Resume

Sanchit Trivedi *******.*******@*****.***

** ***** **** **., ******, MA 01854 978-***-****

OBJECTIVE

Molecular biology intern at Ion Torrent, Life technology looking for part – time position in molecular

biology related position.

SKILLS SUMMARY

• Strong analytical and technical skills, detail oriented and well organized

• Excellent verbal and written communication skills

• Ability to work in a team and independently.

• Cell Culture and molecular biology techniques, Next generation sequencing, Emulsion PCR, SDS-PAGE, Western-blotting,sterile microbiology techniques, PCR, cellular assays, ELISA, Chromatography, maintenance of adherent and non-adherent cell lines, Transfection in mammalian cell lines and transformation, passaging and staining of cells, flow cytometry.

ACADEMIC EXPERIENCE

• Cloning and expression of Paramecium Bursaria Chlorella Virus 1 (PBCV-1) gene.

Cloning Transformation of competent E.coli cells with a gene of interest from a virus genome using expression vector pMAL-c5E and then over express the protein encoded by the gene. The study focused on techniques like Southern blotting, Restriction mapping, Western Blotting, DNA sequencing reaction and analysis of different gels and Polymerization Chain Reaction (PCR).

• Effect of all trans-retinoic acid (ATRA) on Acute Myelocytic Leukemia (AML) cell line

This project was designed to determine the effect of different exposure time and concentration of all trans-retinoic acid on AML cell line. The treatment showed differentiation of the AML cells to mature blood cells.

• Isolation, Purification & determination of protein content and enzyme activity of wheat germ acid phosphatase.

Isolation and purification of the enzyme ‘wheat germ acid phosphatase’ from wheat germ lysate and then studying the kinetics of the enzyme using inhibitors. The protein content is measured in the course of purification process using Bradford Assay.

• Isolation of immunoglobulins from mouse.

During this study, techniques like isolation of cells from mouse spleen, ELISA to screen for immunoglobulin using different antigen, dialysis, salting out, screening of hybridomas using microELISA, Cytotoxicity Technique with Antibody and Complement, column chromatography, Bradford Assay, hemagglutination reaction, cardiac puncture and spectroscopy were practiced.

EDUCATION

Masters of Science in Biotechnology, University of Massachusetts, Lowell. (May 2013)

Relevant Coursework: Biochemistry, Molecular Biology, Cell Culture, Immunology (and lab)

Bachelor of Pharmacy, Nagpur University, India (July 2009) GPA – 3.79/4.00

Relevant Coursework: Pharmacology, Pharmaceutics,Microbiology, Pharmaceutical chemistry (and lab)

EMPLOYMENT HISTORY

Molecular Biology Intern, Ion torrent, Life technologies, Beverly, MA-01854 June 2012 – August 2012

• Working for next generation sequencing development projects for better genomic sequencing.

• Thorough experience with Personal genomic machine, Emulsion PCR, qPCR, Assays, data analysis.

Clinical Business Associate, Unichem Laboratories Ltd, Mumbai, India | Jan 2011- July 2011

• Researched clnical trials and shared scientific findings to enable Pharma clients in developing marketing strategies. Managed a team of four people.

Pharmacist, Emcure Pharmaceuticals Ltd, Pune, India | Sep 2009-Jully 2010

• Worked in research and development in compression department of Tablets and capsules.

• Hand on experience in the following techniques: Chromatography (HPLC).

Pharma Intern, Watson Pharmaceuticals Ltd, Goa, India. (Summer 2008)

• Documented procedures, processes for future references within company

COMPUTER SKILLS:

• MS Office (Excel, Word, PowerPoint), Windows Platform.

Contact this candidate