Engineer Process

Xueli (Shirley) Wang

U.S.A. Permanent Resident

Home address: *** **** ** ****** ** 02464

Email: ****************@*****.***.**

Cell: 857-***-****

Professional Overview: Results-oriented and self-driven research associate with broad range of skills in molecular biology, immunology, cell biology.

Core qualifications:

• Cellular Biology:

Cell culture, cell-based assays, si/shRNA transfection, multi-color Flow cytometry.

• Molecular Biology and Protein Biochemistry

• DNA/RNA isolation, PCR, RT-PCR, and Real-time RT-PCR, plasmid NDA construction, cloning, Northern Blot, protein expression and purification, SDS-PAGE, Western blot, protease enzymatic assay, ELISA.

• Microbiology and Fermentation:

Both aerobic and anaerobic culture, fermentation and harvest process.

• Animal model and handling:

Breeding, weaning, anesthesia, animal surgery, organ and blood collection, dosing techniques (IP, IV, PO, SC), brain dissection.

Education

Bachelor in Biology/Microbiology 1991-1995

University of Science & Technology of Tianjin, China

Experience

Research Associate, Molecular Biology Group

Qteros, Inc. Marlborough, MA Oct. 2010-Dec.11.2011

Worked on improvement of fermentation performance by modifying metabolic enzymes.

• Generated bacterial strains with modified metabolic enzymes: Successfully generated a series of plasmids with specific integration sites using E.coli expression system. Developed a series of clostridia stain increase ethanol production.

Successfully screened the integration in targeted region.

• Conducted fermentation experiments: Executed anaerobic shaking flasks and small scale fermentations.

Research Associate

Division of Rheumatology, Immunology and Allergy

Brigham and Women’s Hospital Feb.2005-Oct.2010

• Cell cultures and co-culture: Generated and cultured cells from mice bone marrow or human cord blood; cultured dendritic cells and fibroblast cells culture, insect cells, mammalian cell culture and others; generated some cell lines; suppression co-culture mast cells and fibroblast.

• Cell line transfect ion, cytokine generation and detection: siRNA/shRNA (Mast cell proteases) cloned to Lentivirals vector and transected into LAD2 cells (gene knock out). Protease-induced cytokine generation detected by real-time RT-PCR or ELISA in endothelial cells, and fibronectin cleavage by protease deficient mast cell proteases.

• Protein generation, purification and enzymatic assays: Expressed different mast cell proteases using Baculovirus vectors in insect cells or mammalian cells. Purified recombinant proteases using anti-Flag column. Activated inactive recombinant proteases using enterokinase, performed enzymatic assays.

• Animal models: Successfully completed DSS-induced colitis (chemically induced inflammation), Derf extract-induced pulmonary inflammation, the role of mast cell proteases in angiogenesis in tumor studies.

Fermentation/bioprocess Engineer

Pharmaceutical company of Norman Bethune Medical University, China 1995-2003

• Served as Process Engineer for commercial fermentation.

• Supported fungi and microbial cell culture (media preparation and formulation) and harvest.

• Provided real time troubleshooting for unit operations (cell culture, high throughput fermentation and cGMP purification).

Reference

Matthias Schmalisch

Scientist Molecular Microbiology and Bioinformatics

Email: ********.**********@*****.*** Phone:617-***-****

Matthew J Hamilton MD.

Instructor in Medicine

Brigham and Women’s Hospital Harvard Medical School

Email: *********@********.***

Patrick O’Mullan

Director of Molecular and Microbiology at Qteros,Inc

Email: *******.*******@*****.***

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