Microbiologist (molecular, fermentation, and project development)

Job Objective: A challenging Product Development/ Research Scientist position in an industrial company that utilizes my problem solving and innovative test developing skills.

Qualifications Overview:

• Molecular Biology: Plasmid, DNA, & RNA Extraction. DNA and RNA quantification using a Nanodrop system. PCR amplification (Real Time and Reverse PCR). Cytogenetics (PNA Probe Hybridization and Imaging).

• Proteomics: SDS Gel Electrophoresis, Protein Quantification, Protein Purification, Enzymatic Assays, Protein Precipitation, and Western Blotting.

• Cell Culture: Aerobic/ Anaerobic Cultivation (solid, liquid, and biphasic gas media conditions). Aerobic Fermentation, Cellular Respiration, and Cellular Inhibition Studies (1-3L Systems and Respirometers).

• Microscopic Imaging: Confocal Phase Contrast Imaging and Fluorescent Imaging.

• Experimental Development: Designed, Developed, and Analyzed data collected from Chemist, Engineers, and Environmental Scientist to write biweekly or monthly client reports.

Education/ Training:

Ph.D. of Biological Sciences (Environmental Microbiology),Georgia State Univ., Atlanta, Ga. 2011

Masters of Biological Science , Georgia State University, Atlanta, Ga. 2007

Bachelor of Science, Valdosta State University, Valdosta, Ga. 2003

Industrial Plants Safety and Regulation Training, E.B.S. Baton Rouge, LA 2011

CITI Training, Georgia State Univ., Atlanta, Ga. 2010

Biosafety and Bio-hazardous Training Georgia State Univ., Atlanta, Ga. 2009

GLP Training- Georgia State Univ., Atlanta, Ga. 2009

Radioactive Training Georgia State Univ., Atlanta, Ga. 2007

Awards and Publications:

1. Won “Best Research Poster Presentation” at SIM Conference (2011)

2. Co-patented dissertation research protocol with GSU. Focused on inducing hydrolyzing enzymes to delayed fruit ripening. (2011)

3. Wrote presentation “Understanding ARP (Active Cellular Respiration) Presentation”, for clients. Presentation taught at company seminar (Spring 2012) and sent to consultants of fortune 500 companies to help them understand fluorescent viability testing and the benefits to their company.

Relevant Work Experience:

E.B.S. Consulting Company (Sr. Microbiologist) -Mandeville, La. 10/11-Current

1. Developed and designed over 60% of the new advance microbiology testing at E.B.S. The company has 5 new testing techniques offer client’s to troubleshoot issues with wastewater systems.

2. Measured and analyzed cellular health of microbial population using cell respiration assays and fluorescent direct microscope counts.

3. Supervised lab technicians using fluorescent imaging and data compilation.

4. Tested and measured surfactant (anionic, cationic, and non-ionic) concentrations in waste waters.

5. Wrote and submitted detailed reports to clients that analyzed chemical and microscopic evaluations of clients waste water samples using InDesign and Excel software.

6. Wrote ARP “Active Respiring Percentage” Fluorescent Assay, Fluorescent Direct Count, and Nitrifying FISH Probe protocols.

7. The Sr. Microbiologist included 60% research/ client testing, 10% administrative work, 10% supervision, and 20% equipment ordering.

GSU GRA (Graduate Research Assistant) - Atlanta, Ga. 10/08-08/11

1. Designed and developed protocol that induces novel induction pathway for industrial enzymes in Rhodococcus rhodochrous bacterium. Experiment was co-patented by GSU.

2. Research focus was delaying fruit ripening by inducing target protein in Rhodococcus.

3. Academic research experience (including lab rotations): include 20% bioremediation, 10% genomic studies, 15% biocides/ microbial growth control, and 55% bacterial and plant interactions and cellular physiology.

Teaching Experience:

Gwinnett Technical College (Adjunct Instructor) – Lawrenceville, Ga. 09/09- 9/10

1. Taught Intro Biology & Microbiology laboratory and lectures.

2. Designed exams and PowerPoint presentations for students

3. Calculated and issued grades to students for the course.

GSU Bio-Bus GRA (Graduate Research Assistant) - Atlanta, Ga. 08/05-08/09

1. Taught science modules for students (pre-K to 12th grade).

2. Designed a high school chemistry module called “States of Matter”.

Computer Skills:

Operating Software: Unicorn- GE AKATA software, EasyChrom - GC software, BioCommand software – New Brunswick Fermentation Software, New Cell Dimension software- Purchased for Olympus Confocal Microscope Imaging and Cell Counting.

Business Software: Microsoft Excel, Word, Picture Manager, and Powerpoint programs.

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