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Assistant Professional Experience

Philadelphia, Pennsylvania, United States
August 31, 2017

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Zhongling Li

**** ****** ***** ******, **** of Prussia, PA 19406



MS, Pharmaceutical Science Aug 2015-May 2017

TEMPLE UNIVERSITY, The School of Pharmacy (GPA: 3.95)

BS, Life Science and Technology Sep 2011-Jun 2015

CHINA PHARMACEUTICAL UNIVERSITY (GPA: 3.89; Ranking Top 1 in department)


Technical Skills:

Downstream processing development: Affinity chromatography Akta avant (GE health) method setting and system controlling, Protein A column packing, Filtration centrifugation, Clarified un-processing Broth preparation (MabSelect Sure), mAb product titration and concentrating, Caliper GX chip protein quality and quantity analysis, Nanodrop, SoloVPE protein concentration test.

Analytical: Size-exclusion chromatography(SEC), Caliper GX chip protein quality and quantity analysis, Nanodrop, SoloVPE protein concentration test, Size distribution/zeta-potential analysis, HPLC, UV-Vis, capillary chromatographic analysis, dissolution test, encapsulation efficiency detection, vacuum filtration, SDS-PAGE and ELISA.

Biological and cell culture: Cell culture media and buffer preparation, hybridoma cell subculture, cell density and viability analysis, and sterile operation.

Certification: SAS Base Programming Certification (License Number: BP067732V9) Feb 2017

Statistics Skills: SPSS, R


Scientific Student Worker Jun 2017-current

GSK Downstream Process Development Biopharm R&D

Optimized the protein A chromatography step in mAb processing platform by exploring the relationship between input parameters (DBC, elution pH, elution concentration) and responses (eluates’ pH, conductivity, yield, product quality).

Packed a 0.66* 25.8cm protein A column, wrote controlled method in Akta avant of project: Evaluation of alternate Protein A elution buffers for better control of viral inactivation pool pH.

Purified the clarified un-processing broth by Protein A. Detected eluates’ pH and Conductivity by pH/Cond. probe, detected aggregation %, HCP %, LMW % by Size-exclusion chromatography (SEC), detected mAb titer by SoloVPE and Protein A chromatography and concentrated eluates by filtration centrifugation.

Detected the molecular weight, quality and quantity of protein by Caliper GX. Analyzed the data by Labchip GX Reviewer.

Research Assistant Jan 2017-Jun 2017

Temple University Lab of Advanced Drug Delivery

Reviewed and evaluated the status and features of current nano-structure lipid carriers.

Utilized solvent evaporation technology to manufacture the PEG-DSPE and Tripalmitin/DHA based polymer-lipid hybrid nanoparticles(TPL-NPs); encapsulated the nanoparticles with small molecular chemo-drug to increase the half-life of chemo-drug from 15min to 10 hours and reduce the toxicity by enhancing the specific bio-distribution.

Encapsulated insulin into viscosity-enhanced nanocarrier (VEN) to increase the stability of its oral delivery.

Characterized the TPL-NPs by multiple analytical methods including dissolution test, encapsulation efficiency by reverse phase HPLC; size distribution and zeta-potential analysis by dynamic light scattering using Nano ZetaSizer, and morphology analysis by TEM.

Research Assistant Mar 2015-Jul 2015

Chengdu Di’ao Pharmaceutical Group Co Ltd

Performed cell clone selection and cell culture media optimization as a part of cell line development in T-75 flasks; analyzed the cell culture parameters by Biochemical Analyzer (NOVA); monitored the cell density and viability by auto-counter and hemacytometer.

Purified monoclonal antibody (Omalizumab) from hybridoma cells culture in 7L shaking-table; analyzed the purity of antibody by SDS-PAGE and determined the protein quantity by ELISA.

Research Assistant Apr 2014-Aug 2014

Beijing Tri-Prime Genetic Engineering Co Ltd

Developed a colorimetric method to determine the concentration of antimicrobial preservative (m-cresol) in α1b interferon injection; compared the results with HPLC. Greatly lowered detection cost for m-cresol by 80%.

Validated the method and transferred it to QC department.


National Scholarship 2012 & 2013

Top 0.2% of the nation, the highest academic scholarship by China Ministry of Education

1ST Award of National College Student Innovation Experiment Project 2014

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