Senior Microfluidics Engineer and Instrumentation Lead

*

Daniel Qi, PhD.

San Lorenzo, CA *****

Tel: 510-***-****,

Email: *********@*****.***

www.linkedin.com/in/daniel-qi-004b262

SUMMARY:

Principal / Staff Engineer specializing in microfluidic systems, diagnostic cartridges, and life science instrumentation. 20+ years leading development from concept to commercialization across NGS, PCR, and immunoassay platforms. Expert in integrating fluidics, materials, chemistry, and hardware into manufacturable products.

SKILLS:

Technology: MEMS, Microfabrication, Microfluidics, Polymers and Elastomeric Materials, and Biochemistry

Engineering: SolidWorks, AutoCAD, CNC Programming and Machining, Molding Process, 3D Printing, Laser Engraving, Microscope Metrology, Verification Test Software: Windows, Office, JMP, LabVIEW, CorelDraw, Project Management PROFESSIONAL EXPERIENCE:

Sequlite, Pleasanton, CA May 2025 – Present

Sr. Engineer

Fluidic system development and process development for NGS instrumentation

SolidWorks CAD design of fixtures and devices

Alveo Technologies, Alameda, CA April 2022 – Nov 20204 Sr. Engineer

SolidWorks CAD design and development of a microfluidic plastic cartridge

SolidWorks thermal simulation for Anlyzer heater

Combination of chemicals to fluidic devices.

Adhesive process development

Force study of actuation of fluidic control

Metrology study of critical dimensions of injection molded parts

Verification Testing of the integrated cartridge

Packaging Design for Thermal Formed Parts

Acorn Product Development, Inc., Newark, CA Jan 2022 – April 2022 Sr. Engineer

Identify and develop critical interfaces for consumables with life science instruments.

Develop key subsystems including thermal control, liquid handling and other components of complex instruments.

2

Participate in complex system integration efforts and root cause identification efforts.

Perform hands-on assembly and testing of new systems

Work with life science clients to identify requirements; brainstorm, define and develop product solutions.

Guide Design Verification Testing and test efforts to confirm complex systems meet performance objectives.

Interface with and select vendors for prototyping, tooling & production. Bio-Rad Laboratories, Pleasanton, CA May 2021 – Dec 2021 Sr. System Engineer

Fluidic Manifold design review

Droplet Generation System Feasibility Test and Verification Test

Assembly Procedures of the Auto Droplet Generation System Fapon Biotech Corp, Fremont, CA Mar 2018 -- May 2021 Engineering manager

Project management of Immunoassay microfluidic cartridge development

Instrument design and integration for Immunoassay

Design Verification

Technical requirements management

Microfluidic Cartridge design and test

Surface chemistry study of polymeric materials for immunoassay

Verification Tests on the instrument including test plan, design of fixtures, data analysis, identification of technical findings.

Miroculus, San Francisco, CA Sep 2017 - Mar 2018

Mechanical Engineer

Fluidic Cartridge design with On-shape and development

Injection molding manufacturing process

Design Verification

Thermal Simulation for Microfluidic Droplets with SolidWorks

Plastic surface modification

Complete Genomics, Mountain View, CA

Staff Process Engineer Jun 2016 - Dec 2016

Flowcell process development

Evaluation of off-site manufacturing process

Optimize in-house process of adhesive depostion for flowcells

Metrology for QC process

SolidWorks Design Assembly fixture and program process instrumentation

Innovation in process technologies

Vivalnk, Santa Clara, CA Sep 2015 - Mar 2016

Consultant

3

Wearable medical device development

Manufacture process documentation

Maxim Integrated, San Jose, CA Apr 2015 - Aug 2015 Consultant

Microfluidic mobile device development

Lathrop Engineering, San Jose, CA Dec 2014 - Apr 2015 Sr. System Engineer

Microfluidic system development

Developed a microfluidic device for sample collection in a cell sorting flow cytometry system.

Developed a system for concentrating the sample.

Ansys simulation for fluidic system design.

DigiPath, Mountain View CA Jun 2014 - Dec 2014

Consultant

Development of a digital imaging system for Pathology.

System inventory management

IntegenX Inc. (formerly Microchip Biotechnologies), Pleasanton, CA Jan 2007- May 2013 Senior Scientist

Development of a microfluidic chip based on MOV technology for STR analysis

Surface modification of the microchip for STR

Design and Integration of a device for forensics sample prep

Design control with Solidworks PDM system

Fabrication technology on a microfluidic chip with polymeric materials

Thermal performance optimization for separation

Reagent seal technologies for integrated microfluidic cartridge

Combination of Chemical Reagents and fluidic devices

Design and fabrication technologies of elastomeric gaskets for microfluidic cartridge

Development of commercialized microfluidic cartridge with the RapidHIT system

Quality control process of injection molding and reagent seal process Theranos Inc., Menlo Park, CA 2005-2006

Senior Scientist

Development of a fluidic cartridge for an ELISA assay

Material compatibility study of plastics and adhesives

Fluidic cartridge design and test

Development of valves for flow control

Blood sample introduction to the fluidic cartridge

Fabrication of prototype fixtures

Polymer surface analysis and Quality Control process development

Coordination of development and oversea manufacture with ISO 13485 process 4

Clinical Micro Sensors, a Motorola Company, Pasadena, CA Jan 2001 - May 2005 Senior Staff Scientist

New generation of integrated cartridge that features rapid PCR and gene detection

Improved thermal control to increase PCR ramp rates of the integrated cartridge

Investigated biocompatibility of polymeric materials for an integrated microarray system

Developed approaches for fluidic mixing of PCR and Hybridization buffer on a cartridge

Enhanced detection limit and speed of multiplexed SNP analysis by mixing technologies

Designed the next generation of the commercialized integrated cartridge

Programmed CNC machining and designed mold for module assembling

Analyzed DNA hybridization data with Statistical tools.

FDA appliance documentation

Louisiana State University, Baton Rouge, Louisiana Sep 1997-Jan 2001 Postdoctoral Research Fellow

Microfabricated PMMA devices using lithography technology and an integrated microchip system with Near IR detection system for DNA analysis

Designed and built Nano level PCR thermal cycler system

Designed micro devices using AutoCAD

Generated optical masks, X-ray masks with Lithography methods

Fabricated PMMA microchips with X-ray Lithography

Developed thermal bonding process for PMMA microchips

Interfaced silica capillary to microchip for sample introduction

Modified silicon capillary inner surface to reduce EOF

Separated oligonucleotides using the Micro-CE system

Designed the interface between the microchip and the mass spectrometry

Integrated Near-IR detection system for micro-CE Brigham Young University, Provo, Utah Sep 1996 - Aug 1997 Postdoctoral Research Fellow

Modified inner surface of polymeric hollow fiber for capillary electrophoresis

Extruded plastic hollow fibers

University of Utah, Salt Lake City, Utah Sep 1995 – Aug 1996 Postdoctoral Research Fellow

Developed a PCR-HPLC-CZE fluid system to improve the reproducibility of capillary electrophoresis for PCR products

Investigated the absorption of protein on different polymer surfaces EDUCATION:

Ph. D. Analytical Chemistry, July 1995

Department of Chemistry, Peking University, Beijing 100871, China M. S. Analytical Chemistry, July 1991

Institute of Analysis and Measurement, the People's University, Beijing 100872, China 5

B. S. Chemistry, July 1988

Department of Technical Physics, Peking University, Beijing 100871, China LANGUAGES:

Proficient and fluent in English and Chinese

PATENT

• Point-of-care fluidic systems and uses thereof Issued: October 9, 2012, Patent number: 8283155, Inventors: Elizabeth A. Holmes, Shaunak Roy, John Howard, Chengwang Wang, Ian Gibbons, Timothy M. Kemp, Shize Daniel Qi

• Systems and methods for analyzing bodily fluids, Application number: 201********, Issued: March 1, 2012, Inventors: Elizabeth A. Holmes, Shaunak Roy, John Howard, Chengwang Wang, Ian Gibbons, Timothy M. Kemp, Shize Daniel Qi

• Systems and Methods of Sample Processing and Fluid Control in a Fluidic System, US 200******** A1, Sep 27, 2007 Inventors: Elizabeth A. Holmes, Shaunak Roy, Ian Gibbons, Shize Daniel Ql, Edmond Ku, Chris Todd, Melissa Takahashi, Bruce Johnson, Jeff Fenton, Keith Moravick, Uwe Springborn,Anthony Delacruz,

• Composite Plastic Articles, application number: 201********, Inventors: Boris Kobrin, Iuliu I. Blaga, William Nielsen, Shize D. Qi, Ezra Van Gelder PUBLICATION:

• Microfluidic devices fabricated in poly (methyl methacrylate) using hot-embossing with integrated sampling capillary and fiber optics for fluorescence detection. Shize Qi, Xuezhu Liu, Sean Ford, James Barrows, Gloria Thomas, Kevin Kelly, Andrew McCandless, Kun Lian, Jost Goettert, Steven A. Soper, Lab on a Chip (2002), 2(2), 88-95.

( See:

http://www.rsc.org/CFmuscat/intermediate_abstract.cfm?FURL=/ej/LC/2002/b200370h.PDF)

• Interfacing a Polymer-based Micromachined Device to a Nanoelectrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, Zhaojing Meng, Shize Qi, Steve Soper, and Patrick Limbach, Anal. Chem. (2001) 73 1286-1291

(See: http://pubs.acs.org/cgi-bin/abstract.cgi/ancham/2001/73/i06/abs/ac000984a.html)

• Microelectromechanical Systems (MEMS) Fabricated in Polymeric Materials: Applications in Chemistry and Life Sciences, Steven A. Soper, Sean Ford, Shize Qi, Robin L. McCarley, Kevin Kelly, Michael C. Murphy, Anal. Chem. (2000) 72, 643A- 651A

(See: http://pubs.acs.org/cgi-bin/redirect.cgi/ancham-a/0000/72/i19/html/soper.html)

• Nanoliter-Scale Sample Preparation Methods Directly Coupled to PMMA-Based Micro-Chips and Gel-Filled Capillaries for the Analysis of Oligonucleotides, Steven A. Soper, Sean M. Ford, Yichuan Xu, Shize Qi, Scott McWhorter, Suzzane Lassiter and, Don Patterson, J. Chromatogr. A 1999, 853:

(1-2) 107-120

(See: http://www.sciencedirect.com/science/journal/00219673 ) 6

• Micromaching in Plastics using X-Ray Lithography (LIGA) for the Fabrication of Micro- Electrophoresis Devices, Sean M. Ford, Jack Davis, Bill Kar, Shize Qi, Scott McWhorter, and Steven A. Soper, J. of BioMechanical Eng. 1999, 121, 1

(See: http://asme.org/pubs/journals/bioeng/toc_v121n1.html )

• Polypropylene Hollow Fibers Modified with PMMA for Capillary Electrophoresis, Shize Qi and Milton L. Lee, J. Microcol. Sep. 1998, 10, 589

• The Molecular shape effect in the separation of ionic isomers by capillary zone electrophoresis, Shize Qi, Xiaohong Fang, Aijin Huang, Yiliang Sun, Chinese Chem. Letter. 1996, 7(12), 1105

• Compensation of two kinds of bias in capillary zone electrophoresis quantitation and its application in internal normalization method, Shize Qi, Aijin Huang, Yiliang Sun, Anal. Chem. 1996, 68, 1342-6

(See: http://pubs.acs.org//cgi-bin/gap.cgi/ancham/1996/68/i08/pdf/ac950907t.pdf)

• Determination of Inosine 5’-Monophosphate and Guanosine 5’-Monophosphate in pig feed by capillary zone electrophoresis, Huwei Liu, Shize Qi, Yingnan Zhang, Aijin Huang, Yiliang Sun, J. HRC. 20(1997)242

• Analysis of synthetic colorant food additives by capillary zone electrophoresis, Huwei, Liu, Tao Zhu, Yingnan Zhang, Shize Qi, Aijin Huang, Yiliang Sun, J. Chromatogr. A 718(1995)448-453

(See: http://www.sciencedirect.com/science/journal/00219673 )

• Purity control of different bee venom milittin preparations by capillary zone electrophoresis, Shize Qi, Tao Zhu, Tong Zhao, Xiaohong Fang, Yiliang Sun, J. Chromatogr. B, 658(1994)397-403

(See: http://www.sciencedirect.com/science/journal/03784347 )

• Purity control of p-aminophenol during its electrochemical production and purification using capillary electrophoresis, Shize Qi, Aijing Huang, Yiliang Sun, Sepu (in Chinese, abstract in English), 1994, 12(5), 348

• Capillary gel electrophoresis (Review), Shize Qi, Tao Zhu, Yiliang Sun, Sepu (in Chinese, abstract in English), 1994, 12(2), 98

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