Manish Thakran

**** ******* **** ** *** # ***, Bryan TX 77801

y9c4l0@r.postjobfree.com, Ph: +1-469-***-****

Education:

Masters in Biotechnology (PSM) Aug’11-Present

Texas A& M University College Station 3.4 GPA Expected Graduation Dec’12

B.Tech (Biotechnology) Aug’07 - Jun’11

University Institute of Engineering and Tech,

Kurukshetra University, Kurukshetra, India 3.76 GPA

12th Central Board of Secondary Education Apr’05-Apr’07

Bhatnagar International School New Delhi with 83%

Experience: Graduate student researcher

• Aerosol Technology Lab-Mechanical Engg Dept. Texas A & M University

Quantitation of Aircraft Fuel degrading microbes by culturing, microscopy and RT-PCR and Coluter counter.

Tested a Wetted wall cyclone for bioaerosol sampling using surrogate bacteria.

• Assisting Dr. Colin Young in development of animal models (mice) for Epilpesy and Multiple Sclerosis.

Immuohistochemistry of mice brain samples

RT-PCR for murine cytokines

Technical Skills:

• Microbiological culture media and animal cell culture (Lymphocytes) and media preparation

• Isolation and culture of microbes from different sources (water, soil and food), spreading and pouring

• Electrophoresis (Agarose Gel, PAGE and SDS-PAGE)

• Rapid Immunoassays, Immunoprecipitation, Agglutination Reactions, ELISA, Southern and western blotting

• DNA isolation, PCR, RT-PCR, RFLP and RAPD

• Flow cytometry on BD® FACS Calibur and Attune Acoustic Focusing Flow cytometer

• Computer Proficiency: MS Office, C & C++

Projects Undertaken & Summer Trainings:

Opexa Therapeutics Ltd, The Woodlands, Texas (Intern) May-August’12

• Developed an Immunophenotyping protocol to enumerate absolute T cell subtypes for a T cell therapy for treatment of Multiple Sclerosis

• Learned to operate Attune Acoustic Focusing Flow Cytometer which is a recently introduced dual laser 6 color 8 parameter Flow cytomerty machine.

• Also performed customized color compensation for different phenotyping panels.

• Learned to work on FlowJo: software package for analyzing flow cytometry data.

• Maintained lab inventory and stocked reagents.

Dr RML Hospital New Delhi in microbiology lab (Intern) Jul-Aug’09

Prepared culture medium, Performed, analyzed and reported blood, urine and stool culture, carried out serological tests and rapid diagnostics, Worked on BacT/ALERT®, Flow cytometry on BD® FACS Calibur also ran specialty panels on MicroScan®

MAXLABS (NABL certified) at MAX Super Specialty Hospital Saket, New Delhi (Intern) Jul-Aug’10

Learned to run important diagnostic tests on state-of-the-art equipments like Beckman Coulter UniCel® DxI 800 Immunoassay System, Performed automated ELISA on Dade Behring BEP III.

Achievements & Extra Curricular Activities :

• 1st prize in art and craft competition “Scintillate your Art” in National level techno-cultural fest “Excelsior 2008”

• Winner of clay modelling in National level technical and cultural fest organized by the college “Excelsior 2010”.

• Winner of Quiz and aptitude test organized by the institutes’ biotech club “Genesis”, 2008.