Biomedical Engineer - Cancer Genomics & NGS Expert

RAVI SHRIVASTAV

*****************@*****.*** 215-***-**** 50 W 34th St. New York, 10001

Professional Summary

I am a biomedical engineer and medical technologist with over 16 years of experience in cancer genomics, next generation sequencing, multiomics, clinical diagnostics, and biomedical research with in-depth knowledge in scientific experimentation, technical analysis, research operations, and project management.

Experience

Senior Research Tech, Integrated Genomics Operation, MSKCC, New York 2021- present

• Single-cell genomics and Next Gen Sequencing using 10X Genomics, Illumina, Oxford nanopore

• NGS library prep and quality assessment using different technologies, data reviews and report

• LIMS, data management, automation, protocol development, inventory management, billing, and orders

• Training and coordinating with investigators on experiment requirements and design Research Scientist, McDevitt Bioengineering Lab, New York University, New York 2018 – 2020

• Clinical test development and validation of biomarkers for oral cancer on lab-on-a-chip (SBIR-NIH project)

• Development of clinical decision tool for determining severity of disease in COVID-19 patients

• Immunoassay development for the detection of several drugs of abuse (US Army project)

• Engineering and optimization sensor microbeads to capture target analytes on microfluidic platform

• Microfabrication, testing and FMEA (Failure modes) of programmable bio-nano-chip (pBNC) devices Research Tech, Children’s Hospital of Philadelphia, Philadelphia 2017

• Conducted CAR T-cells for immunotherapeutic studies with Neuroblastoma cancer cells

• Designed and conducted experiments (DoE), presented to clinical researchers

• Performed plasmid cloning, DNA extraction, mRNA synthesis and transfection experiments

• Maintained cell cultures and conducted gene expression experiments on several cancer cell lines Research Assistant, Drexel University, Philadelphia 2016 –2017

• Developed cell identifying and sorting program using MATLAB software

• Developed protocols for collagen scaffolds testing, biomarker staining and imaging (Image J)

• Studied the effect of controlled DBD plasma on the CT26 cancer cells for oncological therapies

• Cultured and maintained colorectal CT26, HaCaT cancer cell lines and Mesenchymal Stem cells

• Microfabricated PDMS based Liver-on-a-chip devices, explored microfluidic biomedical applications Medical Laboratory Technologist, SRL Diagnostics, Kathmandu, Nepal 2012 – 2014

• Conducted human hematological, biochemical assays for diagnostics and therapeutic interventions

• Drafted QA-QC reports, calibration and validation of assays and analyzer performance

• Processed tissue specimens for histopathological analysis: processing, microtomy, staining

• Maintained inventory-audit for stock management, supervised technicians on LIS, specimen handling

• Troubleshot instrumental and analytical errors, ensured corrective and preventive actions (CAPA) Trainee, Bir Hospital Central, Kathmandu 2009 – 2011 Central Blood Transfusion Services, Nepal Red Cross Society 2010

• Analyzed clinical specimens for several routine and special pathological tests

• Performed component separation for leukocytes, plasma (FFP), platelets (PRP)

• Cultured and tested transfusion transmitted infectious agents in blood

• Conducted phlebotomy for blood donation, cross-matching, antibody screening and blood typing Publications

• Clinical Decision Support Tool and Rapid Point-of-Care Platform for Determining Disease Severity in Patients with COVID-19 2020

• Nanosecond pulsed Dielectric Barrier Discharge induced Anti-Tumor Effects Propagate Through the depth of Tissue via Intracellular Signaling 2017

Education

MS in Biomedical Sciences 2016

Dean’s Scholarship, School of Biomedical Engineering Drexel University, Philadelphia BSc in Medical Laboratory Technology 2012

Merit Full Tuition Scholarship Pokhara University, Nepal Professional skills

• Certified Project and Operations Management, LEAN/Agile methods, collaboration, team leadership

• Healthcare and regulatory guidelines: CFR, IRB, FDA, GCP, HIPPA, bioethical and biosafety standards

• Clinical research methods, laboratory techniques, MS Office suite, statistical and management programs

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