Maxwell Mianecki
***** ** **** ****, ******, MI *8042
**********@*****.***
Profile
I am a recent graduate of Michigan State University with a B.S. in Biochemistry and Molecular Biology. I am eager to gather new skills and build existing ones. I have a deep and passionate interest in science and am seeking to eventually obtain a PhD in Cellular and Molecular Biology. I am experienced in laboratory work and am readily equipped to learn new techniques. I am hardworking, patient and determined in all work that I do.
Education
2006 - 2010 Michigan State University, B.S. in Biochemistry and Molecular Biology
Subjects/Courses of Note: Biochemistry, Organic Chemistry, Immunology, Entomology, Genetics, Microbiology, Eukaryotic Cell Biology, Biochemistry Laboratory
2002 - 2006 Lutheran High School North
Experience
December 2011 – Current Pet Supplies Plus
Stock Worker
Although this job was intended to be a holdover until a more relevant opportunity became available, I think it is worth mentioning due to the hands-on experience with animals.
• Faced shelves, placed items in appropriate places.
• Customer service
• Managed and took care of animals.
September 2010 – August 2011 Michigan State University Department of Biochemistry
Biochemistry Research Trainee Program
I worked in Professor Min-Hao Kuo’s laboratory, performing experiments relating to the post-translational modification of proteins.
• Performed basic laboratory duties such as autoclaving and mixing up chemicals.
• Worked with E. coli – growth, transformation and induction.
• Conducted DNA gel electrophoresis and SDS-PAGE experiments.
• Performed solubility and purification experiments with proteins harvested from E. coli.
• Worked with enzymatic digestion of proteins and devised lab protocol based on experience.
• Extensive experience with Western Blotting, including rigourous troubleshooting and optimization
• Experience with lab management tasks, such as purchasing items for research.
• Research is still in progress. A paper with my name on it is forthcoming.
June 2010 – September 2010 Hungry Howie’s Pizza
Delivery Driver
Food service work.
• Fast-paced work preparing food.
• Delivered items to customers.
• Cleaning and building upkeep.
February 2009 - May 2010 Michigan State University Department of Toxicology
Research Assistant
I worked in Professor Brad Upham's toxicology laboratory conducting experiments in the field of environmental toxicology.
• Worked with rat liver cells, learning how to culture and care for them.
• Conducted experiments exposing cells to various chemicals, exercised assays to test the exact degree of cell-cell communication through gap junctions.
• Learned good lab notebook-keeping practices.
• Interpreted laboratory data and produced workable numbers. Used Image J to quantify culture image data.
Summer 2008 Macomb YMCA
Child Care Personnel
I worked in child care, supervising children for several hours until their parents arrived to pick them up from day camp.
• Monitored and was responsible for up to 20 children (of ages varying from 5 to 12) at once.
• Interacted with parents and dealt with children, solving any problems that came up.
Summer 2005 - Fall 2007 Macomb YMCA AND Macomb County Recreational Center
Life Guard
Worked in pool supervision and maintenance.
• Learned safety and rescue techniques.
• Worked with pool chemicals and learned pool care and maintenance.
• Interacted with pool guests.
Computer Skills
• Proficient in Microsoft Word, Powerpoint and Excel
• Familiar with Microsoft Access.
• Proficient with the scientific program ImageJ.
• Familiar with other scientific programs such as ClustalX, Visual Molecular Dynamics and PeakSimple.
Laboratory Skills
• Genetic engineering of E. coli.
• Cell culture of rat liver stem cells.
• PCR.
• Use of centrifugation and other lab instruments
• Extraction and purification of proteins from a living sample.
• Lipid chromatography analysis- both gas chromatography and thin-layer chromatography.
• Enzyme assays and analysis.
Interests
Entomology hobbyist; amateur science-fiction novelist; video, table-top and card gamer (organized a table-top gaming and card gaming group.)
Memberships
Member of the Society for In Vitro Biology.
Accomplishments
Papers:
Michigan Society of Toxicology Fall Conference - Endocrine Disruptors, methoxychlor and vinclozolin, affect in vitro biomarkers of tumor promotion and epigenetic toxicity; R. Chopra, E.R. Kumar, M.J. Mianecki, L.D. Goodall, P. Babica, J.S. Park, J.E. Trosko, and B.L. Upham. Department of Pediatrics and Human Development and Food Safety and Toxicology Center, Michigan State University, East Lansing MI. 2009
Certificate of completion for Michigan State University’s Biochemistry Research Trainee Program.
References
Professor Brad Upham, Assisstant Professor in Toxicology, 243 Food Safety and Toxicology Building; East Lansing, MI, 48824 Phone #: 517-***-****, ext 186; E-mail: *****@***.***
Dr. Pavel Babica, Deputy Head of the Centre for Cyanobacteria and Their Toxins; Institute of Botany, Academy of Sciences of the Czech Republic; Lidicka 25/27, Brno, CZ65720, Czech Republic; Phone: +420-***-***-***; Email: ******@***.***
Professor Chris DiFonzo, professor of Entomology at Michigan State University, 43 Natural Science Building East Lansing MI 48824; Phone: 517-***-****; email: *******@***.***
Professor Zachary F. Burton, Professor of Biochemistry, ******@***.***
Professor Min-Hao Kuo, Associate Professor of Biochemistry at Michigan State University, ****@***.***, 401 Biochemistry Building Michigan State University East Lansing, MI 48824-1319; Phone #: 517-***-****