Apex, North Carolina
To secure entry level job in Biopharmaceutical Industry.
Research Assistant, Microbiology and Molecular Lab, Amrita institute of Medical Science, Kochi, India, Dec 2012 to Dec 2013
Prepare chemical solutions, reagents required for isolation of Plasmids, Genomic DNA and SDS -PAGE .
Preparation of media and cultures for laboratory analyses.
Maintenance of organisms in glycerol stock and quadrant streaking in Luria agar plates.
Preparation and storage of reagents.
Performed Polymerase chain reaction (PCR) test, Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) .
Stringently followed lab protocols, sanitation standards and testing procedures.
Perform other duties as assigned.
Quality Control Analyst, Wockhardt Biotech Ltd., Aurangabad (Maharashtra), India, Nov 2009 to Nov 2011
•Preparation of reagents required for testing of in-process samples, finished product, and stability samples.
•Perform tests for in-process samples, finished product and stability samples.
•Perform following test for Insulin Human and Insulin Glargine products :
Description and Solubility.
Assay by HPLC method.
Loss on drying.
Determination of Sulphated ash
Determination of Relative impurities by HPLC.
Determination of high molecular weight protein by HPLC.
Determination of Zinc by Atomic Absorption Spectroscopy.
Perform peptide mapping by HPLC.
Determination of residual Solvent by Gas Chromatography.
Determination of purity Insulin fraction by HPLC
Perform following test for Erythropoietin:
Peptide mapping by HPLC.
Determination of Dimer by HPLC.
Determination of productivity of Erythropoietin intermediates.
Perform stability study for insulin human and insulin glargine.
Ensure equipment are maintained in calibrated state.
•Perform calibration of following instruments:
Atomic Absorption Spectroscopy
Analytical and ultra-micro balance
•Preparation and verification of working standards.
•Plan schedules for testing along with manufacturing department.
•Peer Review of results.
•Participated in multiple regulatory and customer audits: ANVISA (Brazil), WHO, INVIMA, Kenya MOH, FDA-India.
Masters of Technology (M-Tech) in Biotechnology
Kalasalingam University, India, June 2007- May 2009
Bachelor of Technology (B-Tech) in lndustrial Biotechnology
Udaya School of Engineering, India, Anna University, June 2003 - May 2007
•Worked on” Biological Separation of Fats and Solids with Bio methanation Process for the treatment of Dairy Effluent using UASB Reactor” as M-Tech final year project dissertation work from 2nd January 2009 – May 4th 2009 National Institute for Interdisciplinary Science and Technology(CSIR) Trivandrum, India, under the Guidance of Dr. V.B. Manilal MSc;PhD.
•Worked on “Antitumor and Toxicity Studies of Elephantopus scaber L. Invitro and In vivo.” as B-Tech final year project dissertation work from15th December 2006 – 15th March 2007 - Regional Cancer Centre, Trivandrum, India under the Guidance of Dr. Ramani M.Sc., PhD.
•HPLC in Theory and Practice (Laboratory Certification) 16-hour course completed in May 2017 at Wake Tech, Raleigh, North Carolina.
•Elements of Current Good Manufacturing Practices (1.6ceus) 16-hour course completed in July 2017 at Wake Tech, Raleigh, North Carolina.
•Participated and presented a poster and won Best prize on “Beneficial Products from bio super foods (BSF)- Spirulina “in Perspectives and Future Trends in Biological Sciences” held from 7th to 8th January 05 at Selvam Arts and Science College, India.
•Participated and Presented a Poster and won Best prize on” Emergence of Fermented Foods” in “Recent Trends in Biotechnology of Fermented Foods” held from 13 to 15th July 05 at Agricultural University, India.
•HPLC -Agilent 1200, Dionex and Shimadzu with Chromeleon Ezchrome and Chemstation CDS software
•Gas Chromatography (Agilent)
•Atomic Absorption Spectroscopy (GBC)
•UV-VIS spectroscopy (CINTRA 10e)
•SDS (Tarson and Bio-Rad)
•PCR (G-storm and Eppendorf)
•Good Documentation Skills.
•Knowledge of GCP, GLP, GMP, ICH guidelines.
Microsoft Office (Access, Excel, Word, PowerPoint)