Assistant Maintenance

Rachel Shira Donsky

** ********* ** *** * Allston, MA 02134

Home: 617-***-**** Cell: 617-***-**** Email: **********@*****.***

OBJECTIVE

Seeking a Molecular Biologist Research Assistant position in a Pharmaceutical Company focused on disease mechanisms and pre-clinical drug development.

EDUCATION

Bachelor of Science, Diagnostic Genetic Sciences

University of Connecticut, Storrs, CT

Graduated Magna Cum Laude

2002-2006

WORK EXPERIENCE

2007-present: Technical Research Assistant in the laboratory of Jonathan A. Fletcher, MD at Brigham and Women’s Hospital

Maintenance of Cell Cultures and Establishment of Unique Sub-lines

Ø Excellent sterile tissue culture technique

Ø Maintenance of various GIST, Mesothelioma, Liposarcoma, and other cell cultures

Ø Development of several unique inhibitor resistant sub-lines by treatment with clinically relevant drugs

Ø Cell freezing and thawing

Ø Sterility testing

Molecular Analysis

Ø Nucleic acid isolation

Ø PCR, RT-PCR

Ø Genomic and allelic sequencing

Ø Western blotting

Ø Plasmid cloning

Ø Transfection

Ø Cell viability assays

Ø Lentiviral mediated RNAi

General Lab Maintenance

Ø Preparation of media and solutions

Ø Inventory and ordering of supplies

Ø Lab organization and upkeep

Ø Troubleshooting of problems with reagents and equipment

ACCOMPLISHMENTS

Poster Abstracts:

Jonathan A. Fletcher1, Maria Debiec-Rychter2, Stephen Swank1, Jennifer Morgan1,

Bernadette Liegl1, Tanya A. Rege1, Michael C. Heinrich3, Christopher C. Corless3,

Rachel Donsky1, Christopher D.M. Fletcher1, Chandrajit P. Raut1,Wenbin Ou1, Kevin Foley4, YuexiangWang1, George D. Demetri5. “Hsp90 inhibitor STA-9090 potently suppresses heterogeneous KIT kinase-domain mutations responsible for gastrointestinal stromal tumor progression during imatinib therapy” presented by Jonathan Fletcher at AACR-NCI-EORTC November/2009, Boston, MA.

Publications:

Yuexiang Wang1, Tanya Rege1, Christopher L. Corless2, Cher-wei Liang1, Stephen Swank1, Rachel Donsky1, Christopher D.M. Fletcher1, Kevin P. Foley3, Daniel L. Flynn4, George D. Demetri5, Chandrajit P. Raut6, Michael C. Heinrich7,8, Jonathan A. Fletcher1 “Molecular heterogeneity of kinase inhibitor resistance mechanisms in gastrointestinal stromal tumors revealed by 454 pyrosequencing” Manuscript in preparation

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