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Laboratory Technician

San Antonio, TX, 78240
November 15, 2009

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**** ******* ***** * San Antonio, TX 78240 * * 732-***-**** (Cell) *



Seeking a position in the Bio-Tech/Pharmaceutical Industry that will utilize my educational background and experience.




Bachelor of Science; Biotechnology Graduated: January, 2009.


Glassboro, New Jersey Completed: 30 credits; Biological Science GPA 3.3 May, 2005 Dean’s List 2004-2005, National Dean’s List in 2005-2006


Skills/Lab experiences:

 Maintaining laboratory equipments such as incubators, pipette cylinders, aspiration pump, cell counter, and water baths.

 Maintaining mammalian cell lines using general cell culture techniques. Prepare cell culture media.

 Counting cells using Hemocytometer and electronic counter, measuring pH and osmolality.

 Subculture of continuous cell line growing in monolayer.

 Staining of monolayer cell culture with geimsa. Acquired knowledge of Gel Electrophoresis, PCR.

 Aseptic Techniques, pure culture techniques, follow basic experimental protocols.

 Staining and observing microorganisms: smear preparation, simple stain, gram stain, spore stain, acid-fast stain.

 Chromatography: Gel Filtration, Ion Exchange (Cation & Anion), High Pressure Liquid chromatography, Affinity chromatography. Acquired knowledge of SDS-Polyacrylamide gel (PAGE) technique.

 PC proficiency in windows: Word, Excel, PowerPoint, Adobe Acrobat, and Access.



Presentation Topic - Biotech-DNA Fall 07 - William Patterson University, Wayne NJ

 “A Rapid and Highly Specific Technique to Detect Hepatic C Virus using RT-PCR Technique”

Lab Report/ Group Project - Biotech-DNA Fall 07 - William Patterson University, Wayne NJ

 “Isolation of DNA from liver tissue, plasmid, and corn”

 “Aseptic cell-culture Techniques” “Titration”

 “Tobacco Transformation and Regeneration”

 “DNA PCR; Cloning of DNA.”

Lab Report/Group Projects - Biotech-Cell Culture Spring 08 –William Paterson University, Wayne NJ

 “Sunflower Callus Experiment”

 “Carrot Somatic Embryogenesis”

 “Growth Curve for RAW 264.7 cells and 3T3 cells”

 “Cytotoxic analysis of RAW cells using LPS”



Life Cell Corporation/A KCI Company. Branchburg, New Jersey Feb. 2009-Oct. 2009

Duties Include:

 Performs tissue (porcine) processing activities such as de-bristling, assessment, epidermis removal of Life Cell Tissue Matrix (LTM). This process also involves cutting (sizing), and packaging.

 Performs solutions change on tissue (porcine), store tissue in blast chiller, -80 degree freezer and in an incubator.

 Trained in clean room operations: gowning and working in Class 100,000-100 clean room environments.

 Performs the primary and secondary packaging of tissue in the clean room.

 Enters date into Oracle base files and records information on batch records.

 Follow experimental protocols. Monitor internal inventory levels. Clean and maintain lab equipments.

 Working with biohazardous materials, aseptic technique, and sanitation of clean room facilities.

 Operate on autoclave by loading and unloading, biohazard laminar flow hoods, bar code scanning equipment, aspiration systems, weighting scales, computer to enter data and generate reports.

Comfort Keepers Flemington, New Jersey October 2006 – Jan. 2009

Home Instead East Windsor, New Jersey Feb. 2005 – Oct. 2006

Duties Include:

 Maintain a therapeutic environment in the homes of patients.

 Utilize behavior modification techniques in the management of assaultive behavior when taking medication.

 Collaborate with professional staff (Psychiatrist, R.N. or other Mental Health Personnel) in providing therapeutic services.

 Organize and maintain medical records of clients.

 Collect lab specimens; Drive patients and assist them as they prepare for various clinics and diagnostic tests, such as EKG, X-Ray etc. and follow-up treatments.

 Provide companionship and an avenue for verbalizing and ventilating their feelings.

 Provide acute attention to details in troubleshooting and problem solving.

 Assist with activities of daily living, basic nursing care such as bathing, grooming and proper hygiene.

 Acquire knowledge of strong organizational, goal-oriented skills and medical terminology.

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