Project Quality Control

AKANKSHA PALIAL

**************@**********.*** | 980-***-****

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MICROBIOLOGIST

Seeking challenging and growth oriented assignment commensurate with my knowledge and experience in Healthcare | Research & Development | Quality Control

PROFILE

Diligent and competent Microbiologist with 4.8 years of experience; presently working as Senior Research Fellow in Veterinary College Palampur.

A strong team player, training, guiding and motivating team members towards maximum productivity with exceptional consensus building, negotiation & interpersonal skills, analytical mind and comprehensive problem detection/ solving abilities.

Proven track record, with an ability to excel within a challenging environment, initiating and executing across a portfolio of projects harmonizing technical needs with business realities.

KEY SKILLS

• Testing Pathogens in Finish Products, Line Samples, Culinary Samples, Raw Materials and Environmental Sample

• Analysis & Confirmative biochemical tests of Bacteria (Salmonella, S.aureus, E.coli, Coliform, C.botulinum)

• Analysis of Food Organisms (Yeast and Mold, B.cereus, Listeria.monocytogenes, Shigella, Spores, C.botulinum, C.perfringens, Flat Sours, Vibrio cholera, Vibrio parahaemolyticus, Faecal streptococci)

• Burri and Enterobacteriacea (EB) Test

• Analyze Water Samples for Biological Impurities

• Most Probable Number (MPN) and Total Plate Count (TPC)

• Microbiological Techniques (Streaking, Pour Plating, Spreading, Stainings, Autoclaving, Media Preparation)

• Assessing the hematological parameters like Hb count, counting of neutrophils, Basophiles etc.

• Handling HPLC, GC, PCR, Spectrophotometer.

• Standardizing protocols for the experiment.(in-vivo and in-vitro)

• Isolations of field strains associated with skin and wound pathogens.

• Confirming the field strains upto species level by performing the biochemical tests.

• Lyophilisation of extracts and cultures isolated.

• Preparation and fractionating plant extracts.

• Isolation and identification of the bioactive compounds in plants.

• Knowledge of Cell culture.

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WORK EXPERIENCE

College of Veterinary Sciences, Palampur (H.P)

Senior Research Fellow | January 2009 till date

• Responsible for standardizing the protocols for antibacterial and antifungal activity of sea buckthorn seed oil and leaf extract.

• Isolating various organisms associated with skin and wound pathogens.

• Performance of the in-vitro experiment with standard and field isolates

• in-vivo experiment for determining the anti-bacterial activity, taking rabbit as the experimental model.

• Preparing and fractionating extracts from plant (leaves).

• Isolation and identification of the bioactive compounds in plant parts then studying their antimicrobial effect against the pathogenic clinical strains.

Institute of Microbial technology, Chandigarh (U.T).

Project Assistant | July 2008 to Dec 2008

• Responsible for carrying out the protocols related to Biodegradation of pesticides.

Nestle India Ltd., Moga Factory, Punjab (CQAL)

Microbiologist | October 2006 to September 2007

• Responsible for undertaking Dilution Preparation and Analysis as well as Providing MIS Data for Mini Business Unit (MBU) Meeting

• Preparing for Internal & External Audits to achieve Quality Standards as per the Process Benchmarks

• Responsible for conducting Tests under BIS (Bureau of Indian Standards)

• Charged with the daily closure of Samples

• Responsible for coordinating with Media Department for Daily Media Preparation

• Daily Checking and signing-off the pH of various prepared Microbial Media; Maintaining & Signing Off Stock Cards

• Planning, Assigning, Managing and Imparting Training (regarding Laboratory Techniques) to the Trainees

• Maintaining Hygiene, Good Lab Practices (GLP) and Safety within Lab Premise; conduct Quality Lab Hygiene Checks.

Attainments:

• Isolated many strains both fungal and bacterial from soil and clinical cases and preserved at -80 C(Bacterial) and fungal cultures under mineral oil at 4 C.

• Isolated Bacillus Species and standardized its growth parameters.

• Handled Spectrophotometer, HPLC,GC, PCR

• Isolated bacteria growing in soil fortified with pesticides (4,4 Dihydroxy biphenyle.) and Markfed Chandigarh.

• Experiments were done for determining the Biodegradation of soil containing Pesticides which contaminates the environment seriously

• Determined the Minimum Inhibitory Concentration (MIC) of Sea buckthorn seed oil and leaves.

• in-vitro and in-vivo experiment was done with clinical and standard strains and is still under progress with more no. of isolates.

• Culture sensitivity test (Bauer and Kirby) was done with different clinical isolates(bacterial and fungal).

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ACADEMIC QUALIFICATIONS

MSc. Microbiology; Gharwal University2006

Sardar Bhagwan Singh Post Graduate Institute of Bio-Medical Sciences and Research (S.B.S.P.G.I.), Dehradun 70%

BSc. Microbiology; Guru Nanak Dev University2004

Kanya Maha Vidyalaya, Jalandhar 68%

XIIth; Himachal Pradesh Board of School Education 2001

Senior Secondary School, Dharamshala 63%s

On Job Trainings

• ISO 17025 Training

• Training on handling/storing chemical (harmful, corrosive, flammable, in-flammable, toxic, poisonous)

• Fire Fighting Trainings

• First-aid Training

Projects Undertaken

Project Name I: Production of a Thermstable alpha-Amylase from Thermophilic Bacillus Species

During: MSc 4th Semester

Duration: January 2006 to June 2006

Project name II: Exploitation of India's Rich Microbial Diversity. (Biotransformation of Pesticides.)

Duration: July2008 to December 2008

Project name III: ‘in-vitro and in-vivo investigations on antimicrobial properties of sea buckthorn oil and formulations and leaf extract against common bacteria and fungi associated with skin and wound infections of animals.

Duration: January 2009 till date.

IT Proficiency : MS Office & Internet

Attendance in National and International Symposium

• Participated in one day Colloquium on “Animal diseases and Zoonoses in Northwestern Himalayas” organized by the Indian Society for Veterinary Immunology and Biotechnology (ISVIB) held at Department of Veterinary Microbiology, Dr.G.C.Negi College of Veterinary and Animal Sciences, Palampur, 19 September 2009.

• Participated in National conference on Sea buckthorn: Emerging Trends in Production to Consumption (February, 16-18, 2010) organized by CSK HPKV, Palampur, H.P.

• Participated in National conference on Seabuckthorn: Emerging Trends in R&D on Health Protection &Environmental Conservation (December 1-3,2011)

Seminar Delivered

Assessment of antibacterial activity of sea buckthorn (Hippophae rhamonides) seed oil against common skin pathogens.

Research Articles National and International

National:

1. Mandeep Sharma, Akanksha Palial, Rajesh Chahota, Harshit Verma. “Assessment of antimicrobial activity of Seabuckthorn seed oil against skin infections” Paper presented in the National Conference on Seabuckthorn: Emerging trends in Production to Consumption held at CSK HPKV, Palampur from 16.2.2010 to 18.2.2010.

2. Rajesh Chahota, Akanksha Palial, Harshit Verma and Mandeep Sharma “Evaluating antimicrobial properties of seabuckthorn (Hippophae rhamnoides L.) seed oil against strains of Staphylococcus spp. commonly involved in animal skin infections”. Paper presented in the Xth Indian Veterinary Congress and XVII Annual Conference of Indian association for the Advancement of Veterinary Research (IAAVR) and National Symposium on Challenges in Veterinry Reserch Education vis-à-vis safe animal, food and human health, held at College of Veterinary Science and Animal Husbandry, MP Pashu Chikitsa Vigyan Vishwa Vidyalaya, Jabalpur from 11.3.2010 to 12.3.2010.

3. Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virendra Singh “Comparative Antimicrobial Activities of Leaf Extracts of Hippophae rhamnoides and Hippophae salicifolia Species of Seabuckthorn against Bacterial Pathogens Associated with Skin and Wound Infections of Livestock of Himachal Pradesh”: ” Proceedings of National Conference on Seabuckthorn (Hippophae L.):Emerging Trends in R&D ON Health Protection & Environmantal Conservation.(December 1-3,2011)

4. Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virender Singh “Antibacterial activity of seabuckthorn Hippophae salicifolia leaf extract against skin and wound pathogens of domestic animals

International:

1. Mandeep Sharma Rajesh Chahota and Akanksha Palial

“Antimicrobial activity of sea buckthorn (Hippophae Rhamnoides l.) seed oil against staphylococcus aureus strains isolated from skin infections of animal.”: Seabuckthorn on the way between Sceience and Industry Interaction, Fourth International Seabuckthorn Association Conference ,Belokuriha, Russia September 1-6, 2009

2. Rajesh Chahota1, Mandeep Sharma1, Harshit Verma1, Akanksha Palial1, R.D. Patil2, Arvind Sharma3 and N. P. Kuradey4 “Studies on effects of seabuckthorn (Hipphophae L.) Leaf extract and seed oil on infected cutaneous wound healing process in rabbit experimental model”: Developing Seabuckthorn for the Harmonious Ecosystem & Healthy Life, Fifth International Seabuckthorn Association Conference(ISA- Sept 4-9,2011)

3. Mandeep Sharma1, Rajesh Chahota1, Harshit Verma1 and Akanksha Palial1 “Investigation of antibacterial properties of seabuckthorn (Hippophae rhamnoides L.) leaf extracts against common skin and wound microbial pathogens ”: Developing Seabuckthorn for the Harmonious Ecosystem & Healthy Life, Fifth International Seabuckthorn Association Conference(ISA- Sept 4-9,2011)

Full Papers Published:

1. Verma H, Chahota R, Palial A, Patil RD, Sharma A, Kuradey N.P, Sharma M. Effect of seabuckthorn (Hipphophae L.) Leaf extract and seed oil on infected cutaneous wound healing process in rabbit model. Indian Journal of Animal Sciences 82 (1): 54–00, January 2012

2. Verma H, Chahota R, Palial A, Sharma M. Studies on antibacterial properties of seabuckthorn (Hippophae rhamnoides L.) leaf extracts against common skin and wound microbial pathogens.. Indian Veterinary Research Journal (vol.20,No.1-2011)

3. Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virendra Singh “Comparative Antimicrobial Activities of Leaf Extracts of Hippophae rhamnoides and Hippophae salicifolia Species of Seabuckthorn against Bacterial Pathogens Associated with Skin and Wound Infections of Livestock of Himachal Pradesh” Proceedings of National Conference on Seabuckthorn (Hippophae L.):Emerging Trends in R&D ON Health Protection & Environmantal Conservation.(December 1-3,2011)

PERSONAL VITAE

Date of Birth : 17th February, 1983

Permanent Address : 270, Lower Shyam Nagar, Dharamshala, Kangra, Himachal Pradesh-176215.

Phone : 098********, 018**-******

References : Available on request

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