Quality Control Sales
Resume:
Samir Adel Alhasan, Ph.D.
Dearborn, MI 48126
********@*****.***
Summary
Scientific Director with over 15 years of experience in the Biotechnology industry and academy, across different fields in the biomedical sciences. Most recently, worked as Director of Cell and Molecular Services at Asterand, Inc., developing and launching cell and molecular services like primary cell isolation from normal and diseased tissues, three dimensional cell culture model, cell based assays, and gene expression analysis services, and extensive experience in tissue banking and tissue procurement sites worldwide. I have extensive experience talking to scientists, physicians, hospital staff, and sales professionals
• Developed and commercialized three dimensional cell culture models and associated cell based assays
• Developed Glucose Induced Static Insulin secretion (GSIS) assay and used it to test customer compounds on human pancreatic islets.
• Developed and commercialized primary cell isolation from different human tissue types and cell based assays
• Developed Automated RNA isolation using Biomek NX robot
• Lead the development and launching of gene expression analysis services using Illumina’s DASL and whole genome direct hybridization
• Developed and wrote molecular quality control SOPs for Asterand
• Built a strong cell and molecular biology team for Asterand
• Developed different protein isolation, and ELISA assays
• Developed IHC SOPS for Asterand
• Implemented GLP at cell and molecular services at Asterand
Professional Experience
Asterand, Inc 2001 to 2010
Asterand is a biorepository and contract research organization with offices in Detroit, MI, San Francisco CA, and Royston UK. It is a leading provider of human tissue based research solutions.
Senior Scientist Director of Cell and Molecular Services 2006 to 2010
Responsibilities:
• Establish and commercialize three dimensional cell culture model, and used to test customer anticancer small molecules and antibodies, negotiate perform and report work on projects
• Manage cell and molecular services and implement GLP practices
• Lead the GSIS assay development and testing on human pancreatic islets
• Manage RNA, DNA isolations and gene expression studies
• Lead cell culture and primary cell isolation
• Manage tissue microarray (TMA) design and construction
Samir Alhasan, PhD. Page 2
• Provide technical support for marketing, sales and customer services departments
• Manage phase zero projects and develop and commercialize cell based assays
• Monitor and update cell and molecular services SOPs as needed
• Provide scientific support for different departments as needed
Accomplishments:
• Within three months reduced the time between placing an order of customer TMAs and shipping them from 12 weeks to 3 weeks
• Trained all staff on becoming GLP compliant
• Established map processes for primary cell isolation, TMA construction and cell based assays
• Launched 3D cell culture service and generated more than $100,000 in revenues in 2009.
• Lead the launching of gene expression analysis
Senior Scientist Director of Molecular Biology R&D 2003 to 2005
Responsibilities:
• Technical support for different departments
• Develop new products and techniques, Establish primary culture of Chodrocytes and Synovial fibroblasts, and characterize them with immunohistochemistry. Establish primary cell cultures from different normal and tumor tissue types like breast, lung, prostate, head and neck and colon, and perform phase zero clinical trials and cell based assays, on the isolated cells
• Establish and lead GSIS assay to test the effect of anti diabetes drugs on pancreatic, and hepatocytes isolation teams. Negotiated and reported results to customers.
• High throughput antibody screening with TMAs and Immunohistochemistry
• Provide Immunohistochemistry services for in house research and customers
• Automation of RNA isolation from human tissue, and isolation of miRNA
• Establish protein products like protein arrays, ready to use western blot gels and antibody target validation
• Provide training on tissue banking operations for new procurement sites
• Testing mechanism of action of anticancer new compounds for customers using both cell lines and primary cells (phase zero clinical trials)
Achievements:
• Launched primary cell isolation services which generated in $500,000 revenue last year only
• Delivered to the production lab an automated protocol for RNA isolation using Biomek NX robot
• Established GSIS assay that generated $150,000 last year only
• Launched primary cell based assays which generated $600,000 in revenues last year
Samir Alhasan, PhD. Page 3
Senior Scientist/Director of Molecular Biology 2001 to 2003
Responsibilities:
• Supervise all molecular biology activity, design and perform molecular quality control experiments to test the quality of Asterand’s tissue inventory
• Established RNA and DNA banks
• Technical support for sales and other departments and management.
• Work with histology group to characterize tissue samples, utilizing H&E staining in addition to Immunohistochemistry
• Create tissue procurement SOPs, and train donor sites on tissue procurement
• Set, train and audit tissue donor sites to insure compliance with SOPs and ethical regulations, worldwide
Achievements:
• Established SOPS for RNA, DNA and protein isolation from different tissue types and cleared a backlog of 3000 tissue samples that needed quality testing , in my first two months
• Established RNA, DNA and total tissue lysates as product offered by Asterand
• Trained and audited tissue procurement sites within the USA and overseas
Wayne State University/ Karmanos Cancer Institute 1995 to 2001
Senior Research fellow Molecular Pathology
Responsibilities:
• Testing and identifying mechanisms of action for anti cancer agents in cell lines and xenographted tumors in mice utilizing immunohistochemical and western blot techniques. Compounds tested Genistein, and Flavopiradol
• Design and perform experiments in molecular biology and chemoprevention of breast, and head and neck, and prostate cancers utilizing tissue culture, protein purification and detection techniques including immunoaffinity precipitation, IEF and two dimensional SDS_PAGE and western blot analysis molecular biology techniques utilizing DNA affinity precipitation, in addition to southern, northern, and southwestern blots, gene cloning, differential display. PCR, RT-PCR and DNA and RNA isolation
• Study the effect of chemo preventive compounds on signal transduction and cell cycle regulation
• In vivo modeling of tumor, antibody generation and purification, Telomerase assay and micro metastasis of breast and prostate cancer
• Supervise and train postdoctoral fellows and research assistants
Achievements:
• Produced publications on molecular biology and chemoprevention of breast, prostate and head and neck cancer.
• Contributed significantly to grant applications and funding for the lab.
• Trained three postdoctoral fellows and 4 research assistants on cell culture, western blot and molecular biology techniques
Samir Alhasan, PhD. Page 4
University of Iowa, Department of Internal Medicine 1992 to 1994
Postdoctoral Fellow
Responsibilities:
• Design and perform experiments in molecular immunology utilizing RT-PCR, western blot, small animal handling, Radioisotope handling
• Regulation of apoptosis in murine thymocytes utilizing flow cytometry
• Mechanism of signal regulation by Fcγ receptors in B cells
Achievements:
• Contributed to grant writing and publications
• Monoclonal antibody purification
Education
Wayne State University, Detroit, MI
Doctorate of Biology and Immunology, 1992
Wayne State University, Detroit, MI
Master of Science Degree, Major: Biological Sciences
An-Najah National University,
Bachelor of Science Degree, Major: Biological Sciences
Professional Affiliations
• American Association for the Advancement of Science (AAAS), 1987
• American Association for Cancer Research (AACR), 1998
Publications
1. Alhasan S, Richey S, and Desai A. Drug Discovery tutorial: Sourcing Well-Characterized Biospecimens. Xpress Bank family of products has Applicability in Biomarker Research. Genetic Engineering News. December 2008.
2. Marur S, Eliason J, Heilbrun LK, Dickow B, Smith DW, Baranowski K, Alhasan S, Vaishampayan U. Phase II trial of capecitabine and weekly docetaxel in metastatic renal cell carcinoma. Urology. 2008 Oct;72(4):898-902
3. Alhasan S, Eason-Butler J, Walter R, P and N, Smith C, and Eliason J. Changes in the transcriptome of human tumor cells following dissociation and culture. AACR 97th annual meeting, Los Angeles CA, April14-19, 2007
4. Haller A, Kanakapalli D, Walter R, Alhasan, S, Eliason J and Everson R. Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy. BMC Clin. Path. December 2006.
Samir Alhasan, PhD. Page 5
5. Haller A., Walter R., Kanakapalli D., Alhasan S., Everson R., Eliason J. Comparison of Gene Expression Profiles in Cell Line Pairs Derived from Tumor and Adjacent Normal Prostate Tissues. AACR 97th annual meeting, Los Angeles CA, April14-19, 207
6. Cortese J, Everson R G, Gowda A, Alhasan S, Walter R, Sun Y, Wali A, Pas H, Eliason J, Everson RB. Microarray expression- profiling of Gefitinib-sensitizing EGFR mutations in formalin-fixed paraffin embedded adenocarcinoma of the lung. AACR special conference, San Diego , CA , 2005
7. Alhasan SA, Shen X, Ahmadmehrabi M, Reed K, Berry-Green C, Eliason JF. Tissue microarray: a High-throughput approach for analyzing molecular marker expression in archival cancer specimens using immunohistochemistry. (Abstract). Proceedings of the AACR 94th annual meeting 2nd Edition. Washington DC, July 11-14, 2003
8. Chinni SR, Alhasan SA, Multani AS, Pathak S, Sarkar FH. Pleotropic effects of genistein on MCF-7 breast cancer cells. Int. J Mol Med. 12(1): 29-34, 2003.
9. Upadhyay S, Neburi M, Chinni S, Alhasan S, Miller F, Sarkar F. Differential effects of genistein on normal and malignant cells is mediated by p21WAF1. Clin. Cancer Res 7: 1782-1789, 2001.
10. Aranha O, Zhu L, Alhasan S, Wood DP Jr, Kuo TH, Sarkar FH. Role of mitochondria in ciprofloxacin induced apoptosis in bladder cancer cells. J Urol. 167(3): 1288-1294, 2002
11. Glazyrin A, Chinni S, Alhasan S, Al-Katib A, Vaitkevicius V, Sarkar F. Molecular mechanisms of Taxol activation of CD95 mediated apoptosis in pancreatic cancer cells. Int J Oncol. 20(1): 201-205, 2002.
12. Li Y, Bhuyian M, Alhasan S, Senderowicz A, Sarkar F. Induction of apoptosis and inhibition ofc-erbB-2 in breast Cancer cells by Flavopiridol. Clin. Cancer Res. 5: 223-229, 2000.
13. Alhasan SA, Alonso MD, Ensley JF, Sarkar FH. Genistein-induced molecular changes in a squamous cell carcinoma cell line of the head and neck. Intl. J. Oncol. 16:333-338, 2000
14. Alhasan SA, Alonso MD, Ensley JF, Sarkar FH. Genistein induced growth inhibition and apoptosis in squamous cell carcinoma cell line of the head and neck. Nutr & Cancer, 34(1): 12-192, 1999.
15. Ashman RF, Peckham D, Alhasan SA, Stunz L. Membrane unpacking and rapid disposal of apoptotic cells. Immunology Letters, 48: 159-166, 1995.
16. Rosenspire A, McCabe M, Bodepuda S, Alhasan S. Effect of sulphydryl reactive reagents on tyrosine phosphorylation and signal transduction. (Abstract), 9th International Congress of Immunology, 1995.
17. Alhasan S, Santini R, Li Q, Rosenspire A. Membrane immunoglobulin is characterized by distinct structural subpopulations. Molecular Immunology, 30: 593-601, 1994.
18. Kaplan M, Alhasan S, Rosenspire A, and Sundick R. Characterization of stimulatory molecules in avian T cells. Scandinavian Journal of Immunology, 38:1356-1362, 1993.
19. Li Q, Alhasan S, Rosenspire A. Dynamical Aspects of the B Cell Receptor Complex. (Abstract) FASEB. Journal, 9: 261 A, 1993.
20. Alhasan, S. The Structure of the Murine B Cell Receptor Complex. Ph.D. Dissertation. Wayne State University, Detroit, MI, 1992.
Samir Alhasan, PhD. Page 6
21. Alhasan S, Li Q, Montgomery B, Rosenspire A. Analysis of membrane immunoglobulin associated peptides.(Abstract) FASEB Journal, 6:1704, 1992.
22. Chung C, Alhasan S, Rosenspire A. G protein mediated signal transduction: membrane immunoglobulin associated phosphoproteins in Octyl- ß- glucoside lysates of normal B cells. Immunology Letters, 32: 193,1992
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