Development Project

Wandong (Wendy) Zhang, Ph.D.

*xxx **th Avenue, San Francisco, CA 94122

********@*****.***;

936-***-**** mobile

EDUCATION

Ph. D., biochemistry and molecular biology; 1996, Univ. Medicine & Dentistry of NJ at Rutgers, NJ

B.S., biology; 1990, Tsinghua University, China

WORK EXPERIENCE

1/2010 – specialist, UCSF San Francisco, CA

1. iPS cell model of monogenic disorder: human primary fibroblast, human ES/iPS cell culture, stem cell differentiation, biological assays for phenotypes;

2. gene expression analysis based on eQTL, siRNA knockdown, QPCR.

4/2002- 1/2009 Scientist/Sr. Scientist, Neuroscience drug discovery, Lexicon Pharmaceuticals, Inc. TX

Hands-on biochemical, cell based and ex vivo assay development for HTS and SAR, and preclinical biomarker development, for over 6 small molecule drug programs and 1 antibody drug program.

1. Plasmid construct design, subcloning, stable cell line establishment; protein expression and purification.

2. Receptor/ligand/adhesion molecule binding, immunoprecipitation/western in ex vivo native tissue and recombinant cell lines.

3. cell based assays using alpha screen, GTPgS binding, and TR-FRET. Agonist/antagonist screen.

4. Antibody therapeutics functional assays; monoclonal antibody production, and phosphor-antibody reagent development ELISA, immunoassay.

5. Developing compound LX6171 to IND and clinical trial phase 2 for cognition. Medicinal chemistry support and lead discovery. Determining the pharmacological parameters in ex vivo native tissues for LX617 IND submission.

Managerial and organizational roles

1. Project scientist in cross-functional teams and with Bristol-Myers Squibb alliance. Organized and presented at working groups and teleconferences.

2. Supervised research associates; analyzed complex data and prepared formal reports. Other managerial roles such as FTE recording/tracking; annual budget planning for capital expenses; animal protocols; hazard safety.

1998-2002 Postdoc Fellow; Instructor; Mt. Sinai Medical School, New York, NY

1. Protein functions (cadherins, actin, Citron) in synapse development modeled by cultured primary neurons.

2. Establish, trouble shoot and ran live cell imaging system in the lab.

1996-1998 Postdoc Scholar, California Institute of Technology, Pasadena, CA

1. identification of novel signaling and scaffold proteins from rat postsynaptic density by protein purification, peptide sequencing and cloning.

2. antibody generation; protein functions by yeast two hybrid and binding assay.

1990-1996 Ph.D candidate, UMDNJ/Rutgers, Piscataway, NJ.

1. Cloning of a family of Ser/Thr kinase genes by Southern blots, phage library hybridization and DNA sequencing in bacterium M. Xanthus; Studied their expression and functions by gene deletion, and lacZ reporter.

2. Ser/Thr kinase biochemical characterization by protein expression, purification and kinase assay, phosphor-amino acid mapping.

TECHNICAL EXPERTISE

1. R&D skills pharmaceutical industry; excellent research and grant writing experience academia.

2. Protein biochemistry: purification, EIA/RIA, AKTA explorer, HPLC, activity assays. BIACORE for kinetics and interaction (small molecules and proteins). Protein purification and bioprocessing: intensive course by GE 2011

3. Expertise in ES cell biology, induced pluripotency cells, primary cell culture, FACS and quantitative imaging. Expert skills in molecular cloning, QPCR, DNA/RNA procedures. Extensive rodent handling.

SCIENTIFIC PUBLICATIONS

1. KK. Appiah, WA. Kostich, SW. Gerritz, Y. Huang, BD. Hamman, J. Allen, W. Zhang, TL. Lanthorn, R. Westphal, JC. O’Connell. A High-Throughput Screen for Receptor Protein Tyrosine Phosphatase-gamma Selective Inhibitors. The Journal of Biomolecular Screening 2011

2. W. Zhang, KV. Savelieva, A. Suwanichkul, D. Small, LL. Kirkpatrick, N. Xu, TH. Lanthorn, G-L Ye. Transmembrane and ubiquitin-line containing 1 (Tmub1) Regulates Locomotor Activity and Wakefulness in Mice and Interacts with CAMLG. PLoS ONE 2010 e11261.

3. I Rajan, K Savelieva, G-l Ye, C-y Wang, M Malbari, C Friddle, T Lanthorn, W Zhang*. Loss of the putative catalytic domain of HDAC4 leads to reduced thermal nociception and seizures while permitting normal bone development. PLoS ONE 2009 e6612

4. X Yu , W Zhang, A Oldham, E Buxton, S Patel, N Nghi, D Tran, TH Lanthorn, C Bomont, ZC Shi and Q Liu. Discovery and characterization of potent small molecule inhibitors of the high affinity proline transporter. Neuroscience Letters 2009, 451:212

5. GL Ye, KB Baker, SM Mason, W Zhang, L Kirkpatrick, TH Lanthorn, and KV Savelieva: GABAA receptor α1 subunit Gabra1 knockout mice: review and new results. In “Mutant and Transgenic Tools in Modeling Brain Disorders”, Edited by Allan V. Kalueff, Carisa L. Bergner, Humana Press, 2009.

6. W. Zhang, I. Rajan, K. V. Savelieva, C. Y. Wang, P. Vogel, M. Kelly, N. Xu, B. Hasson, W. Jarman & T. H. Lanthorn. Netrin-G2 and netrin-G2 ligand are both required for normal auditory responsiveness. Genes, Brain and Behavior 2008, 7, 385.

7. W. Zhang*, DL Benson. Targeting and clustering citron to synapses. Mol Cell Neurosci. 2006, 31:26-36.

8. Bekirov, L. Needleman, W. Zhang, D. Benson. Developmental regulation and circuit specific distribution of classic cadherins in the rat limbic system. Neuroscience 2002, 115:213-27

9. W. Zhang and D. Benson. Developmentally regulated changes in cellular compartmentation and synaptic distribution of actin in hippocampal neurons. J. Neuroscience Res. 2002, 69, 427-436

10. W. Zhang and D. Benson. Stages of synapse development by dependence on F-actin. J. Neuroscience 2001, 21, 5169-5181

11. G. Phillips, J. Huang, Y. Wang, H. Tanaka, L. Shapiro, W. Zhang, W. Shan, K. Arndt, M. Frank, R. Gordon, M. Gawinowicz, Y. Zhao, D. Colman. The presynaptic particle web: ultrastructure, composition and reconstitution of its components. Neuron 2001, 32, 63-77

12. W. Zhang and D. Benson Development and functional organization of dendritic spines. Hippocampus 2000, 10, 512-526 (Review)

13. W. Zhang, L. Vasquez, M. Apperson, and M. Kennedy. Citron, a PSD-95 binding protein at glutamatergic synapses on inhibitory neurons of hippocampus. J. Neuroscience 1999, 19, 96-108

14. W. Zhang, M. Inouye, and S. Inouye. Reciprocal regulation of the differentiation of Myxococcus xanthus by Pkn5 and Pkn6, eukaryotic-like protein Ser/Thr protein kinases. Molecular Microbiology 1996, 20, 435-447

15. W. Zhang, J. Munoz-Dorado, M. Inouye, and S. Inouye. Identification of a putative eukaryotic-like protein kinase family in the developmental bacterium Myxococcus xanthus. J. Bacteriology 1992, 174, 5450-5453

PATENTS

Listed as an inventor on pending U.S. Patents. Contact Lance Ishimoto for verification 281-***-****

Contact this candidate