Customer Service Sales

Vivek K Madem

**** * **** ****** **** Newport Beach, CA 92663 Cell no: 806-***-**** *****.******@*****.***

OBJECTIVE

Entry level Research Associate in Protein purification.

EDUCATION

MS Biotechnology, May 2011 BS in Biotechnology, May 2008

Texas Tech University, Lubbock, Texas Vellore Institute of Technology, India

Overall GPA: 3.57/4 Overall GPA: 3.12/4

TECHNICAL SKILLS & INSTRUMENTS

Laboratory: DNA and RNA extraction • Plasmid isolation • Applied PCR techniques • Cloning and Sub- cloning • transformation of cells • plating • agarose gel electrophoresis • cell lysis techniques • Purification techniques for His- tagged and non His- tagged proteins • Ion- Exchange Chromatography • Nickel- Affinity Chromatography (manual and with Biocad) • Size- Exclusion Chromatography • Bradford assay • SDS- PAGE • Western blotting • Gateway cloning • Site- directed Mutagenesis.

Computer skills & Lab instruments: Microsoft Office • Word • Excel • PowerPoint • C, C++ & Java • Photoshop • Vector NTI • PyMOL • Applied Biosystems BioCad Sprint • Nanodrop ND1000.

EXPERIENCE

Graduate Research Assistant, Department of Chemistry and Biochemistry

Texas Tech University, Lubbock, Texas June 2010- Present

Expressed, purified and characterized enzymes such as Spinach Nitrite Reductase, Synechocystis Glutamate Synthase, Identified specific protein domains which are involved in the interaction between Ferredoxin and enzymes, Created mutations by Site- directed Mutagenesis at different domains of the protein, Expressed and purified the His- tagged recombinant protein by Nickel- Affinity Chromatography and Ferredoxin Affnity column, Characterized the enzymes from SDS- PAGE, Western Blotting, UV- Visible absorption spectra and activity assay, Served as reviewer during the “TTU Undergraduate Research conference”, trained and motivated new students on various purification techniques.

Course Projects, Center for Biotechnology and Genomics, Texas

X- Ray Crystallography: Spring 2010

Introduced mutations in Glutaredoxin protein, expressed and purified the His- tagged recombinant protein using Nickel- Affinity chromatography. Grew crystals using Hanging drop method at various different buffer conditions and is analyzed by X- ray diffraction.

Methods in Biotechnology: Fall 2009

Cloned, over expressed, purified and characterized recombinant 3α-Hydroxy steroid dehydrogenase.

Related course work: Molecular cloning • Advanced Plant biotechnology • Biomedical Informatics • Immunology.

Customer Service Executive, HSBC Global Resourcing, India Spring 2009

Assisted customers with credit card issues, Worked on a team to develop new strategies to make sales and serve the customers in a much better way.

Intern, AG Biotech Laboratories India limited, Hyderabad, India December 2007- December 2008

Participated in research project for identification of Secondary Metabolites (Terpenoids) from Stevia Rebaudiana by In-vitro and Thin layer Chromatography Techniques.

PUBLICATIONS & ACCOMPLISHMENTS

o “Redox properties and Protein structure of Arsenate Reductase and a Glutaredoxin from Synechocystis sp. PCC 6803”, Submitted to “Biochemistry”.

o TTU Instate Scholarship in 2009 • Poster presentation on “Nanotechnology” • Awarded “Best Employee of the month” for making the best sales.

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