Project Model

* ********** **., ******** *****, NJ, ***** Tel: 201-***-**** Email: ********@*****.***

Libin Li, Ph.D.

Education

1999–2006 Rutgers University and University of Medicine and Dentistry of New Jersey, Newark, NJ

Ph.D., Neurology and Neuroscience.

1991–1995 Nanjing University Nanjing, China

B.S., Biology.

Experience

2008-Present University of Medicine and Dentistry of New Jersey Newark, NJ

Post-Doctoral Fellow

Studied immuno-pathogenesis and Biomarkers of multiple sclerosis (MS) using an animal model of demyelination and disability in mice with theiler’s murine encephalomyelitis virus (TMEV) infection.

Completed a study to evaluate FTY720 (“fingolimod” in a phase III program “FREEDOMS”) in Theiler’s MS model for Novartis Pharmaceuticals; paper published.

Completed a study to evaluate therapeutic effect of Rituximab (anti-mouse CD20 (5D2)) in viral model of MS for Roche; paper submitted.

Leading an ongoing project to study therapeutic effect of Teriflunomide in viral model of MS for Sanofi Aventis.

Developed a novel technology of rapid cloning of recombinant monoclonal antibody from single plasma cell to study antigenic specificity of antibody production in viral model of MS.

Developed a Luciferase-assay kit for measuring neutralizing antibody in MS patients for Biomonitor.

Investigating molecular basis of axon damage and remyelination using Laser capture and Deep Sequencing.

2006-2008 Shanghai Bio-Serve Biotechnology Co. LTD., Shanghai, China

Industry experience

Serve as Senior Principal Scientist in the company.

Manage the establishment of new company division in Baiqiao Biological Park, Wuxi, China.

Engagement of project introduction and investment from America to Industrial Zones in Jiangsu, China.

1999-2006 University of Medicine and Dentistry of New Jersey, Newark, NJ

Ph.D. Candidate

Established a mouse model of Lyme neuroborreliosis using the causative agent, borrelia burgdorferi..

Identified the mechanisms of intrathecal antibody formation after central nervous system (CNS) infection to provide insight into the pathogenesis of Lyme disease and MS.

1995–1998 Cancer Research Laboratory of Shanghai First Pulmonary Hospital Shanghai, China

Assistant Scientist

Developed monoclonal antibody conjugated anti-tumor drugs in lung cancer therapy.

Participated in a clinical trial of Lymphokine-activated killer (LAK) therapy for lung cancer patients.

1994–1995 Shanghai Institute of Cell Biology, Chinese Academy of Sciences Shanghai, China

Internship

Developed a new method of clone selection with Sodium alginate in constructing the yeast artificial chromosome (YAC) cDNA library for the Chinese genome project.

Key Skills

Laboratory Research:

Molecular Biology: Real-time PCR (Taqman); Gene cloning; Deep sequencing (SOLiD™ 3); In vitro hybridization; Expression of recombinant monoclonal antibody from single cell, Northern blot; In situ hybridization.

Cell Biology: Cytopathatic assay for detection of neutralizing antibodies; Flow cytometry; Cell purification; Cell culture; Laser capture; Microscope; B-cell assays; Cytokine assays; IFNß bioactivity assay.

Immunology: ELISA, ELIspot; Immunoblotting; Immunohistochemistry.

Virology: Theiler’s murine encephalomyelitis virus (TMEV) culture; Primary TMEV amplification after in-vivo growth in the CNS; Viral plaque assay; Virus RNA copy cloning.

Neurology: Primary culture of spinal cord; Neurite outgrowth in neuroblastomas; Axonal quanititation; Electronic measure of potentials; Mouse CSF isolation;

Protein Chemistry: Luminex; Isoelectric focusing (IEF); Western blot.

Animal Model: Mouse model of multiple sclerosis (MS) induced by theiler’s murine encephalomyelitis virus; Mouse and monkey models of Lyme neuroborreliosis induced by borrelia burgdorferi; Experienced in laboratory animal handling and manipulations.

Bioinformatics: Applications of public and proprietary sequence databases; homology modeling; sequential alignment and structural searching, docking, and molecular dynamics applied to drug design.

Awards

Bioinformatics Institute of UMDNJ and Rutgers University, Bioinformatics Certificate Program fellowship (2002-2004)

Travel award for Whitaker Prize at CMSC conferences (2004).

Invited Talk

• Analysis of intrathecal antibody production in a mouse model of neuroborreliosis. Whitaker Research Track of 18th annual meeting of the Consortium of Multiple Sclerosis Centers (CMSC). June 2-6, 2004 Toronto, Canada.

• Investment and entrepreneurship in Industrial Development Zone in China. Google Groups "ChinaTalentPub” Annual conference. July 10, 2010, Columbia University, USA

Publications

1. Li L, Matsumoto M, Pachner AR., The Effect of B-cell depletion in the Theiler's viral Model of Multiple Sclerosis. Submitted.

2. Li L, Matsumoto M, Seabrook TJ, Cojean C, Brinkman V, Pachner AR., The Effect of FTY720 in the Theiler's viral Model of Multiple Sclerosis. J Neurol Sci, 2011 Sep 15;308(1-2):41-8.

3. Pachner AR., Li L, Lagunoff D. Plasma Cells in the Central Nervous System in the Theiler’s Virus Model of Multiple Sclerosis. J Neuroimmunol. 2011,March, 232(1); 35-40.

4. Pachner AR, Li L, Narayan K. Intrathecal antibody production in an animal model of multiple sclerosis. J Neuroimmunol. 2007 Apr; 185(1-2):57-63.

5. Li L, Narayan K, Pak E, Pachner AR Intrathecal antibody production in a mouse model of Lyme neuroborreliosis. J Neuroimmunol. 2006 Apr; 173(1-2):56-68.

6. Narayan K, Dail D, Li L, Cadavid D, Amrute S, Fitzgerald-Bocarsly P, Pachner AR. The nervous system as ectopic germinal center: CXCL13 and IgG in lyme neuroborreliosis. Ann Neurol. 2005 Jun;57(6):813-23.

7. Pachner AR, Dail D, Li L, Gurey L, Feng S, Hodzic E, Barthold S. Humoral immune response associated with lyme borreliosis in nonhuman primates: analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins. Clin Diagn Lab Immunol. 2002 Nov;9(6):1348-55.

8. Wu G, Lu ZH, Xie X, Li L, Ledeen RW. Mutant NG108-15 cells (NG-CR72) deficient in GM1 synthase respond aberrantly to axonogenic stimuli and are vulnerable to calcium-induced apoptosis: they are rescued with LIGA-20. J Neurochem. 2001 Feb;76(3):690-702.

9. Tan L., Li L., Hong Q., Su, B.. The selective concentration of sulphadiazine-induced isotype in malignant. Tumor (Shanghai) November 1997, Vol.17 (6):470-472.

Abstracts

1) Andrew R. Pachner, Libin Li and Makiko Matsumoto. B-cell depletion abolishes anti-CNS autoantibody production in the Theiler’s virus model of Multiple Sclerosis . Abstract for 5th Joint triennial congress of the European and Americas Committees for Treatment and Research in Multiple Sclerosis, (Amsterdam, The Netherlands), 2011

2) Libin Li, Makiko Matsumoto, and Andrew Pachner. The effect of B-cell depletion in Theiler's viral model of Multiple Sclerosis. Abstract for 98th annual meeting of The American Association of Immunologists, ( San Francisco, CA). The Journal of Immunology, 186, 107.17. 2011.

3) Andrew R. Pachner, Libin Li, Makiko Matsumoto. The Effect of FTY720 in an Animal Model of MS. Abstract for 62th Annual Meeting of American Academy of Neurology (AAN).(Toronto, Canada), 2010

4) Andrew R. Pachner, Libin Li, Jennifer Brady, Priyanka Apte. Fingolimod (FTY720) reduces disease progression, but does not exacerbate viral load in Theiler's murine encephalomyelitis virus-induced demyelinating disease model of multiple sclerosis . Abstract for 25th Congress of the European Committee for the Treatment and Research in Multiple Sclerosis ( ECTRIMS), (Dusseldorf, Germany), September, 2009

5) Libin Li, Jennifer Brady, David Lagunoff and Andew Pachner. Characterization of CNS antibody-secreting cells, the blood-brain barrier, and autoantibody in the Theiler's model of MS. Abstract for 96th annual meeting of The American Association of Immunologists (AAI), ( Seattle, Washington). The Journal of Immunology, Abstract #361, program 137.7, 2009.

6) Libin Li, Kavitha Narayan, Elena Pak and Andrew R. Pachner. Analysis of Intrathecal Antibody Production In A Mouse Model of Neuroborreliosis, Abstract of the Annual Meeting of American Association of Immunologists (AAI), (San Diego, CA), Program of 563.17, 2005.

7) Libin Li and Andrew R. Pachner. Analysis of intrathecal antibody production in a mouse model of neuroborreliosis. Abstract and presented on the Whitaker Research Track of 18th annual meeting of the Consortium of Multiple Sclerosis Centers (CMSC). (Toronto, Canada). International Journal of MS Care. Abstract # w3,p86 2004.

8) Libin Li, Dail D, Kavitha Narayan and Pachner AR. A mouse model of lyme Neuroborreliosis: Comparison of American and European Strains of b.burgdorferi. Abstract of the 128 Annual Meeting of American Neurological Association (ANA) (San Francisco, CA), Annals of Neurology, Volume 54, Issue S7, 2003.

Reference

Available upon request.

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