Fermentation

RESUME

E.THAMIZH SELVAN, M.Sc, M.Phil.,

Flat No. 30, II Cross street,

Kamatchi Amman Colony,

Pallikarani,Chennai-600100.

Mobile:098********

Email:*********@*****.**.**

OBJECTIVE

I am aiming for high growth and challenging opportunities to use my talent and motivation, in a favorable environment to build on my current competencies. I wish to build up my career by playing a vital role in the biopharmaceuticals and biotechnology industry.

TOTAL EXPERIENCE

7.0 years of Extensive Experience in the field of Fermentation Technology.

EDUCATIONAL QUALIFICATION

M.Phil-Biotechnolgy : Periyar University, 2006-2007

M.Sc-Applied Microbiology : Adhiparasakthi College of Science, Kalavai.

University of Madras, 2001-2003,

B.Sc-Microbiology : Shanmuga Industries Arts & Science College,

Thiruvannamalai, University of Madras, 1998-2001

WORK EXPERIENCE

Company-3 : Shasun Chemicals and Drugs Ltd,

 Designation : Research Officer,

 Department : Research and Development

 Experience : Production of recombinant Therapeutic Proteins

 Period : January 2008 – to Till date

 Location : Chennai.

PROJECTS WORKED

 Production and Purification of GCSF by Recombinant E.coli fermentation.

 Production and Purification of r- Streptokinase by Recombinant E.coli.

 Production and Purification of Interferon by Recombinant E.coli fermentation.

DUTIES & RESPONSIBILITIES

Process Development and Technology Transfer:

 Fermentation Process:

 Maintenance of Master cell Bank and Preparation of Working cell bank, Media and Parameters optimization by shake flask level and pilot plant scale (20 L) fermentation

 Scale up of process up to 100 L Fermentor.

 Trouble-shooting, optimization of industrial processes and commercialization of proprietary fermentation technologies.

 Hands on Experience in Preparation of SOP’s, BPR, and Documentation as per ISO and GMP standard and Coordinate my team

 Downstream process Skills:

 Cell Harvesting, Diafiltration by TFF System,Cell lysis and IBs washing, Solubilization and Refolding of therapeutic proteins.

 Protein Purification Techniques: Filtration, Centrifugation and Chromatography Techniques (HIC, IEX, IMAC) by using K Prime 40 II, AKTA Prime.

 Experience in Buffer Exchange and Concentration of Protein samples by TFF Membrane system

 PEGylation and Purification of proteins

 Immuno & Molecular Techniques:

 Phenotypic and Genotypic characteristics of Escherichia coli strains.

a) Genotypic: Plasmid isolation, Restriction digestion, Amplification by PCR

b) Phenotypic: Plasmid stability, Cultural characterization and Biochemical test.

 Protein Characterization Techniques:

 SDS-PAGE analysis, staining the proteins resolved by Coomassie and Silver staining method.

Company-2 : Malladi Drugs and Pharmaceutical Ltd,

 Designation : Junior Scientist

 Department : Research and Development

 Experience : Production of Fermentation Products

 Period : March 2004 – to Jan 2008

 Location : Chennai.

PROJECTS WORKED

 Production of L-Phenyl acetyl Carbinol (L-PAC) by Saccharomyces sps by fermentation Method

 Production of L-Phenyl acetyl Carbinol (L-PAC) by Bioconversion Method

 Bioconversion of Pyruvate from Lactate by Acinetobacter sps

 Production of Dihydroxy Acetone by Acitobacter suboxydants

 Production of L-Lactic Acid & D-Lactic Acid by Lactobacillus delbrueckii

DUTIES & RESPONSIBILITIES

Process Development and Optimization of Fermentation Parameters.

Company-1 : Health India Laboratories Ltd,

 Designation : Microbiologist

 Department : Quality Control

 Experience : Microbial Analysis of Food Products.

 Period : Oct 2003 – to Mar 2004

 Location : Chennai.

DUTIES & RESPONSIBILITIES

 Total aerobic microbial count

 Total bacterial count

 Total fungal count

 Identification of specific microorganisms

 Escherichia coli

 Salmonella sps

 Pseudomonas aeruginosa

 Staphylococcus aureous.

 Microbiological analysis of Food products, Microbial assessment of water (MPN Techniques),Test for sterility and LAL (Limulus amebocyte lysate) Test

 SOP Preparation and Documentation as per ISO and GMP

 Knowledge in Good Laboratory Practice and Good Manufacturing Practices.

PERSONAL DETAILS

Name : E.THAMIZHSELVAN

Father’s Name : M.Elumalai

Date of Birth : 02-06-1981

Sex : Male

Marital Status : Married

Nationality / Religion : Indian / Hindu

Address for Communication : Flat No. 30, II Cross Street,

Kamatchi Amman Colony,

Pallikarani, Chennai-600100.

Email : *********@*****.**.**

Languages known : English and Tamil

DECLARATION

I here by declare that all the above information is true to the best of my knowledge and belief.

Yours Faithfully

(E .THAMIZHSELVAN)

REFERENCES:

Dr.K.Prabakaran PhD., R.Jayachandran,

Strides Arcolab Limited, Senior Research Associate –CRAMS,

Vice president, Intas Biopharmaceuticals Pvt Ltd,

Bangalore. Ahmedabad,

Mobile: 098******** Ph: +91-271*-******

Off : 080******** M: +91-972*******.

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