STEPHANIE M GLADDEN
*********.*.*******@*****.***
EDUCATION
Sacramento State University; Sacramento, CA
Bachelors of Science in Molecular Biology, May 2008.
Courses: Organic Chemistry, Molecular Biology, Microbiology, Medical Mycology, Genetics, Ecology, Biochemistry, Inorganic Quantitative Analysis, Cell Physiology, Immunology.
RELATED SKILLS
Analytical Chemistry
• Operates high-pressure liquid chromatography (HPLC), gas chromatography (GC), and atomic absorption spectrophotometry.
• Practices safety precautions in laboratory environment.
• Accurately weighs and measures powdered, liquid, and solid substances.
• Creates standards for inorganic (CaCO3, Co, Cr) and organic (naphthalene, benzene) analysis.
• Creates buffer solutions to ± 0.01 pH.
Molecular Biology
• Prepares DNA and RNA samples: centrifuging and analyzing for contaminants, purifying nucleic acids with lab kits (QIAGEN) and with home-grown lab protocols.
• Performs Northern and Southern blots with E. Coli samples.
• Sequences human and bacterial DNA with a chemi-luminescent sequencer (Applied Biosystems 310 DNA Analyzer).
• Clones E. Coli recombinant plasmids with antibiotic resistant genes (ampicillin and kanamycin)
• Transforms E. Coli with recombinant plasmids.
• Runs gels via electrophoresis (agarose and SDS).
• Safely worked with ethidium bromide to develop gels.
• Performs PCR reactions with human trace DNA and E. Coli DNA.
• Performs DNA paternity testing with blood and cheek swabs via STR analysis.
Biochemistry
• Protein purification with ion exchange column chromatography and size exclusion chromatography.
• Tested results with SDS-PAGE.
• Performed Western blots with self purified proteins.
Organization/Coordination
• Have completed formal laboratory reports outlining findings and operations of instruments used.
• Experience compiling data neatly and correctly in Excel spreadsheets, informal laboratory notebooks, and formal laboratory reports.
Professional Profile
• Able to handle multiple tasks under pressure and meet deadlines.
• Positive attitude and great interest in the fields of chemistry and biological sciences.
• Practices positive workplace methodologies such as 5S, 3R, and LEAN.
• Creates and performs troubleshooting procedures with ease.
• Efficient and capable of independent work.
WORK EXPERIENCE
Jun 1, 2010 - State Personnel Board of California
present Sacramento, CA 95814
Staff Services Analyst
• Review and determine outcome of servicewide exam requests from departments
• Review and test hotfixes from vendor for online examination and certification web application
• Assist public with applying to SPB examinations
• Maintain and troubleshooting online examinations with external and internal exam sources
• Function as technical support for Staff Service Analysts and Associate Personnel Analysts at SPB and external departments
Mar 16, 2008 – State Personnel Board of California via University Enterprises
May 31, 2010 Sacramento, CA 95814
35 hours/week Student Office Assistant
• Reviewed and tested full releases and hotfixes from vendor for online examination and certification web application
• Recorded errors from business and IT staff and relayed to vendor’s development team, functioning as the communication link between SPB business and vendor
• Developed use cases and test scripts for admin and public user suites
• Developed new functionality to the legacy system’s mechanism with various branches of SPB (IT, web team, and business) and the vendor
• Assisted general troubleshooting with public client side website and administration suite
• Designed, maintained, and executed test scripts using SilkTest 2008
• Worked with two Staff Information Systems Analysts to study data usage patterns, server capacities for load, and query databases for reports using SQL
• Assisted training sessions with exam analysts from state departments as technical and error support
2006 - 2007 California State University, Sacramento Sacramento, CA
Geography Department
Office Assistant
• Answered a multi-line telephone and assisted with call directory
• Aided professors with projects for specialized geography classes (climatology, California water systems, international topology)
• Processed, edited, and analyzed documents and class assignments for department chairman and professors
• Worked independently to assist professors with various department wide and individual projects
• Aided in process of interviewing and hiring faculty
• Managed travel arrangements and forwarding communications to department chairman
• Sorted mail
• Eased strain on secretary by providing support and assistance, sometimes through substitution
TECHNICAL SKILLS
Testing Software FogBugz, IBM Rational ClearQuest, SilkTest 2008, BugZilla
Platforms Windows 98/Me/XP/Vista/7
Languages Intermediate SQL, HTML, JavaScript, CSS
Software Microsoft Office Suite, MS Visio, MS SQL Server, Oracle SQL Developer
References available upon request.