Achuta Apr 09
Resume:
Achuta Gonnabathula
Whitinsville, MA 01588
***********@*******.***
Summary
Biomedical researcher with experience in Quality Control, molecular and cell biology, seeking to apply technical and interpersonal skills to a new and exciting career.
Skills
Environmental monitoring, Bioburden, LAL, TOC & PH, sterility testing by membrane filtration, sterility testing by direct inoculation, growth promotion of media, Gram-staining, water analysis. Clean steam analysis, Compressed air monitoring, AQL inspections, GMP and GLP practices.
Pro- and eukaryotic aseptic cell culture techniques, SDS-Page and Western blot, immunohistochemistry, spectrophotometry, ELISA, photomicrography and film development, fluorescent microscopy and imaging, small animal handling for drug administration through IP.
MS Word, Excel, Adobe Photoshop.
Experience in Research & Quality Control
AstraZeneca, Westboro, MA Aug 2007 – Feb 2009
Microbiologist
Sterility Testing by direct inoculation and membrane filtration
TAC or Bioburden Testing of purified water, swabs, solutions and clean steam samples
BET Testing of purified waters and pretreatment waters
Particulate Testing of different products using HIAC machine
Inspection of Media fills
Use of LIMS (Lab Information Management System) for receiving and entering results for sterility testing and bioburden testing
Use of ELSM (Electronic Learning System Management) for SOPs
Entry of Data in AS400 system
Avant Immunotherapeutics, Fall River, MA Apr 2006 – Mar 2007
QC Analyst II - Microbiology
Performed environmental monitoring testing in class 100, 1000, 10000, and 100000 areas.
Revised SOPs, when necessary.
Identification of Organisms using Gram Stain.
Growth promotion on different types of media.
Water sampling and analysis (Bioburden, LAL, TOC).
Clean steam sampling and analysis.
Compressed air monitoring.
Performed biological indicator testing.
RenaMed Biologics, Lincoln, RI May 2005 – Nov 2005
QC Microbiologist II
Performed a wide variety of inspections, checks, tests, and sampling procedures for the manufacture of products.
Performed environmental monitoring testing in class 100, 10000, and 100000 areas.
Monitored critical equipment and instrumentation.
Compiled data for documentation of test procedures and prepared reports.
Reviewed data obtained for compliance to specifications.
Routinely used GMP and GLP practices.
Performed data review.
Updated and maintained trending files.
Routinely assisted and trained peer analysts.
KeraCure, Worcester, MA Sep 2004 – May 2005
Research Assistant
Maintained and cultured keratinocytes for KeraPac™ used for skin grafting. Determined cell titer and loaded onto Cytodex beads.
Performed Alamar Blue assay to determine metabolic activity of keratinocytes for shelf life studies.
Conducted environmental monitoring using RCS Strips and contact plates. Identified contaminating organisms.
Performed endotoxin tests and sterility tests on client’s samples.
Monitored and maintained equipment.
Bio test for growth promotion – This test is performed using Aspergillus niger, Bacillus anthracis and Candida albicans, to validate the quality of newly acquired media. Results documented following SOPs.
Tufts - New England Medical Center, Boston, MA Mar 2003 – Sep 2004
Research Assistant, Pulmonary and Critical Care Research Lab
Dr. Usamah Kayyali
Cultured different human cell lines (BPAEC, RPMEC, HMVEC, HUVEC, HPAEC, HSVEC, MEFMK2, MEFWT, and EAHy926 cells).
Coulter Counting – Counted for experiments on MEFs (Fibroblasts) to monitor the growth inhibiting effect of TGF-beta.
Performed Western blotting to evaluate expression of Map kinase pathway members in different cell lines.
Analyzed cytoskeletal changes in cells exposed to hypoxic or normoxic conditions by immunocytochemistry
Determined protein concentration by Bradford and BCA protein assays.
Dr. Alex White
Identified serum markers of oxidative stress in patients with bone marrow transplants by ELISA.
Tufts - New England Medical Center, Boston, MA Jan 2002 – Feb 2003
Research Assistant, Pulmonary and Critical Care Research Lab, Dr. Joe Lanzillo
B.J. Medical College, Ahmedabad, India 1996
Research, Dr. Rajan Rawal
Conducted therapeutic trials to evaluate the safety and efficacy of calcipotriol ointment in the treatment of chronic plaque psoriasis.
Education
MD in Dermatology
B.J. Medical College, Ahmedabad, India 1993- 1996
Dissertation on cutaneous manifestations of pregnancy
Diploma in Venereal Disease
B.J. Medical College, Ahmedabad, India 1995
Bachelor of Medicine and Bachelor of Surgery (MBBS)
B.J. Medical College, Ahmedabad, India 1986- 1991
Publications
An SS, Pennella CM, Gonnabathula A, Chen J, Wang N, Gaestel M, Hassoun PM, Fredberg JJ, Kayyali US. Hypoxia alters biophysical properties of endothelial cells via p38 MAPK- and Rho kinase-dependent pathways. Am J Physiol Cell Physiol. 2005 Sep;289(3):C521-30
Other
Green card holder – able to work for any employer in USA
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