SANG YOON LEE
***** ******* ****. *** **** WOODLAND HILLS, CA 91367
PHONE 971-***-**** • **********@*******.***
Objective
A position in Biotech/Pharmaceutical environment providing the opportunity to make a strong contribution to organizational goals through continued development of professional skills.
Education
University of North Carolina at Chapel Hill
• Bachelor of Arts, Chemistry (Dec. 2008)
• Cumulative GPA: 3.45
Work Experience
Amgen Inc., Thousand Oaks, C.A
Formulation and Analytical Resource, P&PD
Contingent Worker: March 2009- present
• Develop, analyze, and present data on several protein product formulations concerning aggregation and stability using size exclusion HPLC, anion exchange HPLC, reversed phase HPLC(non-reduced and reduced and alkylated), CE-SDS, and HIAC particle counter
• Analyzing how proteins act in different environments (temperature, time, and buffers) and study about protein behavior using different classes of excipients on visco-elastic properties of high concentration protein formulations by using HPLC, Micro Flow Imaging, Quartz Crystal Microbalance, and Advanced Rheometric Expansion System
• Helped the team in preparing for new studies (filling and vialing)
• Helped in compiling data in characterizing different proteins in assays such as ELISA, pH, osmolality and viscosity
University of North Carolina Biochemistry and Biophysics Lab, Chapel Hill, N.C
Laboratory Technician: May 2007- December 2008
• Assisted Dr. Julia E. Brittain working in the sickle cell field at Parise’s laboratory
• Did lab work on human plasma, blood, white cells, red cells, etc.
• Worked on the relationship between pulmonary hypertension in Sickle Cell Disease (SCD): Researched about PlGF, sVCAM, Nitric Oxide, Cystatin C, sFLT-1, VEGF in SCD.
•Separated plasmas from blood for each assays and compared SCD samples with control
• Micropipette, centrifuge, dilution, incubation skills were frequently used and Enzyme-Linked Immunosorbent Assay was performed
• Organized data and analyzing the results of the ELISA.
Korea Food and Drug Administration (K.F.D.A)Seoul, Korea
Office Assistant: January 2002- May 2004
Office Assistant
• Filing and Investigation food and drug labels
• Helped individuals in the process of importing food to Korea
• Learned basic lab skills in the laboratory
Skills/Qualifications
• Protein purification
• HIAC particle measurement
• Enzyme- Linked Immunosorbent assay (ELISA)
• Quartz Crystal Microbalance (QCM)
• High Performance Liquid Chromatography (HPLC) - Size Exclusion, Anion- Exchange, Reverse Phase
• Capillary Electrophoresis-Sodium Dodecyl Sulfate (CE-SDS)
• Advanced Rheometric Expansion System (ARES)
• Brookfield Rheometer
• Plasmid purification
• Buffer and media preparation
• Rotary evaporator
• Micro Flow Imaging (MFI)
• SDS-PAGE
• Osmolality
• Titration and Filtration
• pH
Computer Software
• Microsoft: Windows, Word, Excel, PowerPoint, Outlook
• Research: Data studio, Spin Works 2.2.5, Scifinder, SigmaPlot 11.0, ChemStation, Chromeleon, UV-Vis Chemstation
Relevant Course Work
• General Chemistry with Lab • Organic Chemistry with Lab
• Analytical Chemistry with Lab • General Physics with Lab
• Microbiology with Lab • General Biology with Lab
• Human Anatomy and Physiology with Lab • Statistics
• Cellular Biology with Lab • Genetics
• Biochemistry •Physical Chemistry
Extracurricular Activities
•12th annual Gainesville State College Math Tournament (2006)
With 3 other colleagues won 3rd place on the team round and 2nd place overall
• 3 abstracts are going to be published on Sickle Cell Disease research
References are available upon request