Molecular Biologist/Biochemist

Shanmugapriya Selvaraj

*** ****** **** ****, **** Haven, CT 06516

Mobile: 860-***-**** Email: ********@*****.***

Education 09/09-01/11

University of New Haven: West Haven CT, USA

College of Arts and Sciences

Master of Science: Cellular and Molecular Biology

Bharathidasan University: Tiruchirapalli, India 07/03-05/05

Department of Biochemistry

Master of Science: Biochemistry

University of Delhi: New Delhi, India 07/00-05/03

Department of Chemistry

Bachelor of Science: Industrial Chemistry

Related Courses

Genomics Cell and Molecular Biology Immunology

Biochemistry Biomolecules Human Physiology and Nutrition

Cell and Tissue Culture Biophysical and Analytical Chemistry Genetics and Clinical Biochemistry

Biostatistics Microbial and Plant Biochemistry Hormones and Signals

Bioinformatics Enzymology and Metabolism Genetic Engineering and Biotechnology

Highlights of Qualifications

• Well experienced as Production Biochemist with wide knowledge in Biology, Biochemistry,

Molecular Biology and instrumentation techniques.

• Huge knowledge of cGMP regulations with GMP.

• Excellent analytical skills.

• Superior communication skills with the ability to follow instructions and protocols.

• Ability to coordinate with team members and higher ordinates as well as work in a team.

• Member, American Association of Biochemistry and Molecular Biology

Professional Experience

Molecular Biochemist 05/05 - 07/09 Department of Oncology and Pharmacology

Madras Pharmaceuticals Pvt. Ltd, Chennai, India

Responsibilities:

• Genetic Screening and studies

Shared my subjective expertise views and experience in genetic screening with siRNA/shRNA/ cDNA by conventional and viral transduction technologies.

DNA/RNA/protein isolation and characterization and cloning DNA, DNA sequencing using MPSS and Roche/454 Pyrosequencer.

Designing cell-based and biochemical assays.

Designing and random primer labeling of DNA probes Recombinant DNA technologies.

Generated recombinant retrovirus and lentivirus and transduction, and RNA interference – both siRNA and shRNA based knockdown

Performed transfection using viral transduction technologies and RNAi techniques.

Performed DNA cloning, Real-time PCR, observed protein-protein interaction, enzymatic and proliferation assays, high content imaging.

• Cell culture

Supplemented thoughts to peer in in-vitro pharmacology experimentation techniques including MTT cell proliferation/apoptosis and related functional/toxicity assays, reporter-based assays, and mammalian cell culture

Monitored cytotoxic studies. Ensured successful culture using efficient sterilization techniques.

Handled cancer cell lines such as Her2, HeLa, HL60, and cryopreservation of stable cell line.

Designed and analyzed cell-based assay for analysis of pathway.

Monitored the state of signaling molecules in cellular extracts.

Flow cytometry analysis (FACS) and cell uptake/internalization.

Centrifugation, enzyme activity assays, titration, ultrafiltration techniques, homogenization.

• Research Support

Decision-maker and team lead for a group of four Jr. Biochemists.

Purified and isolated active herbal anticancer formulatory-drugs, proteins, amino acids and peptide sequencing.

Instrumental set up using UV-spec, IR-spec, HPTLC, GC-MS, PCR, master cycler gradient for PCR, 7300 real-time PCR system (Applied Biosystems), autoclaving.

• Microscopy and Spectroscopy

Confocal and fluorescence microscopy (Leica DFC-500 microsystems), Nikon Eclipse H550L (80L), Dark field Microscopy, Phase-contrast microscopy.

UV-spectroscopy, IR-spectrometry, GC-MS, HPTLC, Electrophoresis, spectrophotometry.

• Team-working, analysis, and assistance

Worked independently and as a team to plan and execute experiments, and interpret results, made clinical observations, live-animal imaging, and harvested specimen for molecular and histopathological analysis

Monitored and performed qualitative and quantitative analysis of allopathic and herbal formulated drug, and protein purification and crystallization by hanging drop technique.

Performed analysis of water content (Kf), dissolution, weighing and standardizing chemicals and buffers

Responsible for preparation and documentation of research work, and data log maintenance

Research Projects

• In vitro susceptibility of different morphological forms of Borrelia burgdorferi to macrolides

09/10 - 01/11

University of New Haven, Department of Biology and Environmental Sciences, CT

Description: Evaluation of various macrolides and their effects on spirochetes, cysts, and biofilms of Borrelia burgdorferi based on their MBCs and MICs.

Highlights: DNA extraction and isolation, cell-culture bioassays, PCR, flow-spectrometry, BacLight staining, Western blotting, and ELISA. Wrote validation protocols, SOP, and reports.

• Carcinoembryonic antigen (CEA) as a biomarker in colorectal cancer 10/04 – 03/05

Bharathidasan Unviersity, Department of Biochemistry, India (Institute: G. Kuppuswamy Naidu Memorial Hospital, Coimbatore, India)

Description: Investigated and studied CEA as a primary biomarker in poorly, moderately, and highly differentiated adenocarcinoma of the colon using strong membranous and cytoplasmic staining with mAB Col-1.

Highlights: Cobas Integra 400, Roche Diagnostics; Elecsys 1010, Roche Diagnostics; Coulter

Counter

Skilled Techniques

• Molecular Biology/ Industrial Biotechnology techniques: Protein purification, liquid

chromatography (gel filtration, hydrophobic interaction, affinity, ion exchange chromatography), PCR, Biotek FLx 800 fluorescence Microplate Reader, ELX-800 Universal microplate reader, and Nanodrop 3300 Thermoscientific, microarrays, FISH testing.

• Immunology techniques: Immunoprecipitation, immunofluorescence, ELISA, Southern blot,

Northern blot, and Western blot, SDS-PAGE, immuno-detection, Biotinylation of proteins and oligopeptides, labeling of antibodies by colloidal gold, radioimmunoassay, radiolabeling of antibodies.

• Animal Handling: Handled and dosed rats and mice

• Chemistry: Dissolution, weighing and standardizing chemicals, titration, fractionation, distillation,

NMR.

Volunteer Work

• Secretary/Treasurer of Lyme Disease Research Club, University of New Haven, CT (2010)

• Participated in Lyme Disease concert at University of New Haven, CT (2010)

• Participated in Laboratory Safety seminars and Classes at University of New Haven , CT (2010)

• Organizer Team member - Breast Cancer awareness Program, G. Kuppuswamy Memorial Hospital,

Coimbatore, India. (2005)

• Participated in annual workshop, “Technological advancements in the field of Gene Cloning and

Immunological Techniques” by Department of Biotechnology, Government of India. (2005)

• Attended workshops conducted by Mother Dairy farm New Delhi, India. (2002)

Honors

Academic - University rank holder (20th), Bharathidasan University, India, 2005

Computer Skills

NCSS, MVSP, R program, SQL, PERL, Microsoft Office, C++, VB, UNIX

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