Safety Officer Sales

John F. Ksiazek

** ***** ***** ********, *********** 06712 203-***-****(Home) 203-***-****(Cell) ***********@*********.***

Objective

Energetic and productive research scientist with professional experience in the pharmaceutical industry seeking a new challenge to utilize diverse skill set.

Motivated to join a team focusing on drug discovery or development or biotech sales.

Key elements and assets: scientific innovation, productive collaborations, and adherence to industry regulation standards

Employment

Research Scientist 1979-2010

Boehringer Ingelheim Pharmaceuticals Incorporated

Ridgefield, Connecticut 06877

1. Development and validation of immunological assays for screening vital to project team goals and advancement

2. Proficiency in cell tissue culture, established cell lines, primary cell culture, cell transfection , human whole blood cell isolation

3.Effective and influential communication through presentations to core team and diverse leadership groups

4.Implementation of new technology ( flow cytometry) including troubleshooting, maintenance, and training for expanded utilization

5.Provided leadership as departmental safety officer; lab audits with recommendations and initiative to provide a "greener" lab environment

6. Involved in community :Partners in Science, Science Horizons Symposium Coordinator, Habitat for Humanity, Regional Hospice Art Show

Research Assistant 1974-1979

Department of Pathology

University of Connecticut Health Center

Farmington, Connecticut 06032

Academic research focused on the study of lymphokines, delayed hypersensitivity

1. In vivo several animal species used for immunizations, testing , and as sources of immune cells

2. Cell migration and Chemotaxis assays

3. Cell proliferation assays, antigen induced proliferation, MLR

4.Trained visiting researchers and UCHC graduate students basic techniques to assay cell mediated immunity

Technical Skills

1. Tissue culture: cell lines, primary cell culture, cell transfections

2. Cell proliferation assays, cell binding assays

3. Standard ELISA and MSD ELISA

4. Cell migration and chemotaxis assays

5. Flow Cytometry immuno-fluorescent staining and analysis

6. Cell transfections; electroporation

7. Gel Electrophoresis cell lysates protein gels, protein sizing

8. Western blotting; protein separation, antibody probes for detection and quantification

Education

Masters Health Science: Public Health Quinnipiac College Hamden, Connecticut

Bachelor of Arts: Biology, Pathobiology University of Connecticut Storrs, Connecticut

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