Medical Part Time

SAMITHA VENU

***, ****** ******, *** # **,

Weymouth, MA – 02191

Email: ************@*****.***

Phone: 617-***-****

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EDUCATION:

M.B.B.S (Bachelor of Medicine and Bachelor of surgery)

(2003 - 2008), Govt. Stanley Medical College, Chennai. India

Residency in Medicine and Surgery (2008 – 2009), Govt. Stanley Medical Hospital, India

Certificate in Clinical Data Management (August 2011)

Certificate in Clinical Research (September 2011)

Certificate in GCP (February 2012)

LABORATORY SKILLS:

PCR, cDNA synthesis, Reverse Transcription, DNA and RNA isolation, plasmid extraction, gene cloning, vector design and construction, gene knock-out and knock in studies, southern and northern blot, protein estimation (spectophotometry), affinity column, Ion exchange and gel filtration (chromatography), recombinant protein expression in E. coli, protein purification and Immunization, competent cell preparation, Mammalian and RBC cell culture techniques and transfection techniques, Lentivirus techniques, siRNA techniques, generation, propagation and isolation of stable integrant clones, propagation and isolation of viruses and viral titre (TCID50), adenovirus techniques, lentivirus Techniques, trained to work with mice, rabbits and frogs, Able to perform Intra peritoneal, subcutaneous injections, light microscopy, fluorescent microscopy, ELISA, immunoprecipitation, SDS-PAGE, western blotting, Giemsa and HE staining.

RESEARCH EXPERIENCE:

Research associate (July 2011 – Present): Boston University Medical Center, Boston, MA.

Working on project entitled “identify the roles of Topoisomerase in cancer cell lines (colon cancer, breast cancer, lung cancer, HEK293, stable cell lines and human tissues).

Performing all molecular biology techniques and maintain the mammalian cell culture.

Delivered periodic presentations of results and works in progress, to research staff.

Preparation and maintenance of lab stocks and records.

Research associate (January 2010 – June 2011): Drexel University College of Medicine. Philadelphia, PA.

Worked on two projects independently;

1. Oxidative stress-mediated apoptosis of rat hepatocytes exposed to acute ethanol treatment in the presence of Hepatitis B virus X protein.

2. Hepatitis B Virus X protein Regulation of Autophagic Responses – Calcium, NFKB pathway.

I handled hepatoblastoma, hepatocellular carcinoma cell lines, HEK293 cell lines and human tissues.

Gained experience in mammalian cell culture techniques and transfection, propagation and isolation of viruses and viral titre (TCID50), Adenoviral transfection, Western blotting, siRNA techniques.

Performed liver dissection and collected hepatocytes from rats.

Summer Intern (March 2008 - September 2008): Drexel University College of Medicine, Philadelphia, PA.

Assisted a graduate student on 2 different projects;

1. Role of Malate quinone Oxidoreducatse and NADH dehydrogenase in blood stage Plasmodium falciparum

2. Characterization of Aminomethyltransferase like protein in blood stage P. falciparum

Performed gene cloning, vector design and construction, gene knock-out and knock in studies, RBC cell culture techniques and transfection.

PROFESSIONAL EXPERIENCE:

Residency in Govt. Stanley medical college (2008 - 2009)

R.K hospital, Chennai, India (January 2009 – March 2009) (Part time duty doctor)

Billroth hospital, Chennai, India (April 2009 - August 2009) (Part time duty doctor)

ANALYTICAL SKILLS:

Writing and editing laboratory method and manuals and safety procedures

Statistically analyzing, graphing and summarizing data

Maintaining excellent written and verbal communication skills

Engaging creative problem solving

COMPUTER SKILLS:

Imaging and scanning by using Photoshop

Microsoft Word, Excel and Power Point

BLAST, Vector NTI

Ink scape for drawing scalar maps

ACADEMIC ACHIEVEMENTS, AWARDS AND SCHOLARSHIPS:

Second prize in inter-schools essay writing competition

Secured School first rank in 10th Standard and Higher Secondary

Secured District first Mark in Higher secondary

Recipient of Chief Minister’s Merit scholarship throughout M.B.B.S. degree programme

Got 42nd rank in Tamilnadu Entrance Exam conducted by Anna University, 2003

Awarded Residency fellowship during M.B.B.S degree programme (2008 – 2009)

PUBLICATIONS:

1. Ankur Shah, Samitha Venu, Ajit Bharti. Mechanism of BRCA1 mediated ubiquitination and degradation of topoi by the ubiquitin proteosomal pathway in TNBC. (Ready for submission)

2. Suresh, M.G., Joanne M. Morrisey, Samitha Venu, Praveen, B.N, Michael W. Mather and Akhil B. Vaidya. Molecular and genetic characterization of Malate: Quinone oxidoreductase enzyme in Plasmodium falciparum. (Ready for submission)

3. Suresh, M.G., Joanne M. Morrisey, Samitha Venu, Michael W. Mather and Akhil B. Vaidya. Role of aminomethyl transferase enzyme in malaria parasites (In preparation)

REFERENCES:

Will be given on request.

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