oligo-chemist

SUMMARY: Highly motivated, hard working organic and analytical chemist with over fifteen years of experience in solution and solid phase chemistry. Proven track record in designing multisteps synthesis, developing new synthetic methodologies, lead compound isolation technologies. Unique blend of strong analytical and spectroscopic techniques for purification and structural elucidation of compounds. Excellent problem solve skill.

PROFESSIONAL EXPERIENCE:

07/08-04/09 Staff Scientist

Osmetech Molecular Diagnostics, Pasadena, CA

• Synthesis, purification and analysis of muti-ferrocene labeled oligonucleotide under GMP guideline.

• Developed new HPLC test analysis method for diagnostic product.

• Validation of HPLC test method according USP and ICH regulatory guideline.

• Developed purification process methods for IVD oligonucleotide.

• Transfer synthetic and analytical method to manufacturing.

6/98-7/08 Senior Scientist, group leader,

Biosynthesis Incorporated, Lewisville, Texas.

• Synthesized Oligonucleotide (DNA, LNA, RNA PNA) on automated instrumentation.

• Purification and analysis of Oligonucleotide and peptide by ion-exchange, reverse phase column.

• Designed and synthesized special monomer for oligomer synthesis.

• Designed and synthesized DNA-PNA, PNA-RNA, LNA-PNA Chim.

• Analysis and characterize molecular by LC-MS, and NMR.

• Developed new method of conjugating RNA to scaffold such as Dendrimer, Polyvinyl alcohol, PEG reagents

• Conjugate Cell-penetrating peptide to RNA, DNA, LNA, PNA.

• Synthesis of modified oligonucleotide such as 5’ or 3’ thiol modified oligo, 5’ or 3’ amino modified oligo, labeling oligo using many different dye such as cy3, cy5, FTTC, Ferrocene, Dig and more.

• Communicated with customer their research needs and help to design their oligo.

• Supervise technician how to synthesis and purify oligos and its conjugates.

• Training technician maintains synthesizer and HPLC instrument

• Designed and synthesized chemically modified peptides. (Solid phase chemistry)

Lipid peptides, cyclic peptide (single or double disulphide bridge, head and tail cyclic), Mono or poly Phosphorylated peptide, Mono or poly Sulfated peptides. Rhodamine/Fluorescein/Biotin labeled peptides and more.

Customer organic synthesis projects:

• Designed and synthesis of acylated saponin derivatives having adjuvant activity.

• Designed and synthesized amphipathic compounds having adjuvant activity.

• Interacts with customer, designed and synthesized many intermediate compounds for pharmaceutical company.

11/91-6/98: Research Scientist, Department of Biochemistry, UT Southwestern Medical Center, and Dallas, Texas. (Under supervision of Dr. J. R. Falck.)

• Designed and synthesized many anti-cancer and anti-diabetic compounds.

• Developed new analytical method to characterize inositol compound.

• Carbohydrate based asymmetric synthesis of inositol polyphosphates.

• Solved several problems encountered during the synthesis of many molecules.

• Handled air sensitive Boron and Tin compounds.

• Asymmetric synthesis of secondary alcohols and secondary amines.

• Collaborated with many pharmacalogists, and biologists in diabetic research.

• Wrote research reports and papers.

• Trained technicians in drug discovery projects.

INSTRUMENTATION AND TECHNIQUES:

• Expedite 8909 DNA synthesizer, ABI 392/394 DNA synthesizer, Mermade DNA synthesizer (trouble shooting skill), ABI 431433 peptide synthesizer, Agilent LC-MS, MALDI Mass spectrum, UV, IR, NMR (1D and 2D NMR techniques), HPLCs (trouble shooting skill), Perkin Elmer Polarimeter, Chromatographer, SPECTRAmax Plus384.

• Computer skill: MS Word, Chem. Drawing, Scifind.

EDUCATION:

PH.D. (Organic Chemistry), Shanghai Institute of Materia Medica, Chinese

Academy of Sciences, P. R. China.

B.Sc. (Chemistry), Department of Chemistry Nanjing University, P. R. China.

References are available upon request.

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