Training Maintenance

Senthil Nathan.S

A-***, T.N.H.B, Phase-II, Arcot- 632 506,

District: Vellore, Tamilnadu, India

Cell +91-989*******;

Email01: *****************@*****.***;

Email02: **************@*****.**.**.

Profile Snapshot: A Life science professional, working as a core facility supervisor in a Stem cell research institute, having experience in flow cytometry and imaging (fluorescent microscopy).

Objective: Growth in Cell and Molecular Biology research projects which involves sorting and imaging roles.

Professional Competencies: Flow Cytometry analysis and sorting ~ Imaging (fluorescent microscope) ~ Lab supervising ~ Managing a team ~ Gas chromatography ~ HPLC ~ AAS.

Career Summary

Centre for Stem cell Research, CMC Campus, Bagayam. (December 2008 to present)

Supervisor core facility

Duties include:

• Flow Cytometry analysis using BD FACS Calibur.

(Training technical staffs, Maintenance and trouble shooting for the same.)

• Flow Cytometry sorting using BD FACS Aria - 1.

(Training technical staffs, Maintenance and trouble shooting for the same.)

• Data analysis using Flow Jo / FACS Diva / Cell Quest pro / Venturi one.

• Supervising cGMP facility in the centre.

o Culture of large scale mesenchymal stem cells from human placenta.

o Culture of large scale mesenchymal stem cells from human bone marrow sample.

• Conducted workshop on Isolation, culture and characterization of adult stem cells.

• Imaging of the samples using Fluorescent Microscope. (Leica - DMI6000B).

• Team Management: Supervising Core Lab and cGMP technical staffs.

• Maintaining service logs, making service contract for all the instruments.

• Imaging of the histopathology slides using Leica DMI1000 with DFC290 camera.

• Understanding the researcher’s samples and supporting technically in designing the experiment/making antibody panel for flowcytometry and microscopy techniques.

• Budgeting for the Maintenance Contract and spare replacement for all the instruments.

• Maintenance of all the instruments in the centre.

• Managing, budgeting and stocking of the consumables in the stores.

Independent scientific project:

• Culture of mesenchymal stem cells from human placenta.

• Culture of mesenchymal stem cells from human bone marrow sample.

• Differentiation assay for MSCs from all the sources.

• Culturing of MSCs in cGMP area.

• Culture of mesenchymal stem cells from human bone marrow in a biological scaffold.

• Culture of Endothelial Progenitor cells.

• Culture of cell lines like CHO, K562, etc.

D1oils India Coimbatore (U.K.based,) (July 2005 to December 2008)

Junior Scientist (Department of Bio Chemistry)

Duties Include:

• Managed Quality lab, testing of Biodiesel and oil quality parameters and report submission.

• Supervision of 3 technicians working in the same lab.

• Budgeting, ordering and stocking of the required chemicals for the lab.

• Maintenance of the instruments and training the technicians to operate the same.

Worked in D1 oils Plc, U.K, R&D laboratory for 2 months (30/07/06 - 30/09/06)

Analyzed Biodiesel samples (both Physical and Chemical parameters) and trained plant operators to do some basic analytical tests.

Education

• MBA in Alagappa University, Directorate of Distance Education (2007 - 2009)

• M.Phil in Bio Technology, Bharathidasan University, (Distance Education) Thiruchi (2006 - 2007)

• Post graduate in Bio chemistry at S.R.M. College of arts and Science College, Chennai. 2005.

• Under graduate in Biochemistry with first class at AdhiParasakthi College of science, Kalavai. 2003.

Trainings

• Participant of 11th Indo –US cytometry workshop held at NCBS, Bangalore.

• Advanced flow cytometry course on cell sorting organized by “BD-NCBS Centre of excellence in flow cytometry at NCBS, Bangalore from 24/03/09 to 27/03/09. (Instructor: Dr. Krishnamoorthy, NCBS and Dr. PareshJain, BD).

• Basic course on Digital flow cytometry training organized by “BD-NCBS Centre of excellence in flow cytometry at NCBS, Bangalore from 20/01/09 to 23/01/09. (Instructor: Dr. Krishnamoorthy, NCBS and Dr. PareshJain, BD).

• “Live cell imaging “- an Internal workshop conducted in CSCR on March 2009. With the following course content. (Instructor: Dr. Prasad Karnik).

• Preliminary confocal imaging training with the following course content at Nomic Facility in NCBS, Bangalore. (Instructor: Mr. Prasad, NCBS).

• Training on Gas Chromatography, at Dr. Cee Lee Analytical Lab, at Metha College of Pharmacy, Thiruvanmiur, Chennai.

• Analytical workshop on “Introduction to Analytical Chemistry” held at Salim Ali Ornithology and Environmental Research Center in Annaikatti in Coimbatore.

• Discussion about Oil Analysis by using Gas Chromatography at IIP, Dehradun with Dr.A.K.Gupta and Dr.Bhatnakar.

• Training on Refinery plant, Bio Diesel production, and Oil Analysis by using Gas Chromatography at D1 Oils, R & D Lab. In Middlesborough UK.

• Short course on how to Develop, Validate and Trouble shoot GC methods Organized by American Chemical Society, held at National Chemical Laboratory, Pune (November 18th 2002).

• Training on Clinical laboratory techniques (Biochemistry, Microbiology, Bio-technology, and Histopathology) held at Schieffelin Leprosy Research & Training Center, Karigiri, and (May 31st 2004 to June 5th 2004).

• Training on Animal Tissue Culture Techniques, Egg inoculation, and ELISA held at Institute of Veterinary Preventive Medicine, Ranipet (December 25th 2004 to January 10th 2005).

• Training on Metals analysis using AAS (Atomic Absorption Spectroscopy).

Seminars & Conferences

• First International CMC Winter Symposium on Cell Biology and Molecular Medicine held at the Christian medical College Vellore on 30 and 31st Jan 2003.

• State level seminar on SanBiochemfest held at Sri Sankara arts and science college Enathur, Kancheepuram on 22nd to 24th Jan 2002.

• State Level Seminar on Science Fest 2003 held at D.K.M College for Women Vellore on 11th to 14th Feb. 2003.

Personal Profile

• Date of Birth: 30th June 1983.

• Languages Known: Tamil, English.

• Computer Proficiency: Oracle, VB, UNIX, C, C++, Windows, MS-Office).

• Interests: Sports: First Dan Black Belt in Karate (Japan Shito Ryu). and Basket Ball.

References

1. Prithi Rajan, Ph.D.

Associate Professor,

Centre for stem cell research,

Bagayam, Vellore – 2.

E-mail: ******@*******.***

************@*****.***

2. D. Dhanasekaran, Ph.D.

Assistant professor,

Department of Microbiology,

Bharathidasan University,

Trichirapalli – 632014.

E-Mail: *******@*****.**.**

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