –November 17th 2009.

• Sardul Singh Sandhu, Anil K. Sharma, Vikas Beniwal, Gunjan Goel, Priya Batra, Anil Kumar, Sundeep Jaglan, A. K. Sharma and Sonal Malhotra: Review Article: Biocontrol of Insect Pests:

Factors involved, Mechanism and Regulation. Journal of Pathogens, HIndawi Publications, Vol 2012, Article ID 126819, 10 pages. Year of Publication 2012.


• Malhotra, S., A. Scott and L. M. Albritton. Biochemical Contribution of an Essential Tyrosine Residue in the MulV-E Receptor-SU Protein Interaction. Retrovirus Meeting at Cold Spring Harbor May 24-May 29, 1994.

• Malhotra, S., and D. E. Ohman. Use of a 2-Dimensional Gel Analysis Reveals Proteins in Pseudomonas aeruginosa whose Respective Genes are Regulated by the Alternate Sigma Factor AlgT. Annual American Society for Microbiology Meeting. Chicago IL May 1999.

• Malhotra S, Nicole J. McMaster, Eric Nordberg and Bruno W. S. Sobral. A Global Examination of the Nodulation Process in the Model Microsymbiont, Sinorhizobium meliloti and its host Medicago truncatula. Poster displayed at Congressional Meeting, Virginia Tech- Northern VA Campus, May 2002.

• Sonal Malhotra, Qiang Chen, Nicole J. Mcmaster and Bruno W. S. Sobral. A Thioredoxin Like Protein (tlpA) Ortholog in Sinorhizobium meliloti is Critical For the Nodulation Process. Abstract presented at Annual American Society for Microbiology Meeting, New Orleans, LA May 2004.

• Sonal Malhotra and Costi D. Sifri. Comparison of the Disulfide Isomerase dsbA genes Between Gram Positive and Gram Negative Bacteria Reveals Distinct Sequence Divergence in Conserved Domains. Poster presented at the Annual Research Day celebration. Dept. of Internal Medicine. University of Virginia at Charlottesville. May 3, 2005.

• Torres-Zapata, Irimar, UPR-MAYAGUEZ; Malhotra Sonal, Division of Infectious Diseases & International Health University of Virginia Health System; Sifri Costi D.,Division of Infectious Diseases & International Health University of Virginia Health System. Caenorhabditis Elegans: A Novel Host For Staphylococcus aureus Pathogenesis. 41st ACS Junior Technical Meeting & 26th Puerto Rico Interdisciplinary Scientific Meeting (PRISM), University of Puerto Rico at Cayey 2006.


• Research presentation to Prof. Sharon Long’s Research Lab, Dept. of Biological Sciences, Stanford University, Stanford CA. May 2002. Developed partnership with the Long lab group. We obtained several plasmid constructs to establish a molecular genetic lab at Virginia Tech to study the microsymbiont Sinorhizobium Meliloti.

• Lecture at Rocky Mountain laboratories, National Institute of Health, 903, South Street, Hamilton MT 59840. December 12, 2004. Title: The biological role of Disulfide Isomerases Revealed by Genomic Analysis. Guest of Dr. Olivia Steele Mortimer.

• Seminar presentation at Plum Island Disease Center, United States Department of Agriculture. Foreign Animal Disease Research Unit. USDA, ARS, PIADC. Title: Impact of bioinformatics and genomic biology on addressing keys biology problems- A case in point Disulfide Isomerases Examined by Genomic Analysis. Primary focus was examination of various methods to develop genomic and bioinformatics tools to study FMV virus and other biological systems at Plum Island. Guest of Dr. Louis Rodriguez.

• Discussion forum on Bird Flu and its socio-economic impact on The Asian Continent. June 22, 2006. Guest of Brigadier Khanna and Mr. M. M. Malhotra, members of Thinker’s Forum, 863, Sector 17, Faridabad, Haryana. India 121002.

• Seminar presentation at School of Biotechnology, Jawaharlal Nehru University, New Delhi, India. March 24th, 2008. Seminar topic: An Examination of the AlgT regulon in Pseudomonas aeruginosa, using genomic and proteomic strategies.

• Training seminar on Molecular Biology to Lecturers and Teachers of local Government Colleges. Academic Staff College, Jawaharlal Nehru University, New Delhi, India. August 6th 2010.

• Conference Talk: Green Biotechnology, Amity University Jaipur, Nov 11-13, 2011. Conference Talk and Abstract Presentation.

• Training Seminar on Molecualr Biology to Lecturers and teachers Academic Staff College, Jawaharlal Nehru University, New Delhi, India. August 10, 2011.

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