Anatoly Mayburd CV

Anatoly Mayburd

**** ***** ***** **., ****, Alexandria, VA 22303, United States

Cellular 240-***-****

*******@*****.***

TECHNICAL AND IP RESEARCH

SUMMARY

• 13 + years of research in Molecular Biology, Bioinformatics and Chemical Engineering.

• First author of peer reviewed publications, pending patent applications.

• PhD in Molecular Biology, NIH/NCI post-doctorate, Bachelor in Chemical Engineering.

• Extensive and diverse technical background combined with technical and organizational creativity.

• Skilled in multiple tools of data mining, analysis and statistical/mathematical modeling, emergency situation modeling and response planning.

• Registered Patent Agent.

• US citizen, clearable.

• Knowledge of Russian language and culture, experience of working abroad.

• Performance-related bonuses, Certificates of Excellence issued by the current and past employers.

• DTRA Golden Star Award.

EXPERIENCE

Independent IP Contractor, Alexandria VA

5/2012 – present

• Landscaping analysis of technologies in medicine, biotech, chemical engineering, chemistry and formulations, nuclear engineering, medical devices, computational and business methods, material science and mechanical fields.

• Patent application preparation and prosecution.

• Perform Accelerated USPTO Examination searches, Freedom-to-Operate, State-of-the-Art, Infringement, Validity, Opposition, Patentability searches of prior art.

Landon-IP, Alexandria VA

Bilingual Patent Analyst, 1/2011 – 5/2012

• Landscaping analysis of technologies in medicine, biotech, chemical engineering, material science and mechanical fields.

• Conduct PCT Article 18 examination of international patent applications at the request of International Search Authority (ISA), establish International Search Reports and Written Opinions by PCT Article 43. The examination includes evaluation of the issues of patentability, invention description and claim support, new matter, utility, invention unity, formalities etc. Perform Accelerated USPTO Examination, Freedom-to-Operate, State-of-the-Art, Infringement, Validity, Opposition, Patentability searches of prior art.

• As a technical lead, maintain and advance scientific background by continuous scientific research in the fields of expertise. Filed a patent application US 13/067,798 on June 28, 2011, currently pending, titled “Methods of pulsed nuclear energy generation using piston-based systems”. Explored and published a treatise on a possibility of miniaturized fission-fusion hybrid reaction core with positive fusion yield in “Nuclear Engineering and Design”, Volume 241, Issue 1, January 2011, Pages 59-66.

• Patent application preparation.

• Participate in business development, estimate and proposal writing.

CPA Global, Alexandria VA

Senior Intellectual Property Researcher, 3/2007 - 1/2011

• Performed Accelerated USPTO Examination searches, Technological Landscaping, Freedom-to-Operate, State-of-the-Art, Infringement, Validity, Opposition, Patentability searches of prior art.

• The topics of research comprised medicine and medical devices, therapies, diagnostics, biology related computational methods, pharmaceutical compositions, stem cell methodologies, chemical processes, composite materials and organic synthesis, petrochemistry, separations and purification, mechanisms and machine parts, fuel and electrochemical cells, algorithms, business methods and more.

• In 2008-2010 developed a novel method of IP landscaping analysis, assessing a probability for a given technological sector to yield a block-buster commercial product. The topic of the landscape studies were countermeasures against pandemic influenza and small-pox. The method may be useful for placement of venture capital and approval of grant proposals. The method was published as invited reviews in PRI journal (see below the list of references). The invention was patented by the employer (see below).

• Patent application preparation.

• Was working on maintaining and advancing scientific background by continuous scientific research in the fields of expertise.

Northrop Grumman/ DTRA, Ft Belvoir, VA

Senior Reachback Engineer IV, 3/2006 - 3/2007

• Conducted writing of technical scenarios of potential WMD attacks and proposed countermeasures. The scenarios were well accepted by DTRA colleagues and by Civil Support Team personnel and were later used in the preparedness training.

• Conducted computational support of Army/National Guard WMD/natural disaster preparedness training, with the focus on chemical and biological warfare. Facilitated Army WMD preparedness training exercises by answering the technical questions of military personnel in peaceful and combat conditions.

• Conducted a live urgent analysis of real situations using encrypted lines, was awarded DTRA Golden Star.

• Performed additional duties as a substitute training program coordinator.

• Conducted a research on the possibility of creating supra-miniature hybrid fissile matrices, capable of supporting a controlled fusion component. The results were later published by the NED journal (Nuclear Engineering and Design). The material of the article was filed as a provisional patent application with USPTO in 2011.

• Conducted a research of existing countermeasures against pandemic infections and passed field training in identification of the pathogens by rapid PCR.

NIH/NCI, Bethesda, MD

Post-doctoral fellow, 9/2002 - 3/2006

• Designed and performed a study of lung cancer producing a computational method assessing a potential of a gene to express a successful anti-cancer drug target, resulting in 6-fold enrichment in FDA-approved anti-cancer targets at 50% recall rate. The method allowed 40-fold+ enrichment for anti-microbial targets at 45% recall rate as well. Translational applications based on gene scores produced by this method may facilitate development of new therapies. These studies led to a still broader research into the origin of chronic diseases and into general characteristics of a successful pharmacological target, using integrated datasets of multiple sources. Developed and employed customized microarray data processing plug-ins. The methods mentioned above were published in Bioinformatics Oxford, 2007 and in Plos One, 2009.

• Designed and conducted microarray study of MK886 drug effect upon lung cancer cell lines and transgenic mice. MK886 study resulted in a proposed novel mechanism of anti-cancer activity with high potential of developing synergistic and super-additive drug and agent combinations. The findings were published in Clinical Cancer Research, 2006 and Cancer Research, 2005 see below.

• Designed and conducted a study aiming at understanding of biological function of FLAP (5-lipoxygenase activator protein) and the pharmacological mechanism of its antagonist MK-886 in lung cancer. The protein is the upstream initiator of leucotriene biological pathway, important in cancer development, inflammation, allergies, asthma, autoimmune and aging diseases. Constructed cell lines with over-expressed FLAP protein level and demonstrated increased sensitivity to MK886. Additional role of FLAP as an apoptotic perinuclear trigger was proposed and presented at NIH Research Festival, 2004.

Rush University, Chicago, IL

Post-doctoral fellow, 07/2001 - 08/2002

• Designed and performed a study of conformational changes in viral fusion proteins of influenza HA and HIV gp41 classes supporting penetration of the cellular outer membrane by a viral particle. Understanding of their complex conformational dynamic is important for design of novel inhibitors.

• Proposed FRET-based assay of 6-helix bundle cleaving ligands, as potential novel sensitive screening method for anti-influenza drug candidates.

• Acquired valuable understanding of molecular basis of these two very important human pathogens.

University of Illinois at Chicago, Chicago, IL

Doctorate project, 1994 – 2001

• The project was focused on establishing of a biological function of a bacterial protein cytochrome c prime, found in a number of anaerobically growing pathogens.

• A number of research techniques such as oxygen-free gas phase titrations, stop-flow kinetics,

reconstitution experiments, binding constraint extraction, point mutagenesis and bioinformatic analysis allowed assignment of biological function to the protein as a nitric oxide shuttle.

• The results were published in Biochemistry, 2002.

• In the process of working with a gel marker dye BPB its high affinity binding to cytochrome C prime was discovered and Rasmol analysis of the protein X-ray crystallization data uncovered an extensive hydrophobic patch on its surface. BPB turned out to be a useful spectral probe for detection of active sites in enzymes and the results were published in Analytical Biochemistry, 2003.

SKILLS AND SOFTWARES

Methods and tools of Homeland security

• Integrated use of HPAC4.04R, CATS v.1 and v.6, ArcGIS9.1 and other specialized tools of consequence assessment in CBRNE analysis.

• Sample collection, preparation and field identification of biological warfare agents using glass capillary/rapid PCR, statistical assessment of the data and integration of the field results with analytical software output.

Technical information research

• Skilled in comprehensive research of technical literature by applying end-user (PatBase, EAST, Micropatent, Delphion, Thompson Innovation) and integrated databases, including chemical structure such as Questel, Merged Markush Service (MMS), TOPGRAG structure drawing interface, STN. Extract merger, acquisitions and new product announcements using DIALOG by Thomson.

• Conduct sequence searching using STN and GenomeQuest.

• Expertly leverage the resources of the Library of Congress and of the National Library of Medicine.

Molecular Biology

• NCI-60 panel screening.

• Real-time PCR.

• Flow cytometry and confocal microscopy, FRET fluorescent labeling technique, two-dye cell fusion assays, fluorescent microscopy, immunodetection (FACS, ELISA, immunoprecipitation, antibody labeling, Fab production).

• Standard culturing of primary and malignant cell lines.

• In-vitro reconstitution experiments.

• siRNA treatment of the drug's target protein's transcript.

• Affymetrix microarray primary analysis (MAS5, MadBee system), sharing with public domain via GEO uploads and downloads, data processing using Partek Pro and Madbee algorithms.

• Clustering, Principal Component Analysis and Classifier building).

• Point mutagenesis, and vector construction.

• Purification and screening of potential partners for a target protein utilizing volume exclusion, ion exchange, hydrophobic chromatography, affinity column construction, and iso-electric focusing, designed in-vitro reconstituted reporting system from purified bacterial components. Performed mobility shift assays in non-denaturing acrylamide and agarose gels.

• Yeast Two-hybrid system I for detection of the interaction partners of cytochrome c'; practiced culturing bacteria other than E. coli.

• Ligand binding and quick kinetic (stop flow) studies under both aerobic and anaerobic conditions, analysis of binding and kinetic data by SigmaPlot, fitting and extraction of affinity constants, and comparison of alternative binding models.

• RasMol, BLAST, VAST, PROPSEARCH, COG, and other bioinformatics tools.

Modeling skills

• Response surface construction and “black-box” optimization of experimental systems.

• Construction of prognostic classifiers.

• Data fitting, extraction of important parameters from linear and non-linear regression models.

• Modeling of emergency situations and approximation of the response effort using CBRNE tools (HPAC, CATS).

Teaching and training experience

• Taught General Chemistry 101, 112, 114, 116, Organic Chemistry 232, 234, Biochemistry 354, Physical Chemistry 223, 224, Independent research 450, both classes and labs.

TRAINING

Homeland security analytical methods

• "FIBWA- Field Identification of Biological Warfare Agents" at Ft. Detrick (11-12/2006).

• Computerized disaster zone analysis: HPAC4.04 level 1-3, ArcGIS9.1/CAT6.0 level 1.0 courses. (4/06).

Technical information research methods

• Training course on technical information research with the Current Employer (1/2011-2/2011).

• Patent Bar training course with the Current Employer (5/2011).

• On-line and hands-on training in PCT patent examination with the Current Employer (5/2011-8/2011).

• A training seminar "Advanced sequence searching using STN" (5/2010).

• Library of Congress and National Library of Medicine use training (7/2009).

• A training seminar "Research of European and International prior art" (3/2009).

• Attended a teaching PIUG conference "Sequence searching and Beyond", Cambridge, MA. (2/2009).

• Advanced Patbase, Thompson Innovation, Questel Patent training (11/15-17/2008).

• STN search training using Markush structure representation of chemicals, STN REGISTRY search to produce efficient queries, STN sequence searches (9/2008).

• Advanced Delphion and Micropatent training with Thompson (9/2008).

• DIALOG online training (3/2007).

• EAST user course (3/2007).

Molecular Biology research methods

• "Principles of Clinical Pharmacology" lecture series (Fall 2004-Spring 2005).

• NIH FAES, TRAC30, BIOTRAC course: "Small interfering RNA (siRNA) and Functional Genomics, theory and hands-on training" (Fall 2003).

• CIT: Partek Pro ~ Affymetrix data acquisition and quality assessment training (Spring 2003).

• NCBI: Locus Link Quick Start, MadBee system, Microarray analysis, Radiation, Occupational, and Computer Safety mini-courses (Fall 2002).

EDUCATIONAL BACKGROUND

Doctor of Philosophy, Molecular Biology, 2001

University of Illinois at Chicago, Chicago

GPA: 3.5/4

Bachelor of Science, Chemical Engineering with the focus in bioreactors, 1988

MHTI (RHTU), Moscow Chemical Technological Institute named after Mendeleev, Moscow

GPA: 4.6/5

AFFILIATIONS

PIUG (patent information users group), ACS (American Chemical Society), Review Board of Bioinformatics.

HONORS

DTRA Golden Star, 2006

Performance-related bonuses 2007-2010

Certificate of Excellence by CPA, 2008

Certificate of Excellence by the current employer, 2011

PATENTS

PCT/US10/58667 filed on December 1, 2010, currently pending, titled “Methods and systems for performing analysis on documents related to different technological fields”.

US 13/067,798 filed June 28, 2011, currently pending, titled “Methods of pulsed nuclear energy generation using piston-based systems”.

DHHS ref. no. E-268-2005/0-US-01, M&G ref. no 11613.76USP1

filed March 31, 2006, currently abandoned, titled “Methods and systems for identifying and classifying drug targets”.

PUBLICATIONS

1: Mayburd AL. Smallpox antiviral therapeutics. Recent Patent Antiinfect Drug Discovery 2011, Volume 5, number 4, pp 240-256, a solicited review.

2: Mayburd AL. A possibility of miniaturized fission-fusion hybrid reaction core with positive fusion yield. Nuclear Engineering and Design, Volume 241, Issue 1, January 2011, Pages 59-66.

3: Mayburd AL. Influenza antiviral therapeutics. Recent Pat Antiinfect Drug Discov. 2010 1;5(1):64-75, a solicited review.

4: Mayburd AL. Expression variation: its relevance to emergence of chronic disease and to therapy. PLoS One. 2009 Jun 15;4(6):e5921.

5: Mayburd AL, Golovchikova I, Mulshine JL. Successful anti-cancer drug targets able to pass FDA review demonstrate the identifiable signature distinct from the signatures of random genes and initially proposed targets. Bioinformatics. 2008 Feb 1;24(3):389-95. Epub 2007 Oct 8.

6: Mayburd AL, Martlínez A, Sackett D, Liu H, Shih J, Tauler J, Avis I, Mulshine JL. Ingenuity network-assisted transcription profiling: Identification of a new pharmacologic mechanism for MK886. Clin Cancer Res. 2006 Mar 15;12(6):1820-7.

7: Avis I, Martínez A, Tauler J, Zudaire E, Mayburd A, Abu-Ghazaleh R, Ondrey F, Mulshine JL. Inhibitors of the arachidonic acid pathway and peroxisome proliferator-activated receptor ligands have superadditive effects on lung cancer growth inhibition. Cancer Res. 2005 May 15;65(10):4181-90.

8: Bertsch M, Mayburd AL, Kassner RJ. The identification of hydrophobic sites on the surface of proteins using absorption difference spectroscopy of bromophenol blue. Anal Biochem. 2003 Feb 15;313(2):187-95.

9: Mayburd AL, Kassner RJ. Mechanism and biological role of nitric oxide binding to cytochrome c'. Biochemistry. 2002 Oct 1;41(39):11582-91.

10: Mayburd AL, Tan Y, Kassner RJ. Complex formation between Chromatium vinosum ferric cytochrome c' and bromophenol blue. Arch Biochem Biophys. 2000 Jun 1;378(1):40-4.

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