Project Objective

PRAVEEN NATT

**** ***** **** *****, ***: *** 312-***-****

CHICAGO IL 60616 *****@***.***

*****@***.***

OBJECTIVE:

To apply scientific knowledge as a laboratory scientist in research and development in the field of Cell and Molecular biology and to work for the betterment of society.

EDUCATION:

ILLINOIS INSTITUTE OF TECHNOLOGY, CHICAGO, IL

MS. BIOLOGY (CELL AND MOLECULAR BIOLOGY) (2010-2012)

GPA-3.40

ANNA UNIVERSITY, CHENNAI, INDIA

B-TECH BIOTECHNOLOGY

FIRSTCLASS WITH DISTINCTION (2006-2010)

WORK EXPERIENCE:

INTERNSHIP: Summer Internship at Dow Agrosciences, Indianapolis, IN through Kelly Services.

May-Aug 2012 (3 months)

TITLE: Isolation and characterization of UK-2A resistant mutants in Saccharomyces cerevisiae and Magnaporthe grisea.

Isolation of resistant mutants. Screening for cross-sensitivity with other commercially available products.

DNA extraction and amplification of cyt b gene. Sequencing and aligning with the wild type for mutant selection.

INTERNSHIP: Summer Internship at Dow Agrosciences, Indianapolis, IN through Kelly Services.

May-Aug 2011 (3 months)

TITLE: Real-Time PCR as an alternative to screen compounds with fungicide activity using Septoria and Wheat system.

• DNA extraction using Biosprint 96 and DNeasy kit. Plant genomic DNA extraction using CTAB.

• DNA quantification using Picogreen assay.

• Real-Time PCR to detect and quantify the Fungal DNA in Wheat plants inoculated with the fungal pathogen at different time intervals and treated with different concentrations of fungicides.

• Determination of effect of fungicides on the amount of fungal DNA.

PROJECT:

TITLE: ISOLATION AND CHARACTERIZATION OF IFN-ϒ GENE IN CHICKEN CELLS

CENTRE: MADRAS VETERINARY COLLEGE, CHENNAI, INDIA

Jan-June 2010 (6 months)

OBJECTIVE: To compare expression of IFN-ϒ in the PBMCs and spleen cells.

Spleen cells were cultured and RNA was isolated. cDNA was then synthesized and reverse transcriptase PCR was performed. The product was eluted and sequenced. The conclusion was the comparison of expression of IFN-ϒ in the PBMCs and spleen cells.

TRAINING:

SURYA HOSPITAL May-Aug 2007 (3 months)

Blood grouping and transfusion

SREEDHAR BHAT’S LABORATORY May-Oct 2008 (6 months)

Recombinant DNA Technology and Immunological Techniques

LABORATORY EXPERIENCE:

CELL AND MOLECULAR:

DNA and RNA isolation, quantitative and qualitative analysis of DNA, competent cell preparation and screening techniques, restriction digestion, RT-PCR, cloning and transformation, MTS assay, experienced in mammalian and fungal culture,transfection.

MICROBIOLOGY:

Media preparation, sterilization, smear preparation, simple staining, streak plate technique, Gram staining, microscopy.

IMMUNOLOGY:

ELISA, Immunostaining, Western Blot, SDS-PAGE, Agglutination-Precipitation, Flow-cytometry.

BIOCHEMISTRY:

Protein expression and crystallization,SDS-PAGE, western blot, chromatography (column and ion exchange).

ACADEMIC ACHIEVEMENTS:

1. Won 1st prize for presentation of a Poster at SRM University on “The Role of Epigenetics in Cancer”

2. Won 1st prize for presentation of a Poster at Paventhar Bharatidasan College of Institute of Technology on “Stem cells in treatment of cancer”

3. Won 2nd prize for paper presentation on” Endophytic changes on Mangrove “at Government College of technology

4. Presented a paper on “Epigenetics and complex diseases” at Konneru Lakshmiah College of engineering

5. Presented a paper on research project “Antimicrobial activity in spinach leaves” at students- Chemical Conference (S-CHEMCON)

Praveen Natt

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