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RESEARCH PROFESSIONAL

Location:
Summit, New Jersey, United States
Salary:
60000
Posted:
January 17, 2018

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Supriya Baviskar

*** *********** ***, *** ***, Summit, New Jersey 07901. Contact: +1-908-***-****

E-Mail: ac32sz@r.postjobfree.com

Summary

Over 6+ years of experience in Biopharmaceuticals industry.

Adept in Cell biology, Molecular biology and Protein biochemistry techniques.

Experience in Stem cell Department and Plasma Protein purification cGMP facility.

Completed Advance Diploma program in Biotherapeutics from Reliance Institute of Life Sciences.

Passed National Eligibility Test-LS and State Eligibility Test exam in Life science subject.

Self-motivated with a keen understanding of the latest research in biotechnology, and an inherent passion for learning.

Good communication and presentation skills.

Work Experience

Sep 2015 - Sep 2016

Worked as a Research Officer in In-vitro Biological Research Dept. under Dr. Indrani Sarkar (Principal Scientist), Glenmark Pharmaceuticals, Navi Mumbai

Responsibilities:

Performed RNA Isolation from cultured cells and animal tissues.

Synthesized cDNA from extracted RNA.

Performed Genomic PCR, RT-PCR to check level of biomarkers.

Carried out gene and recombinant DNA cloning, Sub cloning to expression vectors.

Generated Stable cell lines.

Prepared Competent bacterial cells, transformed and isolated plasmid DNA.

Mar 2013 - Aug 2015

Worked as a Senior Research Fellow in Cell Biology Dept, under Dr. Jomon Joseph; Scientist F; in National Centre for Cell Science, Pune.

Project: Exploring the functional connection between Par polarity proteins and Nup358 in Cell polarity

Responsibilities:

Checked the miRNA pathway member’s interaction with Nup358 protein.

Studied the regulation of miRNA during stress condition to the cells.

Assessed functional connection between some important proteins like dishevelled protein to cellular stress response through cell signalling.

Carried RNAi (with siRNA, shRNA) transfection assays to knockdown the protein expression.

Performed Western blotting to check the protein level after overexpression or knockdown of gene.

Performed Immunoprecipitation assays to check the interaction of overexpressed proteins with miRNA pathway members.

Checked the interaction of overexpressed protein with the polyribosomal complex by Polysome profile analysis.

Assessed gene expression by Dual Luciferase reporter assay.

Aug 2010 - Jan 2012

Worked as Executive - Operations in Reliance Life Sciences Pvt. Ltd. Navi Mumbai in Plasma Protein Purification cGMP facility.

Responsibilities:

Manufactured Plasma proteins (Albumin and IgG) in highly automated commercial scale plasma fractionation manufacturing plant.

Carried out ultra-filtration, centrifugation and chromatography during process.

Optimized, improved & scaled up purification process for cGMP manufacturing.

Troubleshooted & assessed: Process optimization, purification of plasma protein and CIP issues

DCS (Distributed Control System- Siemen’s) operation.

Validated the cleaning of process tanks

Documented different batches (Standard Operating Protocol & Batch Manufacturing Records)

Managed & trained team of production executives to fractionate plasma in an aspect environment

Apr 2009 - Jul 2010

Worked as Research Fellow in Reliance Institute of Life Sciences Pvt. Ltd. Navi Mumbai in Plasma Protein Purification cGMP facility.

Manufactured Plasma proteins (Albumin and IgG) in highly automated commercial scale plasma fractionation manufacturing plant.

Cultured the Umbilical cord derived mesenchymal stem cells.

Analysed the biomarkers using Flow cytometric analysis.

Performed cell differentiation experiment on derived Mesenchymal stem cells. Analysed the biomarkers using Flow cytometric analysis.

Aug 2008 - Jul 2009

Advance Diploma program in Biotherapeutics in Haematopoietic Stem cell, Regenerative Medicine group from Reliance Institute of Life Science, Navi Mumbai

Project: Derivation and Angiogenic differentiation of Umbilical cord Derived Mesenchymal stem cells

Isolated mesenchymal stem cells from umbilical cord by classical and enzymatic digestion method.

Established primary cell cultures.

Cultured the Umbilical cord derived mesenchymal stem cells.

Analysed the biomarkers using Flow cytometric analysis.

Performed cell differentiation experiment on derived Mesenchymal stem cells.

Regularly carried out cryopreservation, thawing of cells.

Carried out detection of Mycoplasma in UCMSC culture by PCR and gel electrophoresis method.

Immunohistochemical and Immunocytochemical staining of cells and tissues

Publications

Published “Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC” in ‘EMBO Reports’, Dec 30, 2016;

Manas Ranjan Sahoo,, Swati Gaikwad, Deepak Khuperkar, Maitreyi Ashok, Mary Helen, Santosh Kumar Yadav, Aditi Singh, Indrasen Magre, Prachi Deshmukh, Supriya Dhanvijay, Pabitra Kumar Sahoo, Yogendra Ramtirtha, Mallur Srivatsan Madhusudhan, Pananghat Gayathri, Vasudevan Seshadri & Jomon Joseph*

DOI 0.15252/embr.201642386 (2016 Impact factor – 7.739).

Achievements

.

Advance Diploma in Biotherapeutics from Reliance Institute of Life Sciences, a subsidiary of Reliance Life Sciences Pvt. Ltd. Navi Mumbai in 2008-2009

Cleared CSIR-UGC NET LS 2014 in Life Sciences subject.

Cleared GATE 2013.

Cleared State Eligibility Test examination 2013 in Life Sciences subject.

Academic Qualifications

Jul 2006 - Apr 2008 University of Mumbai/ Birla College of Arts, Commerce and Science

Course Name: Masters of Science in Biotechnology

Jul 2003 - Mar 2006 University of Mumbai/ Birla College of Arts, Commerce and Science

Course Name: Bachelors of Science in Biotechnology

Feb 2003 Maharashtra State Board/ V.G.Vaze College

Course Name: Higher Secondary School Certificate

Mar 2001 Maharashtra State Board/ Model English High School

Course Name: Secondary School Certificate

Training and seminar-

International symposium on “Recent advances in Biotherapeutics” at Reliance Life Sciences Pvt. Ltd. From: 13 Feb’09 to 14 Feb’09

Applied Genetics & Genetic Counseling” In Centre for Research in Mental Retardation, Malad. PG Research Lab-University of Mumbai. From: 27 to 30 Sep’06.

“Training on Immunological Techniques” Immunology Department, Jaslok Hospital, Mumbai. From: 26 to 29 Oct’06.

Personal Information

Date of Birth : July 01, 1986

Gender : Female

Marital status : Married



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