Transdisciplinary Big-Data Aging Health Research

Huilong Xie

Date of Birth: ****.** Address: Putian, Fujian, China

Email:*********@***.*** Tel:+86-199********

Educational Background

Guangzhou Sports University (Full-time; GPA: 3.71/4.00) Master’s degree

Sports Kinesiology 2023.09-2026.07

Ningde Normal University (Full-time; GPA: 2.84/4.00) Bachelor‘s degree

International Business 2021.09-2023.07

Fujian Sports Vocational Education and Technical College (Full-time; GPA: 2.85/4.00) Associate degree

Social Sports 2018.09-2021.07

Academic publications

First-author publications: 2 published, 1 accepted. Co-first-author manuscripts: 3 under revision or in peer review.

[1] Huilong Xie, Xuebing Sun, Liyu Huang, Tao Wang, Qi Yu, Min Hu*, Jingwen Liao*. Sex-specific associations between physical activity and cardiovascular disease under air pollution among the middle-aged and elderly population: a nationwide cross-sectional study in China. The journal of nutrition, health & aging. 2026 Jan 30(1), 100731. https://doi.org/10.1016/j.jnha.2025.100731 (CAS:Q2, JCR: Q2, IF: 4.0)

[2] Huilong Xie#, Bo Chen#, Fan Xia, Jingbo Xia*. The association between physical activity and cardiovascular disease under indoor air pollution among middle-aged-to-elderly people: a sex-specific analysis from CHARLS. BMC Public Health. 2026 Mar 30;26(1):1513. https://doi:10.1186/s12889-026-27175

-w (CAS:Q2, JCR: Q1, IF: 3.6)

[3] Huilong Xie#, Linjie Shu#, Ran Cheng, Min Hu*, Jingwen Liao*. Shift work and early vascular aging: a systematic review. BMC public health.

(CAS:Q2, JCR: Q1, IF: 3.6, accepted)

[4] Tianyu Sun#, Huilong Xie#, Junhao Huang*. Associations of socioeconomic status and physical activity on depressive symptoms among adults in China: Evidence from the China Family Panel Studies. BMC public health. (CAS:Q2, JCR: Q1, IF: 3.6, major revision)

[5] Guangyuan Sha#, Huilong Xie#, Liyu Huang, Yuhong Wang, Weiwei Huang, Jingbo Xia*. Physical activity, air pollution, and incident cardiometabolic multimorbidity in middle-aged and older Chinese adults: a longitudinal cohort study with mediation analysis. BMC Cardiovascular Disorders. (CAS:Q3, JCR: Q2, IF: 2.3, peer-review)

[6] Guangyuan Sha#, Huilong Xie#, Qibin Deng, Jinyun Wang, Weiwei Huang, Min Hu*, Jingbo Xia*. Sarcopenic obesity defined by different obesity indices and its cumulative exposure to all-cause mortality in elderly Chinese: evidence from the CHARLS. Aging Clinical and Experimental Research.

(CAS:Q3, JCR: Q2, IF: 4.7, peer-review)

[7] Ran Cheng#, Mengsi Yan#, Chaoge Wang, Linjie Shu, Zhengmao Peng, Jie Zhou, Huilong Xie, Junhao Huang, Min Hu*, Jingwen Liao*. Effects of Exercise on PVAT-Derived Exosomes in the Modulation of Vascular Function in Obese Mice. Appl Biochem Biotechnol. 2026 May 27. https://doi.org/10.1007/s12010-026-05743-5. (CAS:Q3, JCR: Q2, IF: 3.5) Project Experience

1. Provincial/Ministerial Project:

Role of PVATAdrenergic Signaling in the Exercise-Induced Regulation of Vascular Circadian Rhythms Fifth Participant 2025 – Present

• Collaborated with a team of 4 members to investigate the crosstalk between perivascular adipose tissue (PVAT) and vascular circadian clocks under exercise intervention.

• Coordinated daily lab routines, including animal welfare monitoring, treadmill maintenance, and reagent inventory tracking.

• Responsible for establishing and maintaining a rhythm disorder model; performed treadmill exercise training, tissue harvesting (PVAT, aorta), and routine histological processing.

• Conducted vascular ring myography to assess endothelium-dependent and endothelium-independent vasodilation/constriction of isolated aortic rings.

• Performed RNA-seq library preparation and preliminary bioinformatics analysis (differential expression, pathway enrichment) to identify circadian-related genes modulated by PVAT adrenergic signaling.

• Validated key targets using qPCR, Western blot, and immunofluorescence staining of paraffin-embedded arterial sections. 2. Bureau-Level (City/Prefecture) Project:

Epigenetic Regulation of Smooth Muscle Cell Phenotypic Switching by PVAT-Derived Exosomal miR-122-5p in Exercise-Alleviated Obese Vascular Remodeling

Third Participant 2024 – Present

• Worked closely with 4 lab members in executing the project, ensuring smooth communication between animal surgery, cell culture, and electrophysiology subgroups.

• Managed day-to-day laboratory operations (e.g., equipment booking, buffer preparation, safety compliance) and assisted in training junior students in basic molecular techniques.

• Isolated and characterized primary vascular smooth muscle cells (VSMCs) and PVAT-derived exosomes from mouse models using ultracentrifugation and nanoparticle tracking analysis.

• Performed cell culture experiments (VSMC phenotype switching assays, exosome co-incubation) to validate the functional role of exosomal miR-122-5p.

• Conducted miRNA sequencing and bioinformatics target prediction to identify downstream pathways.

• Verified candidate genes by RT-qPCR (miRNA and mRNA) and Western blot (VSMC marker proteins).

• Organized project-related documentation (animal ethics, reagent ordering, experimental records) . Academic conferences

[1] Conference: The 3rd Report Conference on Integration of Medicine and Sports of the World Federation of Chinese Medicine Societies

(WFCMS)

Location & Date: Zhejiang, China, 2025

Author contribution: First author

Title: Association analysis ofphysical activity andprevalence ofhypertension in middle-aged and older adults under exposure to ambient particulate matter PM10. (Oral presentation)

[2] Conference: The 14th National Congress of Sports Science Location & Date: Wuhan, China, 2025

Author contribution: Second author

Title: Association between physical activity, solid fuel use, and prevalence of hypertension in middle-aged and elderly adults.

(Oral presentation)

Academic skills

1. Data Analysis: Proficient in R-based statistical modeling (e.g., generalized linear models, mixed-effects models, survival analysis, mediation analysis) and cohort data harmonization (data cleaning, integration across multiple cohorts, internal/external validation). Additionally, skilled in “vibe coding” for statistics – leveragingAItools(e.g., ChatGPT, GitHub Copilot) together with systematic reading ofRpackagedocumentation/vignettes (e.g., tidyverse, survival, lme4, tableone, ggplot2) to rapidly generate, test, and refine analytical code.

2. Graphical Design: Generate publication ready statistical graphics (e.g., forest plots, volcano plots, correlationmatrices,dose response curves, and annotated ggplot2 figures), as well as design graphical abstracts and summary figures. 3. Experimental & Technical Skills:

- Human exercise intervention – Posture assessment, active/passive muscle stretching and release, pre activation for muscle training, resistance training instruction (certified Chinese Fitness Instructor, etc.).

- Anthropometric data collection – Participant recruitment, questionnaire design and administration, accelerometer operation.

- Basic molecular biology techniques – Animal handling and intervention, tissue sampling, PCR, Western blot, immunofluorescence, HE staining, cell culture.

4. English Proficiency:

- College English Test Band 6 (CET-6) certified; currently preparing for the IELTS examination.

- Experienced in topic selection, study design planning, and manuscript writing.

Contact this candidate