Scientist / Manager Scientist

Yiming Ye, Ph. D.

**** ******** ****, ******, ** 30096

PH: 770-***-**** Email: ********@*****.***

U.S. CITIZEN

Summary of Qualifications:

1. Proteomics, Biology and Analytical Chemistry: Exceptional experience in analysis of proteins, peptides and PTMs using LC-ESI-TOF, ESI-QTOF, MALDI-TOF-TOF, 1D & 2D gel electrophoresis, 2D-DIGE and liquid chromatography. Expert in protein expression, protein purification, gel filtration, ion exchange chromatography, peptide mapping, surface plasmon resonance, microfiltration, PCR, cell culture, cloning, mutagenesis techniques. Extended skills in Western Blot, ELISA, CE, mass spectrometry, FTIR/Raman, TLC, VIS/UV/IR spectrophotometers, optical microscopy, NMR, HPLC, FPLC, ITC, Biacore, DSC, fluorescence and circular dichroism spectrometry.

2. Computer programs: Proficient in the use of Mascot, DeCyder, Peaks, Image Master, PD Quest, vector NTI suite software and molecular modeling (Rasmol, Molscript and Sybyl), analytical instrument software, Microsoft Word, Access, Excel and PowerPoint, as well as applicable data analysis software.

3. Other: Excellent time management. Solid written and oral communication skill. Experience in writing and reviewing SOP and GTR documents. Attention to detail in documentation practices. Prove experience in training graduate students, postdoctoral fellows, and technicians. Demonstrated ability to execute several projects simultaneously.

Education:

Ph.D., Biochemistry, Georgia State University, Atlanta, GA. Advisor: Dr. Jenny J. Yang

M.S., Microbiology. Jinan Univ., Guangzhou, China. Advisor: Dr. Shantu Sun

B.S., Biology, Jinan Univ., Guangzhou, China. Advisor: Dr. Yaojaun Zheng

Research Experience:

Jan. 2005 to Dec. 2010 Chemist, Proteomics Laboratory, Biotechnology Core Facility Branch, Centers for Disease Control and Prevention (CDC), Atlanta.

Develop and apply proteomics techniques for protein characterization in infectious disease research.

Research projects:

1. Protein biomarker discovery for the early detection of human cervical cancer by 1D & 2D electrophoresis and mass spectrometry.

2. Investigation on proteins related to Kawasaki disease using PCR, Western blot, electrophoresis TOF and LC-MS/MS.

3. Identification of differentially expressed Proteins In dendritic cells treated with Chlamydia Trachonatis by 2D-DIGE technique, TOF and LC-MS/MS.

4. Thermal stability study of recombinant protective antigen of Anthrax lethal toxin using electrophoresis, HPLC, circular dichroism and fluorescence.

5. Development of convalescent sera-assisted antigen capture and antigen detection method for pathogen discovery

6. Mapping epitopes for monoclonal antibodies specific to monkey pox virus using western blot, ELISA, Biacore protein interaction technique and mass spectrometry

7. Formulation, pharmacokinetics and biodistribution of Ofloxacin loaded albumin microparticles and nanoparticles by HPLC

Dec. 2001 to Jan. 2005 Postdoctoral Research Associate, Department of Chemistry, Georgia State University, Atlanta, GA. Advisor: Dr. Jenny J. Yang

Research Projects:

1. Design of Calcium Binding Proteins with medical applications by using PCR, cloning, mutagenesis, protein expression and purification, NMR, circular dichroism and fluorescence.

2. Designed protein-based MRI contrast agents for molecular imaging of cancers

3. Developed calcium and proteinase sensors

4. Understanding virus infection

May 1997 to Dec. 2001 Doctorate Graduate Research, Department of Chemistry, Georgia State University, Atlanta, GA. Advisor: Dr. Jenny J. Yang

Dissertation: A Grafting Approach to Obtain Site-Specific metal-binding properties of EF-hand proteins

Awards and Honors

2001 First Prize of Poster Competition in 9th Suddath Symposium, Atlanta, GA

2000 First Prize of Poster Competition in 8th Suddath Symposium, Atlanta, GA

1999 First Prize of Poster Competition in 51th Southeastern Regional Meeting of the American Chemical Society, Knoxville, TN

1998 First Prize of Poster Competition in 10th Annual Frederick and Mapp Symposium, GA

Training

2005 Proteomics: Cold Spring Harbor Laboratory

Patent:

Title: Analyte sensors, methods for preparing and using such sensors, and methods of detecting analyte activity.

Publication date: 08/05/2010

Patent application number: 201********

Publications

1 Kemba Lee, Martin D'Souza, Yiming Ye, Janice Carr, Kevin Karem (2011), Formulation, pharmacokinetics and biodistribution of Ofloxacin loaded albumin microparticles and nanoparticles, Journal of Microencapsulation (In press)

2 Yiming Ye, Gitika Panicker, Elizabeth Unger and Dongxia Wang (2010), Characterization of Human Cervical Mucous, Proteins Clinical Proteomics 2010 June; 6:18-28.

3 Lixia Wei, Shunyi Li, Jianhua Yang, Yiming Ye, Jin Zou, Liya Wang, Jenny Yang and Zhiren Liu (2010), Protein-based MIR contrast agents for molecular imaging of prostate cancer, Molecular Imaging & Biology (2010).

4 Yubin Zhou, Wen-Pin Tzeng, Yiming Ye, Yanyi Chen, Yun Huang, Teryl Frey, Jennt Yang (2010), Calcium-dependent association of calmoduling with the Rubella virus nonstructural protease domain, J. Biol. Chem., 2010 Jan. 19

5 Yiming Ye, Eng-Chun Mar, Suxiang Tong, Scott Sammons, Sunan Fang, Larry J. Anderson and Dongxia Wang (2010), Application of proteomics methods for pathogen discovery. Journal of Virological Methods, 2010 Jan;163(1):87-95

6 Ning Chen, Yiming Ye, Jin Zou, Shunyi Li, Siming Wang, Amy Martin, Robert Wohlhueter, Jenny Yang (2009), Fluorescence complementation via EF-hand interactions. J Biotechnol, 142(3-4):205-13

7 Ning Chen, Jin Zou, Siming Wang, Yiming Ye, Yun Huang, Gadda G, Jenny Yang (2009), Designing protease sensors for real-time imaging of trypsin activation in pancreatic cancer cells. Biochemistry, 48(15):3519-26

8 Yubin Zhou, Wen-Pin Tzeng, Yiming Ye, Yun Huang, Shunyi Li, Yanyi Chen, Teryl K Frey, Jenny Yang (2009) A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication. Biochem J. 417(2):477-83

9 Yubin Zhou, Wei Yang, Monica Lurtz, Yiming Ye, Yun Huang, Hsiau-wei Lee, Yanyi Chen, Charles Louis, and Jenny Yang. (2007), Identification of the Calmodulin Binding Domain of Connexin43. Journal of biological chemistry, 282(48):35005-17

10 Yubin Zhou, Wen-Pin Tzeng, Wei Yang, Yumei Zhou, Yiming Ye, Hsiau-wei Lee, Teryl K. Frey, and Jenny Yang. (2007), Identification of a Ca2+-Binding Domain in the Rubella Virus Nonstructural Protease. Journal of Virology, 81, 7517-28

11 Amy Carroll, Yiming Ye, Wei Yang, Robert Simmons, Jenny J. Yang. (2006) Amyloid Fibril Formation by a Domain of a Rat Cell Adhesion Molecule. Cell Biochemistry and Biophysics. 44(2):241-249

12 Yiming Ye, Hsiau-Wei Lee, Wei Yang and Jenny J. Yang (2005) Calcium and Lanthanide Affinity of the EF-loops from the C-terminal of Calmodulin. Journal of Inorganic Biochemistry. 99 (6), 1376-1383

13 Yiming Ye, Hsiau-Wei Lee, Wei Yang, Sarah Shealy, and Jenny J. Yang. (2005) Probing Site-Specific Calcium and Lanthanide Binding Affinity of Calmodulin by Grafting. Journal of the American Chemical Society, 127 (11), 3743 -3750

14 Jin Zou, Yiming Ye, Kristy Welshhans, Monica Lurtz, April Ellis, Charles Louis, Vincent Rehder and Jenny J. Yang (2005) Expression and Optical Properties of Green Fluorescent Protein Expressed in Different Cellular Environments. Journal of Biotechnology. Jun; 99(6):1376-83.

15 Wei Yang, Anna L. Wilkins, Shunyi Li, Yiming Ye, and Jenny J. Yang (2005) The effects of Ca2+ binding on the dynamic properties of a designed Ca2+-binding protein. Biochemistry. 44 (23), 8267 -8273

16 Wei Yang, Anna L. Wilkins, Yiming Ye, Zhi-ren Liu, Shun-yi Li, Jeffrey L. Urbauer, Homme W. Hellinga, Alice Kearney, P. Anton van der Merwe, and Jenny J. Yang. (2005) Design of a Calcium-Binding Protein with Desired Structure in a Cell Adhesion Molecule. Journal of the American Chemical Society, 127 (7), 2085 -2093

17 Wei Yang, Lisa Jones, Leanne Isley, Yiming Ye, Hsiau-Wei Lee, Anna Wilkins, Zhi-ren Liu, Homme W. Hellinga, Russell Malchow, Mohammed Ghazi and Jenny J. Yang. De Novo Design of a Calcium Binding Protein. Journal of the American Chemical Society, (2003). 125(20); 6165-6171

18 Yiming Ye, Sarah Shealy, Hsiau-Wei Lee, Ivan Torshin, Robert Harrison and Jenny J. Yang (2003) A grafting approach to obtain site-specific metal binding properties of EF-hand proteins. Protein Engineering 16(6) 429-434

19 Jenny J. Yang, Amy Gawthrop, and Yiming Ye (2003) Calcium binding Affinity and Selectivity of calmodulin, Protein and peptide Letter 10(4): 331-345

20 Yiming Ye, Hsiau-Wei, Wei Yang, Sarah J. Shealy, Anna L. Wilkins, Zhi-ren Liu, Ivan Torshin, Robert Harrison, Robert Wohlhueter and Jenny J. Yang. (2001) Metal binding affinity and structural properties of an isolated EF-loop in a scaffold protein. Protein Engineering 14(12): 101-113

21 Yiming Ye, Annie Lin and Jenny J. Yang (2001) Calcium binding properties of EF-loops in a beta-sheet protein. Peptides: The wave of the future, R. A. Houghten & M. Lebl. (Eds.), Escom Leiden.

22 Anna L. Wilkins, Yiming Ye, Wei Yang, Zhi-ren Liu, Sarah J. Shealy, Hsiau-wei Lee and Jenny J. Yang (2001) Calcium binding affinity of a de novo designed protein. Protein Engineering 15(7): 571-574

23 Hsiau-wei Lee, Wei Yang, Yiming Ye, Zhi-ren Liu, John Glushka and Jenny J. Yang (2001) Isolated EF-loop of calmodulin in a scaffold protein remains monomeric in solution. BBA 1598: 80-87

24 Jenny J. Yang, Yiming Ye, Amy R. Carroll, Wei Yang and Hsiau-wei Lee. (2001) Structural biology of the cell adhesion protein CD2: Alternatively folded states and structure-function relation. Current protein and peptide science, 2(1): 1-17

25 Jenny J. Yang, Haidong Yang, Yiming Ye, Harry Hopkins and Gary Hastings (2001) Non-native intermediate of an all beta-sheet protein: domain 1 of CD2. Cell Biochemistry and Biophysics, 36: 1-17

26 Jenny J. Yang, Amy R. Carroll, Wei Yang, Yiming Ye and Christina Nguyen (2000) Cell Biochemistry and Biophysics 33: 253-273

27 Wei Yang, Yiming Ye and Jenny J. Yang (1999) A sensitive method for selecting calcium binding protein Luminescene Forum 5:4-8

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