Assistant Engineering

HARSHAVARDHAN DESHMUKH

*** ********* ******, ********* ***, Ruston LA 71270

*********@*****.*** 318-***-**** (mobile)

Seeking a full time opportunity as a Research Assistant/Associate. No geographical preference – willing to relocate

SUMMARY

• 2+ years of experience in cell culturing, maintaining cell lines, fluorescence microscopy

• 2+ years of experience in electroporation, Transfection of mammalian cells

• Experience in PCR, ELISA, Gel Electrophoresis

• Worked on animal (cowhead) to isolate tissue and cells

• Effective team player plus an independent contributor

• Hardworking, ambitious, goal oriented, optimistic

EDUCATION

Louisiana Tech University, Ruston, LA, U.S.A Sep’09 to Nov’11

Master of Science, Molecular Science and Nanotechnology

Thesis Track, Graduate Advisor: Dr. Shengnian Wang

Graduation: March 2012, GPA: 3.72/4.0

Course Highlights: Principles of Cell and Molecular Biology, Induced Pluripotent Stem Cells

Research Methods, Histology, Cancer Biology, Advanced Immunology

Instrumental Analysis

Rai Foundation College, YCMO University, India Aug’03 to Nov’07

Bachelor of Technology, Biotechnology

GPA: 3.2/4.0

Course Highlights: Cell and Molecular Biology, Fermentation Processes, Pharmacology, Biochemistry,

Immunology, Physiology, Biostatistics and Food Engineering

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PROFESSIONAL EXPERIENCE

Graduate Research Assistant Dec’09 to Nov’11

Institute for Micro-manufacturing, Louisiana Tech University

Duties included: Enhance the transfection of mammalian cells using polyethylenimine (PEI) coated

gold nanoparticles

Graduate Teaching Assistant Sep’10 to May’11

Department of Chemistry, Louisiana Tech University

Duties included: Conducting 9 hours lab a week, Grading Exams and homework,

Preparing and presenting course material, Supervise 20 Undergraduate students

Trainee, Haffkine’s Research Institute, Mumbai, India Nov’06

Annual Biotechnology program

• Hands on experience on PCR, Agarose gel electrophoresis of DNA, SDS-PAGE

LAB SKILLS

Instruments

• Nanodrop Spectrophotometer, BTX Electroporator

• UV/VIS Spectrophotometer, Atomic absorption Spectroscopy, Infrared Spectroscopy

• Raman Spectroscopy, Gas Chromatography

• Fluorescence Microplate Reader, Autoclave, Centrifuge

Cell culture techniques

• Cell and tissue culture techniques including passaging and maintaining of mouse embryonic stem cells, 3T3 fibroblast, K562 cells and cryopreservation

• Isolation of fibrochondrocytes extracted from cow head

• Cell viability, freezing of cells, cell proliferation and culture based assays

• Viable cell count using hemocytometer

• Chemical and Physical transfection methods

Molecular Biology techniques

• PCR, Gel electrophoresis, SDS-PAGE

Microbiology

• Preparation of agar plates with antibiotics, media/buffer preparation, streaking agar plates with efficiency

Immunohistochemistry

• Staining, phase contrast and fluorescent microscopy, oct4 assay

Biochemistry techniques

• Alkaline Phosphatase Assay, MTT/MTS Assay

Imaging Software

• DP controller, Image J

RESEARCH PROJECTS

Tissue culture Laboratory, Louisiana Tech University

Thesis Project:

Transfection of mammalian cells using gold nanoparticles and gold nanowires coated with PEI

Tissue culture Laboratory, Louisiana Tech University

Project Title:

1) Sacrificial Microfiber bioreactors for 3D cell culture

2) Microfluidics mediated DNA delivery using electroporation

PUBLICATION (Manuscript-In preparation)

Enhance the transfection of mammalian cells using polyethylenimine (PEI) coated gold nanoparticles.

CONFERENCE PRESENTATION

Louisiana Tech University, Engineering and Science conference

“Enhance the transfection of mammalian cells using polyethyenimine (PEI) coated gold nanoparticles”.

TECHNICAL SKILLS

Operating System: MS Windows 98/2000/XP/Vista/7

Microsoft Office Suite: MS Word, MS Excel, MS Outlook, MS PowerPoint

REFERENCES

Dr. Shengnian Wang, Assistant Professor, Chemical Engineering/ IFM, Louisiana Tech University, Ruston, LA

Email: *****@******.*** 318-***-****

Dr. David Mills, Assistant professor, Biological Sciences/ IFM, Louisiana Tech University, Ruston, LA

Email: *******@******.*** 318-***-****

Dr. Long Que, Assistant professor, Electrical Engineering/ IFM Louisiana Tech University, Ruston, LA

Email: ****@******.*** 318-***-****

Danny Eddy, Instructor, Department of Chemistry, Louisiana Tech University, Ruston, LA

Email: *****@****.******.*** 318-***-****

Contact this candidate