Cv_mass spectrometry/hplc/lcmsms/research/engineer

DEEPTI SHARMA

*** ****** ****, ********* # ** - *D, Ithaca, New York 14850

e-mail: ******.******@*******.***, phone: 1. 817. 504 8161

PROFESSIONAL OBJECTIVE

Contribute effectively through skills in research and development, technical and application support, clinical studies. Additionally, further augment my skills with appropriate yet versatile work experience and technologies

RESEARCH SKILLS

Expertise in bio-analytical instruments: Triversa Nanomate, RePlay; mass spectrometers: 4000 QTrap, LCQ Deca, Water’s QTof and mass spectrometry techniques: MALDI-TOF, tandem MS, LC/MS and LC/MS/MS

EDUCATION

Masters of Science in Bio-Medical Engineering, University of Texas, Arlington, USA.

Jan 2004 – Dec 2005

Bachelors of Engineering in Instrumentation and Control, South Gujarat University, India.

1998 – 2002

PUBLICATIONS

Pegah R. Jalili, Deepti Sharma, and Haydn L. Ball (2007). Enhancement of Ionization Efficiency and Selective Enrichment of Phosphorylated Peptides using Polyhistidine-tags. Journal of American Society of Mass Spectrometry, Volume 18, Issue 6, June 2007, Pages 1007-1017

Baker, J., Burnham, G., Tesoro, J., Sharma, D., & Reyna, V. (2005). Physician medical decision making and clinical guidelines. American Psychological Society

Sharma, D., (2005), Academic paper review, Andreas Bartels and Semir Zeki (2004). Brain dynamics during natural viewing condition: a new guide for mapping connectivity in Vivo. University of Texas, Southwestern, Dallas

PRESENTATIONS

Invited lecture, Express tips for simultaneous bioanalytical determination of Tacrolimus and Rapamycin within one minute run time, 3rd Scientific symposium, Advion, Ithaca, USA, 2009

Seminar presentations, Various topics of biosensors, modern imaging systems, frequency domain near infra red imaging, University of Texas, Arlington, USA, 2006

National level award winning project presentation, Maglev trains, India, 2001

WORK EXPERIENCE

Product and Applications Support Specialist, Advion BioSystems Inc., June 2008 – Present

• Developed analytical methods for quantitation of Immunosuppressant drugs and other compounds relevant to pharmaceutical and life science industry (ABI 4000 QTRAP; Water’s Nano Acuity UPLC)

• Performed research and development of new products in mass spectrometry (Thermo LCQ Deca; Eksigent HPLC)

• Helped in application development in proteomics

• Contributed in technical management of analytical instrumentation and applications support

• Interviewed candidates for field service positions in customer support and service department

• Worked in tandem with engineering, manufacturing and finance staff to support customer issues

• Gained administrative experience by coordinating and scheduling installations and preventative maintenance of instruments

Research Associate, Internal Medicine, UT Southwestern Medical Center, Sep 2006-June 2008

• Executed project on “Quantification of Actin Isoforms” using Protein AQUA method blended with MALDI-TOF, LC/MS, LC/MS/MS and MRM

• Identified novel phosphoproteins in activated hepatic stellate cells using Titanium dioxide phospho-enrichment technique and tandem MS

• Worked with HPLC (high pressure liquid chromatography)

• Gained experience in solid phase extraction (SPE), protein purification from tissue extracts and cell lysates.

• Acquired proficiency in various wet chemistry and biology lab skills

Research Assistant, PCTC, UT Southwestern Medical Center, Texas, May 2005 - August 2006

• Optimized identification of phosphorylation sites using Mass Spectrometric Technique

• Enhanced ionization efficiency and did selective enrichment of phosphorylated peptides

• Honed expertise in Matrix Assisted Laser Desorption Ionization (MALDI) and

Mass Spectrometry Time of Flight (MSTOF) technique

• Performed protein sequencing

• Worked with LC-MS, LC-MS/MS

• Acquired experience in Immobilized Metal Affinity Chromatography (IMAC)

• Obtained Proficiency with various biochemistry techniques like SDS Page, in-gel digestion

Instrumentation Engineer, Waaree Instruments Ltd, India, July 2002 - December 2003

• Designed the flow loop for the automation project at Indian Petrochemicals and Corporation Ltd., India

• Executed installation of pressure and temperature related instruments; calibrated Amtek Zofra calibrators

• Visited industries to supervise for instrument installation and preventative maintenance

• Accomplished sales target for the 3rd quarter - June 2003 - September 2003

Design Engineer, Intern, Indian Petrochemicals Corp. Ltd, Vadodara, India, July 2002-Dec 2002

• Designed pressure, temperature and flow loops using basic instrumentation

• Worked with Distributed Control System (DCS)

OTHER PROJECT DETAILS

Interventions for Risk Reduction and Avoidance in Youth, Reyna Lab, UTA, Project funded by the National Institute of Health, January 2005 - May 2005

Developed protocol utilizing SPSS to examine adolescent judgment and decision making pertaining to behaviors which place them at risk for STD/HIV

Assessed theory based interventions which are designed to reduce risky behaviors

Postmortem volumetric MRI, Radiology Department, UT Southwestern, May 2005 – Dec 2005

Towards post mortem examination of fresh whole brain in AD patients

Acquired experience in handling fixed-human brain

Designing and implementing a set up to scan fresh brain in its in situ conformation in Siemens’s 3.0 T

Deploying an array of tools of image processing like segmentation, normalization, histogram

Near Infra Red Imaging, UTA, 2004

Towards detection of breast tumor using Frequency Domain Photon Migration System

Developed a protocol to calculate HbO2 and Hb total and the results obtained were compared with ISS oximeter

Presented the fundamentals of NIR spectroscopy imaging

PC Controlled Robotic Arm, SCET, 2001 - 2002

To understand principles and functional aspects of robotic arm

Designed and fabricated hardware including integrated circuits on a PC board

Created a program in “C” language to provide motion of 3-degree of freedom

COMPUTER SKILLS

Mass spectrometry software: Analyst version 1.4.2, XCalibur1.3, MassLynx4.0

Programming Experience: C and MATLAB; Proficient in EXPASY, PROWL, MASCOT, SPSS, Microsoft Excel, Microsoft Access,

REFERENCES

Dr. Jack Henion, Chairman and Chief Scientific Officer (CSO), Advion Biosciences

e-mail: *******@******.***; phone: 607-***-****

Dr. Daniel Eikel, Applications Scientist, Advion Biosystems

e-mail: ************@***.**; phone: 607-***-****

Dr. Songling Liu, M.D., Assistant Professor, UT Southwestern Medical Center

e-mail: ********.***@**************.***; phone: 972-***-**** (Cell)

Dr. Simon Prosser, Senior Director, Customer Service and Support, Advion Biosystems

e-mail: ********@******.***; phone: 607-***-****

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