Safety Officer Pvt Ltd
Resume:
Dear Sir/Madam,
I am Dr.Parthasarathi Reddy.T, with 9+ years experience in the assay development area with special emphasis on Receptor Radio Ligand binding, Tissue Culture, Enzyme assays, Molecular Biology and Cell based functional assays for G-protein coupled receptors. I have experience screening new chemical entities (NCEs) for receptor ligand binding activity and generation of stable cell lines for various G-Protein coupled receptors. I would like to humbly confess that I have successfully initiated the Radioactivity lab (including, permissions and formalities) and established Radio receptor ligand binding assays (Scintillation proximity assay technology and Liquid scintillation counting) for screening the NCEs for G-protein coupled receptors (serotonin) in Magene Life Sciences pvt Ltd, Hyderabad as well as in GVK Biosciences Pvt Ltd for 30 various GPCRs types and Previously I had worked with Suven Life Sciences Ltd, Hyderabad in the same field of Radio ligand binding studies for GPCRs on Central Nervous System (CNS) disorders. At Present I am working in Aurigene Discovery technologies, Hyderabad on Oncology, Inflammation & Anti-Infectives disorders especially on Macromolecular labeling assays and previously worked in Orchid Research Laboratories limited, Chennai, on metabolic disorders.
I am a self-motivated, diligent individual, committed to achieving high standards and professionalism in whatever I do. I would like to join in your esteemed research group, if given an opportunity; I assure you that i shall discharge my abilities and duties at my level best.
I hereby attach my Curriculum Vitae in support of my application. I trust that my application will receive your favorable consideration and look forward to hear from you.
Thanking you in anticipation,
Sincerely,
Dr T. PARTHASARATHI REDDY
RESUME
Dr. PARTHA SARATHI REDDY.T
Phone: +91 40 - 961-***-****
Lakshmi Nivas
Plot no: 202,
Flat no: 302, S.P.Nagar
Kukatpally, Near JNTU
Hyderabad-500072
A.P,India.
E-mail: *************@*****.***
PERSONAL DETAILS
Date of Birth: 05/July/1978
Sex: Male
Marital Status: Married
Nationality: Indian
ACADEMIC QUALIFICATIONS
Ph.d in Genetics, Osmania University, Hyderabad, India, Dec 2006 to Dec2011.
Master of Science (M.Sc) in Biochemistry, Bharthidasan University, Trichy, India.
Bachelor of Science (B.Sc), Sri Venkateswara University, Tirupati, India.
ACADEMIC EXPERIENCE
Title of Ph.D Topic:
A study on Molecular, Biochemical and inflammatory markers in myocardial infarction.
PROFESSIONAL EXPERIENCE:
1. At present working as a Scientist at Aurigene Discovery Technologies (Dr.Reddy,s Subsidiary) Limited, Hyderabad (Dec2009 to till present).
Nature of work:
Played a lead role in the establishment of Pyruvate Kinase M2
(PKM2) biochemical assay for PKM2 activators using human recombinant
enzyme for the Oncology Program.
Played a lead role in the establishment of microsomal Prostaglandin E
Synthase-1(mPGES-1) biochemical assay using Human & Rat enzyme for
Inflamation & Oncology Program.
Played a lead role in the development and standardization of Macromolecular labeling assays using six different radioligands (list shown below) with E.coli bacteria to check the mechansin of action of drug (MoA) using Top count NXT Scintillation Counter (Perkin-Elmer) for one of our client Program.-Antiinfective Program.
1. Thymidine [2-14C] 2. Uridine [5,6-3H]
3. Galactose, D [1-14C] 4. Glycerol [2-3H],
5. Leucine, L-[4, 5-3H(N)] 6. 2,6-Diaminopimelic acid [2,6-3H]
Played a lead role in the development and standardization of Secondary screening assays (list Shown below) for one of the anti infective program.
a) Haemolysis assay.
b) Bacterial membrane disruption assay by ATP leakage - S. aureus ATCC-29213, P. aeruginosa ATCC-27853 and E. coli ATCC-25922 or ATCC-47076.
c) Cytotoxicity assay in cell lines- K562, Hep G2 and HEK293.
Immmunophenotyping technique by Flow Cytometry (BD FACS CALIBUR)
Established degranulation assay from RBL2H3 cells using fluorescence method.
Established Nitric Oxide Synthase assay using RAW264.5 cells.
2. Worked as a Senior Research Associate in New Discovery Centre (NDD),
Invitro Biology Department, Orchid Research Laboratories Limited (ORLL),
Chennai (Oct 2008 to Dec2009).
Nature of work:
Worked on Metabolic disorders Area
Established the cAMP functional Cell Based assay using HIT-T15 Cells for Screening the NCEs against one of the G-Protein Coupled Receptors (GPCR) which is to be involved in Diabetes & Obesity.
Experience on insulin release assay using HIT-T15 Cells.
Carry out the Invitro Adipogenesis assay using 3T3-L1 cells.
Experience on glucose uptake assay using C2C12 myotubes.
Experience on human Ether-à-go-go (HERG) assay.
Maintenance and preservation of Various Cell lines such as HIT-T15, 3T3-L1, C2C12, HepG2 cells.
Carryout the Glycosylated Hemoglobin (Hb1Ac) assay to estimate the heamoglobin from animal blood.
3. Worked as a Research Scholar completely full time on my Ph.d topic (Dec 18, 2007 to Oct 2008).
4. Senior Research Associate, Biology Division, GVK Biosciences Private Limited, Hyderabad, India (June, 2005 to Dec17, 2007).
Nature of Work:
Played a Vital role in the establishment of radioisotope laboratory by getting the approvals from AERB-BARC, Mumbai and DGCA, New Delhi for the import of radio labeled materials.
Played a lead role in the development and standardization of receptor radio-ligand binding experiments for 30 different receptors (list shown below), using Top count NXT Scintillation Counter (Perkin-Elmer) by Filtration high throughput assay for screening New Chemical Entities (NCE).
a. Serotonin Group: 5-HT1A, 5-HT2A, 5-HT2C, 5-HT3, 5-HT6, and 5-HT7 Muscarinic Group: M1, M2, M3, M4 and M5
b. Opioid Group: μ, δ, and Kappa
c. Adenosine Group: A1, A2a, and A3
d. Dopamine Group: D1, D2L, D3, and D4.4 Histamine Group: H1 and H2
e. Adrenergic Group: α2A, α 2B, α 2C, β1, and β2
f. Transporter Assays: 5-HT, Nor epinephrine and Dopamine transporters.
Worked on various Nuclear Hormone Receptors Such as LXR, MR, TR & VDR receptors using radioactive methods including Liquid Scintillation Counting, Scintillation Proximity Assay (SPA), Charcoal Method for screening the New Chemical Entities (NCE) using Perkin-Elmer Micro beta liquid scintillation Counter and Perkin-Elmer Envision.
Also designed a template for data analysis of receptor binding assays. This template integrates the Graph-Pad software for the data analysis of saturation and competition binding curves pertinent to receptor-ligand assays by direct import of the data from Top Count NXT instrument without any manual data analysis. This template comprises of the Calculation of Z factor and Grub’s test.
Worked as a Radiological Safety Officer (RSO).
Maintenance of Radioisotope lab and disposal of radioactive waste as per the AERB (BARC) Guidelines.
Worked on intracellular calcium measurement assay (fluorescent method) for cell-based GPCR functional studies.
Involved in the generation of Stable cells for various Serotonin receptors (5HT3, M3, and H1).
Screening and characterization of Various GPCR expressed stable cell lines using binding assays
Isolation of membrane protein from transfected stable cells and carryout the protein estimation using BCA method.
Carryout the whole cell-binding assay for transient transfection cells.
Membrane preparation from rat brain tissues for carrying out the binding assays for various serotonin receptors.
Establishment of binding assays using native tissues for Opioid and Muscarinic receptors
Maintenance and preservation of transformed mammalian cell lines (HEK293, CHO-K1) for cell-based assays.
5. Research Associate in Genetic Engineering Department, Magene Life Sciences Private Limited, Hyderabad, India (Aug 09-04 to June 08-2005).
Nature of Work:
Played a lead role in the establishment of receptor radio-ligand binding Studies
for serotonin (5HT6) receptor.
Involved as a team member in the cDNA cloning and generation of stable cell-
lines (HEK293) over-expresses the serotonin (5HT6) receptor.
Worked on Glucose uptake assay using C2C12 & 3T3-L1 Cells.
6. Research Assistant in Discovery Biology Division, Suven Life Sciences Limited, Hyderabad, India (Feb 06-03 to Jun 19-04).
Nature of Work:
Worked on Central Nerve System Disorders
Performed a crucial role in the optimization of receptor radioligand binding studies for screening the New Chemical Entities (NCEs) against 5HT6, 5HT2c & 5HT3.
Involved in the standardization of scintillation proximity assay (SPA) for different Serotonin receptors on HIDEX plate chameleon TM LSC.
Involved in the development and standardization of Enzyme assay for MMP3 & Urokinase in high throughput assay for screening New Chemical Entities (NCE) using Perkin Elmer Envision.
Performed the general biochemical estimations from animal serum samples in high though-put format (96 well).
Instruments Handled
• Topcount NXT Scintillation Counter
• Immmunophenotyping technique by Flow Cytometry (BD FACS CALIBUR)
• Molecular device SpectraMax and Gemini XS FL Reader
• Hidex Plate Chameleon
• Perkin-Elmer –Flex drop
• Multi Drop (Thermo Fisher)
• ENVISION Multi plate reader
• PRECISION (Biotek)
• Cybio –Disc& Cybio Dilus liquod handling systems.
• Micro Beta TRILUX (Perkin Elmer) – Scintillation Counter
• Polar star BMG
• Tomtec Cell Harvester
• Perkin-Elmer Victor
Papers :
Association between apolipoprotein E gene polymorphism and myocardial infarction.
Parthasarathireddy Tanguturi, Bhoomireddy Pullareddy, Sampath Kumar P, Dwarkanath K. Murthy. Biochemical Genetics (Accepted- Find Attached Mail-Editor, Biochemical Genetics).
Parthasarathireddy Tanguturi 1, Bhoomireddy Pullareddy 2, Rama Krishna B.S3, Dwarkanath K Murthy1*Association of Lipoprotein Lipase HindIII Polymorphism with Myocardial Infarction in South Indian Population. Indian Heart Journal - Ms. Ref. No.: IHJ-D-12-00196 (Communicated).
ATTENDED CONFERENCES
• The Ramanbhai Foundation 5th international symposium: Advances in
Translational Research & Development
Themes: New Drug Discovery Research & Development in Cancer,
Inflammation, Cardio-metabolic desease.February 1-4, 2011.
• International symposium on Frontiers in genetics and biotechnology, retrospect& prospect 8th - 10th Jan 2006 Osmania University, Hyderabad.
• National conference on GENOMICS: IMPACT ON HUMAN HEALTH 2nd & 3rd FEBRUARY, 2007, Osmania University, Hyderabad.
• Seminar on Climate change and its impact on human health on 5th June, 2009 at Institute of Genetics, Begum pet, Hyderabad
SHORT TERM TRAINING
• Two weeks training at Montreal (Canada) for receptor binding studies on GPCR using Top-count NXT scintillation Counter, sponsored by Perkin-Elmer Life Sciences, Canada.
• Three days training on liquid handling system for HTS studies, sponsored by Beckmann Coulter, London, UK.
• Undergone Radiological Safety Officer (RSO) Training Program at Baba Atomic Research centre, Mumbai and Certified as a Qualified RSO through written exam conducted by BARC.
REFERENCE
1. Prof.D.K.Murthy Ph.D
Ex Head, Genetics Department
Osmania University
Hyderabad-560 004.
India.
Phone: 040-********
Email: ************@*****.**.**
2. Dr.A.Veera Reddy, Ph.d
Vice President, R&D
Suvenlifesciences Limited
Hyderabad- 500064
Mobile: +91-939*******
Email: *******@*****.***
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