research fellow

RESUME

For Dinggeng (Dean) Chai, PhD

CONTACT

Phone: 856-***-****

Email: ********@*****.***

ACADEMIC CREDNTIALS

Ph.D. from Department of Biological Sciences, University of Calgary 2009

Master of Science from College of Life Sciences, Nankai University 2004

Bachelor of Science from College of Life Sciences, Nankai University 2001

OTHER CREDENTIALS

Team leadership certificate, University of Calgary

Radiation material handling and safety training, University of Calgary

INDUSTRIAL EXPERIENCE AND PROFESSIONAL PRACTICE

Three years of collaborative drug discovery research on osteoporosis (for P&G Pharmaceuticals Co. Ltd. Cincinnati, OH). 2001— 2004

Consultant for Aoteqimeng Medicine Co., Ltd. 2006 to present

Part-time instructor, Mount Royal University January-April, 2009

Postdoctoral research fellow, University of Calgary June 2009 to present

SCIENTIFIC RESEARCH EXPERIENCE

PhD project 2004—2009

Characterization of three-dimensional structure of Lactococcus lactis group II intron.

M.Sc. Project 2001—2004

1. Splicing pattern and localization of a lymphoblastic leukemia gene Lmo2

2. Collaborative drug discovery research on osteoporosis with Proctor & Gamble Pharmaceutical Co. Ltd.

PUBLICATIONS IN PEER-REVIEWED JOURNALS

* Dinggeng Chai. RNA structure and modelling: progress and techniques. Progress in Nucleic Acid Research and Molecular Biology (2008) Vol. 82.

* Lixin Dai, Dinggeng Chai (co-first author), Shan-Qing Gu, Jesse Gabel, Sergei Y. Noskov, Forrest J. H. Blocker, Alan M. Lambowitz, and Steven

Zimmerly. A Three-Dimensional Model of a Group II Intron RNA and Its Interaction with the Intron-Encoded Reverse Transcriptase. Molecular Cell

(2008), 30(4):472-85.

* Dawn M. Simon, Nicholas A. C. Clarke, Bonnie A. McNeil, Ian Johnson, Davin Pantuso, Lixin Dai, Dinggeng Chai and Steven Zimmerly. Group II

introns in Eubacteria and Archaea: ORF-less introns and new varieties. RNA (2008), 14:1704-1713.

* Yang S, Zhao L , Yang J , Chai Dinggeng, Zhang M , Zhang J , Ji X , Zhu T. DeltaEF1 represses BMP-2-induced differentiation of C2C12 myoblasts

into the osteoblast lineage. J Biomed Sci. (2007) Sep; 14(5):663-79.

* Huang Yanping, Kong Deming, Chai Dinggeng, Shen Hanxi, Mi Huaifeng. Gel electrophoresis studying hairpin structure primer on gene detection of

predictive marker for malignant tumor telomerase activity. Modern Instrument (2004), Vol. 4.

CONFERENCE PRESENTATIONS

* Three-Dimensional Modeling of a Group II Intron RNA. RIBOWEST ANNUAL CONFERENCE (Lethbridge, Alberta) –Oral Presentation, June 15th - 18th

2008

* RNA Structure Modeling. ANNUAL BIOLOGY STUDENT CONFERENCE (University of Calgary, Calgary, Alberta) –Poster Presentation, April 17, 2008

* A Three-Dimensional Model of a Group II Intron RNA and Its Interaction with the Intron-Encoded Reverse Transcriptase. GORDON CONFERENCE in

NUCLEIC ACIDS (Newport, Rhode Island) –Oral Presentation, June 3-8, 2007

EXPERTISE

Biochemistry, molecular biology and cellular biology area:

* Mammalian cell culture, transfection, luciferase assay and flow cytometry

* Recombinant and fusion protein construction and expression

* In vivo model, antibody engineering, drug development

* Immunoprecipitation, microarray, northern, southern and western blot

* Clone construction and screening

* Primer extension and manual sequencing

* PCR, RT-PCR, and real-time quantitative PCR

* 5’-RACE, 3’-RACE

* RNA interference and cell signalling pathway profiling techniques

* RNA splicing and photo-crosslinking

Bioinformatics area:

* 3D model construction, optimization and validation

* RNA 2D structure alignment and construction

* 3D and 2D structure presentation

* Experience in using high performance computing grid (distributed computing)

* Molecular Dynamics and Molecular Mechanics (MD/MM)

* Genomic database mining

* Extensive use of bioinformatics tools and knowledge in biostatistics

Computer skills:

* Extensive use of UNIX and LINUX

* Database, Microsoft Office Suite, Google Apps

* Webpage design (Adobe Dreamweaver and Microsoft FrontPage)

* Excellent Hardware and network knowledge, IT maintenance expertise

* Medium level compiling languages (Perl, JAVA, C, Pascal and Basic)

TEACHING AND MENTORING RECORD

* Part-time instructor, Mount Royal College January-April, 2009

(Introduction to Biochemical Molecular Biology, lab sessions BCEM3393-501, BCEM3393-503)

* Teaching assistant at University of Calgary 2006 – 2008

(Molecular Genetics CMMB411)

* Teaching assistant at Medical School, Nankai University 2001 – 2003

(Medical Microbiology, Anatomy, Physiology)

* Mentor for three students, University of Calgary 2007 – 2008

(Bonnie McNeil for RNA splicing; Li Wu for FRET; and Matt Deyell for RNA co-evolution analysis)

* Mentor for two students, Nankai University 2001 – 2003

(Huanshu Tian for setting up expression system, Wei Yuan for RNA protection assay)

HONOURS (AWARDS, FELLOWSHIPS)

* AHFMR teaching postdoctoral fellowship 2009

* Graduate Research Scholarship from University of Calgary 2006-2009

* Chair’s Fund of GORDON CONFERENCE in NUCLEIC ACIDS 2007

* Fee scholarships from University of Calgary 2005, 2007

* Scholarship for Academic Excellence from Nankai University 1997, 1998, 2002

* Excellent Student Leader Awards from Nankai University 1999, 2000, 2001

* Outstanding University Student Award from Board of Education 1998

MEMBERSHIP AND AFFILIATIONS

* Emergency Response Warden, University of Calgary 2007-2008

* Executive board member for CCSSA 2004-2007

* Member of Environment Protection Union of Nankai University 1999-2000

* Chairman of Student Union in College of Life Sciences 1997-2000

* Member of the Young Volunteer Organization in Tianjin 1997-1998

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