Lab Tech

PROFESSIONAL PROFILE

Laboratory professional with extensive experience in preparation of specimen and the operation of laboratory equipment. Solid foundation of experience working in a highly regulated, GMP environment along with aseptic procedures. Proficient with Beckman DU-7400 and Shimatzu spectrophotometers. Experienced with operating PCR machine and techniques involved with preparation of copying DNA and plasmids. Skilled in the operation of Biowave and ABEC cell culture reactors. Knowledgeable in operations of YSI Analyzer, Yokagawa pH converter, BGA Glucose analyzer, and Beckman Coulter ViCell Viability analyzer.

LAB EXPERIENCE & TECHNICAL SKILLS

Chemistry/Biochemistry

• Ascertained elements present in unknown mixtures

• Accessed and identified concentration of elements present in compounds

• Copied mitochondrial DNA from hair with the use of PCR and observed the results by means of agarose gel electrophoresis

• Used DNA enzyme to cleave RNA; a DNA/RNA hybrid was formed, stained, and run on a gel electrophoresis in order to study DNA kinetics

Biotechnology DNA/Cell Cultures

• Performed DNA isolation techniques, and extracted and analyzed DNA to facilitate the study of DNA enzyme behavior

• Maintained 3T3-Swiss Albino and RAW 264.7 animal cell lines by subculturing and studied their growth curve

• Trained to use different cell staining and aseptic techniques in addition to performing cytotoxicity tests

Genetics

• Altered E. coli DNA with antibiotic resistant plasmid and tested the acquisition of the trait on antibiotic plates

• Tested cut DNA with gel electrophoresis to determine acquired traits

• Gained exposure to cell transfections and various types of chromatography

WORK EXPERIENCE

April 2010 – Present Charles River Laboratories Malvern, PA

Cell Culture/Microbial Manufacturing Technician

• Grow and maintain multiple mammalian and microbial cell lines for testing

• Execute aseptic techniques, GMP, and GLP procedures in lab and clean room environments

• Knowledge of various cell lines maintained for multiple Fortune 500 pharmaceutical corporate clients

• Maintain detailed documentation of procedures in logbooks and batch records

• Formulate different types of media for cell lines to facilitate scale up and other fermentation activities

• Write and edit SOP and batch records

• Familiar with virological techniques and assays

December 2008 – September 2009 Kelly Scientific/Centocor Malvern, PA

Contractor/ Process Operator

• Performed manufacturing procedures and processes in a GMP environment for multi-billion dollar immunology product at a Johnson & Johnson subsidiary

• Knowledge of automated process control monitoring

• Conducted weighing and formulation of media for use in multiple downstream processes

• Perform CIP and SIP on vessels within a large biologic processing facility

• Analyzed and completed documentation for logbooks, batch records, and GMP documents

• Performed cleaning and sanitization of the production area in accordance to FDA and regulatory guidelines

• Proficient with different protein purification methods including HPLC, size exclusion chromatography and SDS-PAGE

June 2008 – September 2008 Merck & Co. WestPoint, PA

Biotechnician

• Monitored and performed large scale fermentation for vaccine operations in a GMP environment within the biotech manufacturing department

• Completed in-process samples for analysis of growth and conditions of cells throughout their matriculation in the fermentation process

• Assembled, disassembled, and calibrated fermentation tanks and other manufacturing equipment

• Performed extensive, detailed clean room and decontamination cleaning procedures

• Knowledge of data collection, data analysis, and data interpretation

• Trained in working on proprietary automated fermentation process system

• Knowledge of Batch and Continuous Process Control systems

August 2007 – March 2008 GlaxoSmithKline King Of Prussia, PA

Contractor/ Manufacturing Operator

• Performed large scale cell culture/ fermentation operations in a GMP pilot plant and GMP laboratories

• Responsible for preparation of reactors before production and maintenance in between production runs

• Acquired samples from reactors for analysis

• Prepared media and cell suspensions for inoculation into the reactors

• Familiar with monitoring automated process control systems

• Trained new employees as needed on equipment and procedures of the lab

• Proficient with different protein purification methods including HPLC, size exclusion chromatography and Ion exchange chromatography

2003 – 2008 All Seasons Retail Services NJ and PA Locations

Assembly Technician

• Provided contract based assembly services for home improvement retail outlets

• Managed crew of independent contractor assembly laborers

October 2006 – April 2007 Coriell Institute Camden, NJ

Cell Culture Lab Technician

• Administered aseptic techniques used to maintain clean cell lines within a non-profit laboratory organization

• Maintained comprehensive records and documentation of cell lines

• Performed duties within strict specification of a GLP regulated environment

Summer 2004 International Specialty Products Wayne, NJ

Lab Technician Intern

• Formulated skin and hair care products

• Maintained detailed records of experimental data

• Handled Viscometers and pH meters

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Summer 2002 William Paterson/Project Newark Wayne, NJ

Resident Teaching Assistant

• Supervised a group of 30 high school students in summer college-prep program

• Served as a tutor and mentor to program participants

2000 – 2005 William Paterson University Wayne, NJ

Security Manager

• Trained, supervised and scheduled a team of desk associates

• Conducted staff meetings and performed conflict resolution

APPLICATION SKILLS

Microsoft Office Suite including: Word, Excel, and Outlook

EDUCATION

May 2006 William Paterson University Wayne, NJ

B.S. in Biotechnology

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