Scientist / Manager Scientist

Xianghua Xu, Ph.D

*** ***** *** *** **, New York, NY 10033

Phone: 347-***-****

Email: ********.**@*****.***

Work authority: US Permanent Resident

PROFESSIONAL EXPERIENCE

Columbia University, 12/2007-4/2011 (3 years 5 months)

Associate Research Scientist

Lab manager: lab maintenance, funding management.

Pharmacological regulation of Excitation-Contraction coupling by small proteins in cultured heart cells.

Weill Cornell Medical College, 5/2005-5/2007 (2 years 1 month)

Postdoctoral Research Associate

Trafficking research of cardiac membrane proteins, which could cause the Long QT syndrome in hearts.

Baylor College of Medicine, 2/2004-4/2005 (1 year 2 months)

Postdoctoral Associate

Gene transcription regulation by NF-κB in brains of epilepsy.

Institute of Materia Medica, Chinese Academy of Medical Sciences, 9/1999-8/2003 (4 years)

Graduate Research Assistant

Gene expression and functional changes of potassium channels in Alzheimer’s disease and cerebral ischemia.

Successfully established cell-based high throughput screening of ion channel regulators.

SPECIALTIES

• Electrophyiology: whole-cell patch clamp from cell lines/ neurons/ cardiac myocytes (7 years experience)

• Cell culture techniques (cell lines or primary dorsal root ganglia or hippocampal neurons /cardiac myocytes cultures) (5 years experience)

• Protein expression and cell-based assays. (6 years experience)

• Immunocytochemistry and laser confocal microscopy images, intracellular Ca2+ images (5 years experience)

• Lentivirus and adenovirus preparation and purification (3 years experience)

• Membrane protein isolation and Western Blotting (3 years experience)

• Gene clone and mutagenesis for constructors (5 years experience)

• RNA/DNA extraction and expression assays; qPCR (10 years experience)

• Mouse/rat tail DNA extraction and genotyping (1 year experience)

• Animal experiments: small rodents handling, animal models, behavior test (5 years experience)

EDUCATION& DEGREES

8/2003 Ph.D. of Pharmacology

Chinese Academy of Medical Sciences (Peking Union Medical College of Tsinghua University), Beijing, China

7/1998 B.S. of Pharmacology

China Pharmaceutical University, Nanjing, China

SELECTED PUBLICATIONS

1. Xu X, Marx SO, Colecraft HM. Molecular mechanisms, and selective pharmacological rescue, of Rem-inhibited CaV1.2 channels in heart. Circulation Research. 2010; 107(5):620-30.

2. Yang T, Xu X, Kernan T, Wu V, Colecraft HM. Rem, a member of the RGK GTPases, inhibits recombinant CaV1.2 channels using multiple mechanisms that require distinct conformations of the GTPase. Journal of Physiology. 2010; 588(Pt 10):1665-81.

3. Xu X, Colecraft HM. Engineering Proteins for Custom Inhibition of CaV Channels. Physiology. 2009; 24(4): 210-8.

4. Xu X, Colecraft HM. Primary culture of adult rat heart myocytes. Journal of Visualized Experiments. 2009 (28). pii: 1308. doi: 10.3791/1308.

5. Xu X, Kanda VA, Choi E, Panaghie G, Roepke TK, Gaeta SA, Christini DJ, Lerner DJ, Abbott GW. MinK-dependent internalization of the IKs potassium channel. Cardiovascular Research. 2009; 82(3): 430-438.

6. Roepke TK, Kontogeorgis A, Ovanez C, Xu X, Young JB, Purtell K, Goldstein PA, Christini DJ, Peters NS, Akar FG, Gutstein DE, Lerner DJ, Abbott GW. Targeted deletion of KCNE2 impairs ventricular repolarization via disruption of I(K,slow1) and I(to,f). FASEB Journal. 2008; 22(10): 3648-3660.

7. Abbott GW, Xu X, Roepke TK. The impact of ancillary subunits on ventricular repolarization. Journal of Electrocardiology. 2007; 40(6 Suppl): S42-46.

8. Lubin FD, Ren Y, Xu X, Anderson AE. Nuclear factor-κB regulates gene transcription and seizure threshhold following convulsant stimulation. Journal of Neurochemistry. 2007; 103(4): 1381-1395.

9. Pan Y, Xu X, Wang X. Messenger RNA and protein expression analysis of voltage-gated potassium channels in the brain of Abeta(25-35)-treated rats. Journal of Neuroscience Research. 2004, 77(1): 94-99.

10. Ren Y, Xu X, Wang X. Altered mRNA expression of ATP-sensitive and inwardly rectifier potassium channel subunits in streptozotocin-induced diabetic rat heart and aorta. Journal of Pharmacological Sciences. 2003, 93(4): 478-483.

11. Pan Y, Xu X, Wang X. Rivastigmine blocks voltage-activated K+ currents in dissociated rat hippocampal neurons. British Journal of Pharmacology. 2003, 140(5): 907-912.

12. Xu X, Pan Y, Wang X. Alterations in the expression of lipid and mechano-gated two-pore domain potassium channel genes in rat brain following chronic cerebral ischemia. Molecular Brain Research. 2003, 120(2): 206-210.

13. Xu X, Pan Y, Wang X. mRNA expression of the lipid and mechano-gated 2P domain K+ channels during rat brain development. Journal of Neurogenetics. 2002, 16(4): 263-269.

14. Xu X, Pan Y, Wang X. mRNA expression alterations of inward rectifier potassium channels in rat brain with cholinergic impairment. Neuroscience Letters. 2002,322(1): 25-28.

15. Ren Y, Xu X, Zhong C, Feng N, Wang X. Hypercholesterolemia alters vascular functions and gene expression of potassium channels in rat aortic smooth muscle cells. Acta Pharmacologica Sinica. 2001, 22(3): 274-278.

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