Scientist/Chemist

Anshu Sharma

Ph 818-***-****)

mailto:********@***********.***

OBJECTIVE – To obtain a challenging position within the areas of Biochemistry and Biological Sciences.

Education

Masters of Science - Cell & Molecular Biology - St. Cloud State University, Minnesota. GPA 3.91

Bachelor of Science - Chemistry, Zoology & Botany – University of Rajasthan, India Aug 2000 (GPA 3.55)

UPPER LEVEL COURSES TAKEN

Advance Protein Techniques Research Design Advance Topic in Genetics

Advance DNA Technique Structure Based Drug Design

Introduction of Crystallography Applied Structural Genomics

Advance Cell and Tissue Culture Technique Seminar in Biology

Technical Skills

Western Blot

Co-immunoprecipitation

Media Preparation & testing sterility

Passaging Cells

Cell Cycle Arrest

Freezing & Thawing cells

Transient transfection by Calcium Phosphate precipitation

Liposomal DNA transfection

Flow Cytometry

Molecular Biology / DNA Lab Techniques

Bioinformatics and Computational Biology.

Bioinformatics Skills Databases Nucleotide GenBank, Pfam, COG, KEGG, Entrez (NCBI), BLAST suite, FASTA, CLUSTALW

Protein Assay, Enzyme Assay

Oligo Design

PCR

Restriction Digest

Agarose gel electrophoresis of DNA

Gateway cloning

Transformation of E. coli

Ion exchange and affinity chromatography.

SDS-PAGE

Trypsin Digest.

LCMS analysis of fragments

Hanging and sitting drop crystallization

Crystal seeding techniques, cryo-stabilization of crystals, mounting crystals.

X-Ray diffraction of protein crystals

Interpretation of X-Ray data

Research Experience

Dr. Bruce Jacobson Jan 2008 – Aug 2010

Discovery and Characterization of aP2 Inhibitors using X-ray Crystallography

Expression & Purification of Adipocyte fatty acid binding protein.

Co-Crystallization of aP2 protein with Ibuprofen

Electron Density Map Interpretation - Model Building

Data Collection. Structural Refinement – Validation and Structural Analysis

Projects Undertaken in Masters

Purification of Beta-Lactamase from E.coli

The First Annotation of the Histidine Biosynthetic pathway of Ammonifex Degensii

Differentiation of C2C12 cell line for myotubule formation

Calcium Phosphate mediated gene transfer into HEK 293T cell line

Determination of the Regulation of PGC-1 β a Transcriptional Activator by Ubiquitin Ligase Cdc4 γ in HEK 293T cells

TEACHING EXPERIENCE

Course/Year Faculty/University Responsibilities

Teaching Assistant for Human Biology (Spring 2008)

Dr. John C. Cornell

St. Cloud State University

St. Cloud. MN

Proctored students during the laboratory period and answered questions.

Set up the laboratory equipments and graded lab reports.

Teaching Assistant for Cell Function and Inheritance (Summer 2008)

Dr. Julius Matthew

Dr. Maureen L. Tubbiola

St. Cloud State University

St. Cloud. MN

Lectured on biology experiments and supervised students in the lab

Set up the laboratory equipments; prepared solutions, buffers and autoclaved equipments for aseptic work for laboratory use

Guided student on writing lab reports

Graded lab reports

Teaching Assistant for Cell Function and Inheritance (Fall 2008, Spring 2009 and Summer 2009)

Dr. Christopher A. Kvaal

Dr. Maureen L. Tubbiola

Dr. Michelle Wagner

St. Cloud State University

St. Cloud. MN

Extra Curricular /Achievements

Award for the best classical dancer

Participated in Poster making and science exhibitions

References – Provided upon request

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