Analytical Chemist

Eric E. Pullen

Education

Bachelor of Science in Biology and Bachelor of Arts in Chemistry

August 1999 University of North Carolina at Charlotte

GPA: 3.8 (Magna Cum Laude)

Continuing Education:

Introduction to Biochemistry (NC State University)

Modern Liquid Chromatography (GlaxoWellcome)

Practical LC/MS (ASMS)

Micromass training (Waters)

Antek CLND Training (Eli Lilly)

3 day private course on HPLC and SFC (Novasep)

Military Experience

United States Army Veteran

1991 – 1995

Work experience

2010- Present Merck Wilson. NC

Chemist II, Contractor

Currently work at the North American Stability Unit (NASU). Currently test and analyze Late Stage, Release and Final Products to ensure their chemical properties are still viable for human consumption. In a highly regulated GMP / 5S /Six Sigma / Lean laboratory environment, have learned the theory/operation of the following instrumentation; UV-VIS (PE 25), Dissolution Units (VanKel w/autosampler and Distek), Water's Alliance 2690 HPLC and Acquity H-Series UPLC units (Empower software). Other software utilized are LIMS, Trim, and Report Launcher . Knowledge of QA procedures including, but not limited to; Quality Notifications (QN’s), data review and approval (or not), SAP training (ongoing), HEP (Human Error Prevention), sample storage (Temp/RH) and pull dates. Implemented process within the laboratory to improve efficiencies by shortening solution preparation times.

2008 - 2009 Regis Technologies Morton Grove, IL

Separation Scientist

Screened incoming achiral and chiral samples via Thar Discovery Unit and Agilent HPLC 1200 for the best resolution of the desired compound under GMP conditions. If applicable, would perform loading and solubility studies by SFC. If and when the sample was amenable for SFC conditions, purification was performed. Utilized both the Thar SFC 80 and the Thar SFC 350 to achieve excellent separation of the respective enantiomers.

2004- 2007 GlaxoSmithKline RTP, NC

Scientist

Perform method development on both chiral and achiral molecules to achieve maximum resolution

on an analytical SFC instrument. The data obtained is used to purify large quantities of compounds on

a prep SFC unit. Responsible for maintaining an open-access UV analytical system. Developed, implemented and maintained an open-access mass-guided purification system.

2000 – 2003 Eli Lilly RTP, NC

Chemist

Solely responsible for providing purification support to over 40 synthetic chemists using mass-directed prep systems. Also provided guidance to other projects using a variety of techniques such as LC, FIA-MS, LC-MS, IR, and EA. Purified 12,000 and 7,000 samples in 2002 and 2003, respectively.

Software

Hands on use and knowledge of Water’s, Agilent, Berger, Novasep, Gilson, Sciex, and Tecan software.

Presentations • "Glycocalyx Expression by Internalized S. aureus Cells". E.E. Pullen, W.K. Ramp, and M.C. Hudson. American Society for Microbiology, 98th General Meeting, 1998.

• “Tris (pyrazolyl) silane complexes of the group 6 metals". D. Rabinovich and E.E. Pullen. 219th American Chemical Society National Meeting, 2000.

• “Open-Access Prep LC-MS using an Agilent MSD”. Eric E. Pullen and Manon Villeneuve. CoSMoS 2005.

• Dry on the Fly (GSK - SFC 2007 – Pittsburgh, PA)

• A Novel Chiral Stationary Phase (Regis Technologies)

Publications

• "Methyltris (pyrazolyl) silanes; New Tripodal Nitrogen-donor-Ligands". Eric E. Pullen, Arnold L. Rheingold, and Daniel Rabinovich. Inorganic Chemistry Communications 2 (1999) 194-196.

• "One-Dimensional Copper (I) Coordination Polymers based on a Tridentate Trioether ligand". Hing W. Yim, Lihn M. Tran, Eric E. Pullen, Louise M. Liable-Sands, Thomas E. Concolino, Arnold L. Rheingold, and Daniel Rabinovich. Inorganic Chemistry, Vol. 38, No 26, 1999, 6234-6239.

• "Syntheses and Structures of Methyltris (pyrazolyl) silane Complexes of the Group 6 Metals". Eric E. Pullen, Daniel Rabinovich, Christopher D. Incarvito, Thomas E. Concolino, and Arnold L. Rheingold. Inorganic Chemistry, Vol. 39, 2000, 1561-1567.

• “Development of an automated in-line microfiltration system coupled to an HPLC for the determination of solubility “. David H. Igo, Timothy D. Brennan and Eric E. Pullen. Journal of Pharmaceutical and Biomedical Analysis, Vol. 26, No 3, 2001, 495-500.

• "Structural Characterization of 3-tert-butylpyrazole and its nickel (II) chloride complex”. Pullen, E. E, Schimek, G, Pennington, W, Maffett, S, Rabinovich, D. Manuscript in preparation.

• “Non-Polar Reverse Phase Columns under SFC Conditions”. Eric E. Pullen, Keith H. Hornberger, Manon Villeneuve and Luke Miller. LC-GC (on-line---------).

Awards received • GlaxoSmithKline – Silver Award, Bronze Award.

• Eli Lilly - 2001 Q1 Change the World Award

• Phi Kappa Phi Scholar of the Year; College of Arts and Sciences; Math and Science Division, 1996-1997

• The Army Achievement Medal (2)

• Carolina Chemistry Club Scholarship Award, 1998

References Available upon request

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