Quality Control Software

EDUCATION

Master of Science in Biomedical Engineering, Sept 2009 to Jun 2011

Drexel University - Philadelphia, PA

B.E. in Biotechnology

Jaypee Institute of Information Technology University,Sept 2005-May 2009

CORE COMPETENCIES

Protein expression, extraction and purification

Immunology (Immunoassays: ELISA, ECLIA) toxicology and immunogenicity studies

ELISA, Bio analysis drug treated samples Protein production and Quality Control

HPLC – LCMS

GLP and GMP practices

DNA Sequencing

Liquid Robotics handler (Plate washers for ELISA and Luminex)

Sample preparation and analysis

WORK EXPERIENCE

Associate Scientist on Co-op, Immunotoxicology Lab, Sept 2011- Mar 2012

Glaxo SmithKline Inc.

Safety Assessment, Platform Science and Technology

Project: Screening if multiple pre-clinical RNA based drug candidate molecules for immunostimulatory potential.

• Whole blood stimulation

• Setting up in vitro whole blood and lymphocyte subpopulations for immunostimulation experiments with drug candidates for Duchenne Muscular Dystrophy.

• Harvesting the supernatants, treating them with drug and positive controls at various concentrations.

• Luminex

• Evaluating the cytokine levels and quantitatively determining sTNFR-1, IFN-α, IL-8, IL-6 and MCP-1.

• Human and Non-Human Primate Millipore Multiplex magnetic bead based immunoassay kits were used for sample processing and principles of flow cytometry were followed for the Luminex 100/200.

• Analyzing the data using xPONENT software and graphing it using Graph Pad Prism software.

• ELISA

• Automated Bioanalysis of chemokine levels (complement C3a and C5a) of the drug treated samples, followed by plate reading.

• Meso Scale Discovery platforms

• GLP validation of pharmacodynamic assays.

• Developing a MSD based protocol for monitoring myostatin levels in rat and monkey plasma for Multiple Sclerosis.

• Quality control

• Performing necessary quality control experiments on commercial products as per QC protocols and

Procedures.

• Cell separation using biotin labeled kits and flow cytometry.

• Separating T-cells from mouse liver and thymus using EasySep protocol and counting them.

• Preparing and analyzing the tissue samples from mouse after necropsy for flow cytometry.

• Analyzing the data and presenting them at departmental meetings and teleconferences.

• ECLIA experiments for validation of free myostatin testing in rat/monkey plasma.

• Analysis of effect of drug compounds on cytokine induction, tissue processing, GraphPad Prism for data interpretation, sample preparation and GLP training.

• Expertise in Liquid Handlers robots like Plate washers for ELISA, Luminex

Associate Scientist on Contract, Jun 2011 - Sept 2011

Glaxo SmithKline Inc. - Collegeville, PA

• Producing high quality DNA sequences from samples submitted by customers.

• Using AMPURE, SPRI based PCR clean up system to purify amplicons.

• Resolving the samples after PCR on capillary electrophoresis system, ABI 3730xl.

• Using Affymetrix gene chips to identify SNP genotypes.

• Proof reading the results and uploading the data for customer review using Nautilis software.

• Using Sanger Sequencing to provide remarkable results to customers in 1-2 business days.

Research Assistant

Chemical and Bimolecular Engineering Department, Jun 2010- Jun 2011

University of Pennsylvania

Master’s Thesis Project: Proteomic scale effect of HSP90 inhibition with 17-AAG and Radicicol

• Studied the effect of inhibiting heat shock proteins with Phase III clinical trial drugs.

• Performed SDS PAGE, Western Blotting and ELISA on human lung cancer cells and Mesenchymal stem cells after 24 hour drug treatment.

• Tryptic digestion of kinetically labeled A549 cells to perform Bioanalysis (peptide analysis) for drug treated cells using HPLC-LCMS.

• Studied the morphology and viability of cells before and after drug treatment using Phase contrast inverted microscopy.

• Performed statistical data analysis using Graph Pad Prism and SPSS and ImageJ.

Skills

Protein expression and purification, protein estimation, immunology, quality control and DNA sequencing

Awards

Dean's Fellowship Award, September 2009

A Drexel Dean's Award is a merit-based award for academic achievement. The award is bestowed upon

Full-time graduate students who are identified as outstanding applicants.

Groups

Member, Drexel Biomedical Graduate Student Association

Member, Drexel Graduate Women in Science and Engineering

LABOROTORY/TECHNICAL SKILLS

Wet lab skills:

•Protein detection techniques - SDS page gels, Coomasie/Ag/Ponceau staining, western blotting

• Protein estimation techniques - BCA, Bradford assays, spectrophotometry

• Dialysis of recombinant proteins, ultrafiltration techniques

• Pouring/casting handmade polyacrylamide gels for SDS page electrophoresis

• Making required buffers (stock and dilutions).

• GLP training, lab notebook maintenance

• Animal handling, necropsy and tissue preparation.

Equipment:

Luminex, Meso Scale Discovery platform, Bioplex, ABI 3730xl, 454, Affymetrix, Olympus IX-71 Phase Contrast Inverted Microscope, protein purification systems, LCMS [ESI-TOF],

Spectrophotometer, Spectrofluorometer, Nanodrop,

Sonicator, Odyssey Scanner, Common lab equipment (centrifuges, emulsiflex, incubators, autoclave,

Lyophilizer, pH meter etc).

Computer skills:

Graphpad prism, Microsoft Excel, Word, all equipment related software (UNICORN for HPLC,

Quantitative analysis software for LCMS).

Contact this candidate