Scientist / Manager Scientist
Resume:
Thuan Q. Trinh, Ph.D.
*** ***** *****, ************, ** 20877
301-***-****. ***********@*****.***
http://www.linkedin.com/pub/q-thuan-trinh/28/84b/4b5
SUMMARY
Productive, talented cell and molecular biologist with years of experience in pharmaceutical/ biotechnology industry. Demonstrated project achievement. Project initiative to products, S.O.P documentation, and publication. Diligent problem solver with extensive experience in molecular biology techniques, and technology. From E. coli competent development to mammary cell stable expression, recombinant protein, assay screening. E. coli genetic engineering to expression vector constructions. From nucleic acid purification to functional expressive cDNA library construction, screening, analysis. From phage display to lentiviral expression; RACE cloning to real-time PCR,QPCR.
PROFESSIONAL EXPERIENCE
Pharmaceutical R&D, Senior Scientist III then IV
Wellstat Therapeutics, Gaithersburg, Maryland 10/2001-12/2010
Project:
• Single chain hybrid hIL7-bHGF fusokin
• Single chain hybrid hIL7-951, hIL7-951R, 951-hIL7
PN951, a proprietary peptide that regulates the cell cycle of stem cells
Achievement:
• Patent application: Novel fusion proteins for regulation of hematopoietic stem cells.
• Presentation: Developing optimal protein expression vectors / CHOK1 suspension expression.
Previous Projects 1:
• Hyaluronic acid (HA) activated antibody
Achievement:
• Presentation: Clone and sequence the variable regions of the IgG antibody, IgGhv and IgKlv, using GSP and mRNA 5’RACE cloning.
Previous Projects 2:
• Pn2034, oral insulin sensitizer for the treatment of Type II diabetes
Achievement:
• Presentation: Gene Expression from ob/ob Mouse’s WAT and 3T3L1 adipocytes, Pn2034 vs. Rosiglitazone: Using Real-time PCR and Subtraction cDNA library constructed with ob/ob mice adipose tissue
Other Work:
• Wnt/ß Catenin directed Tcf driving expression in Lentivirus.
• Study EML-C1 receptor that binds to a proprietary peptide
• Phage display library.
• Two hybrids system: def-PPAR? SAR
• Promoter study: Pck1, Pde3b
• Gene expression: Micro-arrays, Luciferase, GFP, Northern blot, Real time PCR, and Nuclear Protection Assay.
In-vitro: CHOK1, HEK293, HepG2, H4IIe, 3T3L1 adipocytes…
In-vivo: ob/ob, db/db mice
Life Sciences / Biotechnology R & D Staff Scientist, then Senior Scientist
Gibco-BRL Life Technologies Incorporation
Rockville, Maryland 9/1992-10/2001
Achievements:
• Presentations:
STBL2TM: An Escherichia coli strain for the stable propagation of retroviral clones and direct repeat sequences.
Nucleic Acid Isolation with Silica Membrane and Magnetic Beads.
Micro scale cDNA library construction.
Paper-Based Archiving of Mammalian and Plant Samples for RNA
• SOPs and Trade Secret Documents:
Production of Max Efficient STBL2TM Competent Cell.
Production of Lyophilizing Competent Cells
Qualification and production of Northern blot.
Max excision of lambda cDNA libraries.
Large scale electro-poration of competent cells for cDNA library construction.
Isolation of total RNA from 5 mg to 1 g of animal tissues, or human biopsy samples.
Isolation of total RNA using cartridge.
Isolation of genomic DNA using cartridge.
Direct isolation of mRNA from culture cells and animal tissue using magnetic beads.
Isolation of mRNA with bead technology: Streptavidin beads and oligodT beads.
Micro scale cDNA library construction.
Full length cDNA library construction using plasmid vector.
Sizing cDNA libraries with PCR.
• Research Products:
Lyophilizing Competent Cells
Gene trapper (support protocol)
cDNA libraries, and custom cDNA libraries.
Northern blots.
Micro to Midi Total RNA Purification System: A System for a Wide Range of
Sample Types and Amounts.
Genomic DNA and total RNA Purification System using Single Tube.
Direct mRNA Purification System.
Nano Scale Direct mRNA Purification System.
DNA Precipitation System without using TCA.
FTA, Paper-Based Archiving of Mammalian and Plant’s DNA and RNA Samples.
• Patent and Patent Applications:
Methods for Lyophilizing Competent Cells.
Patent number: 6,960,464
Method for Synthesizing a Nucleic Acid using a Ribonuclease.
Thuan Q. Trinh and Chris Gruber
Pub. App. # 200********
Improved cDNA Synthesis.
Thuan Q. Trinh, Chris Gruber, Joel Jessee, and Fred Bloom.
Filed with the U.S. Patent and Trademarks Office on June 10,1998.
Methods and Compositions for Isolation of Biological Macromolecules
Domenica Simms and Thuan Q. Trinh
Pub. App. # 200********
Post doctoral Research fellow
Merck Sharp and Dohme Research Laboratory
Rahway, New Jersey 1/1991 - 9/1992
“Cis-Acting Sequences in the Upstream Region of the Human Glut-4 Gene respond to Insulin"
Thuan Q. Trinh, Marie Alexandria and Joel P. Berger. Unpublished research data.
EDUCATION
Ph.D. University of Cincinnati, College of Medicine,
Department of Molecular Genetics, Microbiology and Biochemistry
Cincinnati, Ohio USA
B.A. Temple University, College of Arts and Sciences
Philadelphia, Pennsylvania USA
PROFESSIONAL PUBLICATIONS
"The Role of DNA Secondary Structure in Deletion and Duplication between Direct Repeats in Escherichia coli; Differential Mutagenesis between the Leading and the Lagging Strand." Ph.D. dissertation (1990), University of Cincinnati College of Medicine.
"Preferential DNA Secondary Structure Mutagenesis in the Lagging Strand of Replication in E. coli".
Thuan Q. Trinh and Richard R. Sinden, Nature 352, 544-7 (1991).
"Preferential DNA Secondary Structure Mutagenesis in the Lagging Strand of Replication,"
Thuan Q. Trinh and Richard R. Sinden, Abstract for the 1992 ASBMB/Biophysical Society Meeting, The FASEB Journal, 6, A-150 (1992).
"The Influence of Primary and Secondary DNA Structure in Deletion and Duplication Between Direct Repeats in Escherichia coli".
Thuan Q. Trinh and Richard R. Sinden, Genetics 134, 409-422 (1993).
STBL2TM: An Escherichia coli strain for the stable propagation of retroviral clones and direct repeat sequences.
Thuan Q. Trinh, Joel Jessee, Fred Bloom and Vanessa Hirsch (1994), Focus 16:2.
An Escherichia coli strain for the stable propagation of direct repeat sequences and retroviral clones.
Thuan Q. Trinh, Fred Bloom and Vanessa Hirsch, Abstract for the 1994 Wind River Conference on Prokaryotic Biology.
"Differential DNA secondary structure mediated deletion mutation in leading and lagging strands".
William R. Koche, Thuan Q. Trinh (co-first authors) and Richard R. Sinden, Journal of Bacteriology 177, 4385-4391 (1995).
"Leading Strand Specific Spontaneous Mutation Corrects a Quasi-Palindrome by an Intermolecular Strand Switch Mechanism".
Thuan Q. Trinh, William R. Koche (co-first authors) and Richard R. Sinden, Journal of Molecular Biology 269, 176-187 (1997).
“Human RNA Products that Facilitate Gene Expression Studies”
Thuan Q. Trinh
Focus 22, No 3 (2000).
“Paper-Based Archiving of Mammalian and Plant Samples for RNA”
Natarajan, P.; Trinh, Q T.; Mertz, L.; Goldsborough, M.; Fox, D.K. BioTechniques 29: 1328-1333 (2000)
“Micro to Midi Total RNA Purification System: A System for a Wide Range of
Sample Types and Amounts"
Domenica Simms and Thuan Q. Trinh, Focus 24.2:21-23 (2002)
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