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Lab Assistant/Lab Technician

Location:
Antelope, CA, 95843
Posted:
July 17, 2010

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Resume:

STEPHANIE M GLADDEN

916-***-****

*********.*.*******@*****.***

EDUCATION

Sacramento State University; Sacramento, CA

Bachelors of Science in Molecular Biology, May 2008.

Courses: Organic Chemistry, Molecular Biology, Microbiology, Medical Mycology, Genetics, Ecology, Biochemistry, Inorganic Quantitative Analysis, Cell Physiology, Immunology.

RELATED SKILLS

Analytical Chemistry

• Operates high-pressure liquid chromatography (HPLC), gas chromatography (GC), and atomic absorption spectrophotometry.

• Practices safety precautions in laboratory environment.

• Accurately weighs and measures powdered, liquid, and solid substances.

• Creates standards for inorganic (CaCO3, Co, Cr) and organic (naphthalene, benzene) analysis.

• Creates buffer solutions to ± 0.01 pH.

Molecular Biology

• Prepares DNA and RNA samples: centrifuging and analyzing for contaminants, purifying nucleic acids with lab kits (QIAGEN) and with home-grown lab protocols.

• Performs Northern and Southern blots with E. Coli samples.

• Sequences human and bacterial DNA with a chemi-luminescent sequencer (Applied Biosystems 310 DNA Analyzer).

• Clones E. Coli recombinant plasmids with antibiotic resistant genes (ampicillin and kanamycin)

• Transforms E. Coli with recombinant plasmids.

• Runs gels via electrophoresis (agarose and SDS).

• Safely worked with ethidium bromide to develop gels.

• Performs PCR reactions with human trace DNA and E. Coli DNA.

• Performs DNA paternity testing with blood and cheek swabs via STR analysis.

Biochemistry

• Protein purification with ion exchange column chromatography and size exclusion chromatography.

• Tested results with SDS-PAGE.

• Performed Western blots with self purified proteins.

Organization/Coordination

• Have completed formal laboratory reports outlining findings and operations of instruments used.

• Experience compiling data neatly and correctly in Excel spreadsheets, informal laboratory notebooks, and formal laboratory reports.

Professional Profile

• Able to handle multiple tasks under pressure and meet deadlines.

• Positive attitude and great interest in the fields of chemistry and biological sciences.

• Practices positive workplace methodologies such as 5S, 3R, and LEAN.

• Creates and performs troubleshooting procedures with ease.

• Efficient and capable of independent work.

WORK EXPERIENCE

Jun 1, 2010 - State Personnel Board of California

present Sacramento, CA 95814

Staff Services Analyst

• Review and determine outcome of servicewide exam requests from departments

• Review and test hotfixes from vendor for online examination and certification web application

• Assist public with applying to SPB examinations

• Maintain and troubleshooting online examinations with external and internal exam sources

• Function as technical support for Staff Service Analysts and Associate Personnel Analysts at SPB and external departments

Mar 16, 2008 – State Personnel Board of California via University Enterprises

May 31, 2010 Sacramento, CA 95814

35 hours/week Student Office Assistant

• Reviewed and tested full releases and hotfixes from vendor for online examination and certification web application

• Recorded errors from business and IT staff and relayed to vendor’s development team, functioning as the communication link between SPB business and vendor

• Developed use cases and test scripts for admin and public user suites

• Developed new functionality to the legacy system’s mechanism with various branches of SPB (IT, web team, and business) and the vendor

• Assisted general troubleshooting with public client side website and administration suite

• Designed, maintained, and executed test scripts using SilkTest 2008

• Worked with two Staff Information Systems Analysts to study data usage patterns, server capacities for load, and query databases for reports using SQL

• Assisted training sessions with exam analysts from state departments as technical and error support

2006 - 2007 California State University, Sacramento Sacramento, CA

Geography Department

Office Assistant

• Answered a multi-line telephone and assisted with call directory

• Aided professors with projects for specialized geography classes (climatology, California water systems, international topology)

• Processed, edited, and analyzed documents and class assignments for department chairman and professors

• Worked independently to assist professors with various department wide and individual projects

• Aided in process of interviewing and hiring faculty

• Managed travel arrangements and forwarding communications to department chairman

• Sorted mail

• Eased strain on secretary by providing support and assistance, sometimes through substitution

TECHNICAL SKILLS

Testing Software FogBugz, IBM Rational ClearQuest, SilkTest 2008, BugZilla

Platforms Windows 98/Me/XP/Vista/7

Languages Intermediate SQL, HTML, JavaScript, CSS

Software Microsoft Office Suite, MS Visio, MS SQL Server, Oracle SQL Developer

References available upon request.



Contact this candidate