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Assay Development and Drug Discovery Preclinical Research Scientist

Location:
Auburn, Alabama, United States
Posted:
August 19, 2018

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Resume:

Career Objective

PhD Research Scientist with over 15 years of experience providing thorough and skillful support in drug discovery and assay development research.

Professional Experience

Vice-President Jan 2009 to Current

Vines Funeral Home - Lafayette, AL

Co-owner and Licensed Mortician/Funeral Director.

Achievements:

• Awarded Historic National Registry designation for the business.

• Major contributor in the designed and publishing of company’s webpage and brochures.

• Delivered on all interior design decisions for the company during a major renovation and/or restoration project of three buildings.

• Assembled and maintained the company’s OSHA required MSDS directory and gave a workshop for the staff on how to use it in case of an accident. REDDY US THERAPEUTICS/DR. REDDY'S LABORATORIES LTD - Norcross, GA Lead Scientist in the second generation drug target discovery program at the company from May 2000 to May 2009, including leading research projects to identify and characterize the MOA of novel small compounds and provided the proof-of-concept data for new drug targets which many times called for me to design and develop in vitro assays.

Principal Scientist May 2004 to May2009

Sr. Scientist Jun 2002 to Jun 2004

Scientist May to 2000-Jun 2002

My primary duties included working on three main projects: Glycogen Synthase Kinase 3-beta (GSK), Lipoic Acid Synthase (LASY), Suppressor of Cytokine Signaling (SOCS), and one on-going project, new second generation targets such as analgesia targets. Developed screens for target validation and organized collaborations with vendors and experts. Remained updated on literature and prepared manuscripts. Presented results to colleagues, completed safety and radioactivity training and managed resources for projects. Achievements:

• GSK: Published research data for the target, Glycogen Synthase Kinase-3beta, in the Journal of Biological Chemistry and obtained a patent on the methods, using the data. The work included cell line development for adenovirus infected cells expressing GSK-3 beta.

• GSK: Won Best Scientific Publication in 2006 for my original Journal of Biological Chemistry paper in our company-wide competition.

• GSK: Oral presentation at American Heart Association scientific meeting and several poster presentations at national meetings including the American Diabetes Association and American Heart Association.

• LASY: Developed a novel sandwich ELISA that unequivocally validated the company's lead diabetes compounds.

• LASY: Established a high throughput tissue processing station in the lab and right sourced the essential equipment and supplies, wrote SOPs and trained staff in the use of the equipment. ANGELA

VINES

225 B. Street S.W., Lafayette, Alabama 36862

334-***-****

ac6p86@r.postjobfree.com

https:/www.linkedin.com/in/scientistvinesangela

VINES 2

• SOCS: Independently investigated and championed a new drug target which was moved forward as an approved departmental project using my proof-of-concept data (i.e., Suppressor of Cytokine Signaling-3).

• Successfully increased the company's brand visibility at medical meetings by delivering presentations.

• Novel Targets: Validated 80+ novel drug targets through a battery of methods and protocols that were carried out, such as western blotting, viral gene expression, tissue culture, Protein/DNA/RNA isolation and purification, PCR, ELISA, anti-sense treatments, overexpression, time-course treatments, and animal experiments, while using the appropriate equipment and conducting the statistical analysis of the data.

• Novel Targets: Validated 75% of the new drug targets and averted a $1M investment fiasco when my modified approach to our validation process was implemented...

• Novel Targets: Co-wrote the Target Product Profile or TPP for several analgesia targets so as to provide focus for the development team.

Visiting Sr. Research Scientist May 1998 to Dec 1999 Merck and Co. - West Point, PA

My duties included studying the immune response in guinea pigs which produces a very robust T cell response, like humans, against intracellular pathogens; and, developing guinea pig reagents which are lacking. The goal was to do molecular immunological studies in the guinea pig animal model for pre-clinical vaccine development.

Achievements:

• Performed early development work on vaccines to Haemophilus influenza, Moraxella catarrhalis, and Staphylococcus aureus to elicit an authentic antigenic response.

• Oversaw 85+ recombinant proteins that were processed in the bacterial vaccine study, including designing gene-specific PCR primers; PCR amplifying the genes using known hamster genes sequences as templates; performing sequence analysis on all the PCR amplicons; and, in vitro translated the encoded proteins.

• Initiated purification and refolding by using dialysis of many of the proteins to ensure proper immune recognition.

• Determined the nucleotide sequence of guinea pig gamma interferon, previously not known, and seven additional unexpected guinea pig genes.

• Supervised and mentored 1 report in the in vitro translation of the 85+ recombinant proteins generated from the bacterial vaccine study, which required cell line development for each recombinant protein.

• Evaluated immune responses in different lymphoid cell populations using flow cytometry, fluorescence imaging, and ELISPOT for various in-house experiments to detect antibody-producing B cells. Model Institute of Excellence Scholar Teacher Sept 1996 to May 1998 Spelman College-Atlanta, GA

Temporary faculty position to gain exposure to teaching and research responsibilities at an undergraduate institution.

Major Achievements:

• Instructor for the General Biology lecture and laboratory courses. Classes ranged in size from 20- 75 students.

• Investigated the verotoxin receptor’s role in the virulence of Escherichia coli using flow cytometry.

• Conceived and supervised research for two undergraduates and supervised another in science literature review.

• Mentored my research student in the highly competitive science day competition, and she won second place in the oral presentation.

• Presentations (2 total) at American Society of Microbiology Research Fellow Sept 1993 to Sept 1996

St Jude Children's Research Hospital- Memphis, TN

Since the influenza A virus HA has a major role in the virulence, its receptor specificity (measured by hemadsorption) and host range restriction changes (evaluated in vivo) were studied. Achievements:

VINES 3

• Used state-of-the-art virology/molecular biology methods and techniques, such as reverse genetics, cloning, PCR, and mutagenesis in studies to further our understanding of how pandemic influenza strains are derived and cause pathobiology.

• Routinely communicated research findings in departmental workshops (usually more than 15 attendees).

• Used bioinformatics data analysis to study nucleotide sequence changes in influenza viruses and evaluated 2 critical mutation sites previously shown in human virus HA to be important for virus replication in duck intestine (i.e., considered all possible amino acid changes at position 226 and 228 of the HA).

• FACS analysis and the novel hemeadsoprtion assay that I developed were used to screen for functional HA cell surface expression since this glycoprotein is what binds the virus to host cells in vivo.

• For receptor specificity studies, I used the HA constructs (23 functional constructs out of 24 were identified by FACS analysis) to characterize. For host range restriction, I used reverse genetics to make engineered viruses containing the different mutated HAs which were then tested in ducks.

• Showed that the Ser-to-Gly mutation at position 228, in addition to the Leu-to-Gln mutation at position 226 of the HA of the H3 subtype, is critical for human virus HA to support virus replication in duck intestine.

Knowledge, Skills and Interests

• Knowledgeable and experienced in Cell and Molecular Biology, Microbiology, Virology and Immunology.

• Laboratory management, mentoring and supervisory experience.

• Tangible/Intangible skills, including: Budgeting, Vendor Relations, Cost Reduction, Good communication skills, such as: organizational, planning, decision making, interpersonal, verbal, written, record keeping, data presentation, supervisory and computer skills (Microsoft Office software).

• Gene therapy, vaccine development and inflammation mechanisms interest me. Relevant Technical Proficiencies:

• Tissue culture (primary and immortalized), DNA sequencing and sequence analysis, SDS/PAGE, Western blotting, ECL, in vitro immunoassay development, siRNA, and virus cultivation.

• ELISA, cell-based assays (e.g. cell proliferation assays, signal transduction assays, and gene reporter assays), methods development, immunohistochemistry, and flow cytometry.

• Cloning, DNA/RNA isolation and purification, protein expression and purification, PCR, Real-time RT-PCR, quantitative RT-PCR, mutagenesis, cell transfections, microscopy, luciferase assays, primer design, design/construction of vectors, cDNA library construction, microarrays, assay validation and optimization, and various animal models such as diabetes mouse models. Education

PhD, Biology 1993

Clark Atlanta University, Atlanta, GA, United States GPA: 3.753 of a maximum 4

Major: Biology (Specializing in Cell and Molecular Biology) Honors: Cum Laude

Relevant Coursework and Training:

Advances in Biochemistry I/II and Molecular Genetics, Developmental Biology, Medical Microbiology (Audited at Georgia State University). Advance training in nucleic acid and protein sequence analysis at Pittsburgh Supercomputing Center, Carnegie Mellon University.

MS, Biology 1987

Clark Atlanta University, Atlanta, GA, United States VINES 4

Publications

Padmalayam, I., Hamm, R. Reddy, S., Bhuniya, D., Vines, A., Seenu, S., Chakrabarti, R., Saxena, U., Pillarisetti, S. (2012). Identification of a Pharmacological Inducer of Lipoic Acid Synthase that Impacts Mitochondrial Function: Metabolic benefits and Body Weight Loss without Changes in Caloric Intake. J Diabetes Metab 2012, S:11.

Vines A, Cahoon S, Goldberg I, Saxena U, Pillarisetti S. “Novel Anti-inflammatory Role for Glycogen Synthase Kinase-3 beta in the Inhibition of Tumor Necrosis Factor-alpha-and Interleukin-1beta- induced Inflammatory Gene Expression.” Journal of Biological Chemistry. Vol. 281: 169**-*****, 2006.

“Methods and Compositions for the Treatment of Inflammatory Disease.” US Patent 6,900,041 B2- Granted May 31, 2005.

Ito T, Kawaoka Y, Vines A, Ishikawa H, Asai T, Kida H. “Continued circulation of reassortant H1N2 influenza viruses in pigs in Japan.” Arch. Virology 143:1773-1782, 1998. Vines A, Well K, Matrosovich M, Castrucci MR, Ito T, Kawaoka Y. “The role of influenza A virus hemagglutinin residues 226 and 228 in receptor specificity and host range restriction.” J.Virol. 72:7626-7631, 1998.

Vines A, Swaminathan B. “Identification and characterization of nucleotide sequence differences in three virulence-associated genes of L. monocytogenes strains representing clinically important serotypes.” Curr. Microbiol. 36:309-318, 1998.

Vines A, Swaminathan B. “Nucleotide sequence analysis of two virulence-associated genes in Listeria monocytogenes serotype 1/2b and comparison with the same genes in other serotypes.” Letters in Appl. Bact. 24:166-168, 1997.

Ito T, Susuki Y, Mitnaul L, Vines A, Kida H, KawaokaY. “Receptor specificity of Influenza A viruses correlates with the agglutination of erythrocytes from different animal species.” Virology 227:493- 499, 1997.

GPA: 3.6 of a maximum 4

Major: Biology

Honors: Cum Laude

Relevant Coursework and Training:

Medical Microbiology (taken at Morehouse School of Medicine), Biochemistry, Cell Biology, and Advances in Biochemistry, Molecular/Cellular and Immunology. Advance Training in Cell and Molecular Biology at Catholic University and Methods in Monoclonal Antibody Development.

AS, Mortuary Science 1982

Gupton Jones College of Mortuary Science, Atlanta, GA United States GPA: 4.0 of a maximum 4

Major: Mortuary Science

Honors: Highest Honors

Relevant Coursework and Training: Microbiology, Anatomy and Accounting BS, Biology 1980

Tuskegee University, Tuskegee, AL, United States

GPA: 3.85 of a maximum 4

Major: Biology

Honors: Magna Cum Laude

Relevant Coursework and Training: Microbiology, Chemistry, Molecular Biology Transmission electron microcopy experience (Sr. Research Project). VINES 5

Vines A, Swaminathan B. “Nucleotide sequence analysis of two virulence-associated genes in Listeria monocytogenes serotype 1/2b and comparison with the same genes in other serotypes.” Letters in Appl. Bact. 24:166-168, 1997.

Ito T, Susuki Y, Mitnaul L, Vines A, Kida H, KawaokaY. “Receptor specificity of Influenza A viruses correlates with the agglutination of erythrocytes from different animal species.” Virology 227:493- 499, 1997.

Vines A, Reeves MW, Hunter S, Swaminathan B. “Restriction fragment length polymorphism in four virulence associated genes of Listeria monocytogenes.” Res. Microbiol 143:281-294, 1992. Abstracts:

Ricci A, Cahoon S, Vines A, Saxena U, Pillarisetti S. Glycogen synthase kinase 3 beta inhibits smooth muscle cell proliferation by modulating AP-1 activity. Presented at the American Heart Association, Chicago, Illinois, June 17-20, 2002.

Vines A, Cahoon S, Saxena U, Pillarisetti S. A novel anti-inflammatory role for GSK3 beta: Inhibition of TNF-alpha and IL-1 beta induced inflammatory gene expression downstream of NF-kappa-B transactivation. Presented at the American Heart Association, Chicago, Illinois, June 17-20, 2002. Pillarisetti S, Cahoon S, Vines A, Saxena U. Hyperinsulinemia markedly exacerbates glycated albumin and TNF-a induced expression of endothelial inflammatory molecules MCP-1 and VCAM1: A mechanism for accelerated atherosclerosis in insulin resistance Type II diabetes. Presented at the American Diabetes Association, Philadelphia, Pennsylvania, June 22-26, 2001. Cahoon S, Vines A, Saxena U, Pillarisetti S. Synergistic Induction of endothelial IL-6 by diabetic stimuli: An initiating event in diabetic nephropathy. Presented at the American Diabetes Association, Philadelphia, Pennsylvania, June 22-26, 2001.

Reagans K, Maloney M, Vines A. Evaluation of B cell adhesion molecules for homotypic adhesion in Daudi (Gb3+) and Daudi-derived VT500 (Gb3-) cells. Presented at the MIE Science, Engineering and Mathematics Day at Spelman College, Atlanta, GA, March 1998. Vines A, Maloney M. Role for verotoxin receptor (Gb3) in B cell adhesion. Presented at the American Society of Microbiology Meeting, Miami, May 1997. Vines A, Swaminathan B, Schuchat A. Heterogeneity in the structural genes encoding listeriolysin O in three serotypes of Listeria monocytogenes that cause human disease. Presented at the American Society of Microbiology Meeting, Dallas, Texas, May 1991. Vines A, Reeves MW, Hunter S, Swaminathan B. Restriction fragment length polymorphism in genes associated with virulence in Listeria monocytogenes. Presented at the International Conference Listeria and Food Safety, Laval, France, June 13-14, 1991. Vines A. Characterization of the phenotype of alkaline phosphates positive marginal zone lymphocytes in mouse spleen. Presented at the 4th Annual Nabrit Biomedical Symposium, Clark Atlanta University, Atlanta, Georgia, 1987.

Vines A. Simultaneous demonstration of alkaline phosphatase and antigen markers in frozen sections. Presented at the 6th Annual Immunology Conference, Toronto, Canada, 1986. Vines A. The production of monoclonal antibody against P815 tumor cells. Presented at BDSA Spring Symposium, Clark Atlanta University, Atlanta, Georgia, 1985. Vines A. The effect of microtubule disruptive drugs on macrophages: A transmission electron microscope study. Presented at the 8th Annual Minority Biomedical Research Support Meeting, Atlanta, Georgia, 1980.

VINES 6

Additional Information

THESES/DISSERTATION:

“Molecular characterization of three virulence-associated genes from serotypes of Listeria monocytogenes important in human disease.” (PhD) Comment: In the final stretch of my PhD work I was “scooped” by a group out of Denmark so I had to redirect and redefine my research which resulted in 2 additional years of research that was done at the Centers for Disease Control in Atlanta. Advisor. Dr. Bala Swaminathan

“An immunohistochemical characterization of the alkaline phosphatase positive cells in frozen sections of lymphoid tissues.” (MS) Comment: In my Advisor’s absence (health issues), I seamlessly took over most day to day responsibilities for running the lab. All my research meetings with my Advisor were carried out by teleconferencing. This was a major challenge that we achieved, considering the visual nature of my novel and obligatory double staining assay. Advisor. Dr. Judith Lumb

OTHER:

Research Associate-Centers for Disease Control and Prevention-1987 to1988; monoclonal and hybridoma cell line development using NS0, SP2 and etc. Bootcamp Survivor-The Auburn University Bioinformatics Bootcamp held May 11-15, 2015 at Auburn University.

WORKSHOPS & PRESENTATIONS:

Presenter, Liquidia Technologies, Morrisville, NC

Presenter, Tuskegee University, Tuskegee, AL

Attendee, Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference, Denver, CO Attendee and Presenter, ADA: Philadelphia, PA and San Francisco, CA Speaker, American Heart Association, Chicago, IL

Merck & Co. professional development classes: Immunology; Molecular Biology; Conflict Management Training; and, Real Time PCR Workshop Pandemic influenza: Confronting a re-emergent threat, Bethesda, MA Nucleic acid and protein sequence analysis, Pittsburgh Supercomputing Center, Pittsburgh, PA Immunochemistry Workshop, Catholic University, Washington DC The study of Chlamydia (Workshop), Centers for Disease Control, Atlanta, GA Presenter, Georgia State University, Atlanta GA

Presenter, Neose Inc., Philadelphia, PA

Presenter, Merck & Co., West Point, PA

Presenter, University of Georgia, Griffin and Athens, GA Presenter, Spelman College, Atlanta, GA

Presenter, Case Western Reserve University, Cleveland, OH Presenter, St. Jude Children's Research Hospital

HONORS & AWARDS:

Madison Who’s Who

Best Scientific Publication Award, Dr. Reddy’s Laboratories Summer Internships at Merck & Co. (2)

Nominated to Who’s Who in the South and Southwest

Recipient of the Patricia Harris Fellowship, 6 years Outstanding Employee Contribution Award, Centers for Disease Control and Prevention Nominated to the National Dean’s List

Advance Training Award in Cell and Molecular Biology, Catholic University Recipient of First Place Research Award, Clark Atlanta University Recipient of First Place Research Award, Tuskegee University –Sigma Xi Graduated Magna Cum Laude, Tuskegee University



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