scientist, retail associate

TIMOTHY J. MILLER

484-***-**** ******.**.**@*****.*** https://www.linkedin.com/in/tim-j-miller

PROFESSIONAL SUMMARY

Knowledgeable biomedical research scientist with robust background in scientific research, contributing to several groundbreaking projects. Proven ability to utilize analytical skills and collaborative teamwork to drive research success. Adept at experimental design, data analysis, and technical writing. Consistently delivers impactful results through collaborative efforts and innovative problem-solving. SKILLS

• Protein expression, purification & analysis

• qPCR & ddPCR, ELISA, cell-based assays

• Cell culture (primary & established mammalian

cell lines), cell banking

• HPLC / FPLC using Cytiva systems

• Bacterial cloning, production & purification of

Adenovirus & AAV vectors

• Gene therapy

• Assay development and validation

• Proficient in Biovia ELN

WORK HISTORY

Associate Research Scientist, Drug Substance Development, 11/2022 - Present OCUGEN OPCO, INC. – Malvern, PA

• Supports the cell therapy manufacturing process, production and purification of recombinant proteins, and AAV expression in mammalian host systems.

• Supports the upstream process (USP) and downstream process (DSP) scale-up to support early and late- stage projects (gene therapy treatments for inherited retinal diseases).

• Utilizes purification techniques such as tangential flow filtration (with diafiltration & ultrafiltration).

• Process Development of the pipeline projects for pre-clinical and clinical applications.

• Currently focused on a project called NeoCart (a type of regenerative medicine / tissue engineering therapy for knee cartilage repair).

Protein Drug Development Formulation Scientist, 01/2017 - 10/2022 EUROFINS LANCASTER LABORATORIES, PSS – Malvern, PA

• Performed experimental studies related to formulation, process development and testing of therapeutic proteins (monoclonal antibodies), virus-like substances and gene therapy related molecules.

• Tests performed included MFI (micro-flow digital imaging) using Protein Simple MFI 5200 5000 Series, HIAC (High Accuracy Products; sub-visible liquid particle counting) using Beckman Hach HIAC 9703+, turbidity, pH, osmolality and color determination. Vector Production Manager, Department of Infectious Diseases, 02/2007 - 12/2016 CHILDREN'S HOSPITAL OF PHILADELPHIA RESEARCH INSTITUTE– Philadelphia, PA

• Produced and purified recombinant AAV viral vectors under GMP conditions by FPLC (using the ÄKTA Explorer Model 100A system) for use as experimental vaccines.

• Titered purified vectors using the ABI FAST Real-Time Quantitative PCR System.

• Cloned and purified vector plasmids used for calcium chloride transfection.

• Performed in vivo studies in rabbits to evaluate the safety and efficacy of vectors as potential vaccines that comply with FDA Investigational New Drug submission guidelines. Research Specialist / Department of Anesthesia, 02/2006 - 01/2007 UNIVERSITY OF PENNSYLVANIA PERELMAN SCHOOL OF MEDICINE – Philadelphia, PA

• Characterized the proteome of a novel glutamate receptor-associated transcription unit, GRINL-1A, by (1) antibody inhibition of NMDA cytotoxicity in cultured cortical and hippocampal neurons, (2) Western blots and (3) immunofluorescent staining (http://www.ncbi.nlm.nih.gov/pubmed/15233991).

• Used bacterial cloning to express and purify a peptide containing the putative anesthetic binding site of the nicotinic acetylcholine receptor alpha M2 subunit. Research Specialist / Department of Neurology, Neuro-oncology Division, 01/2005 - 01/2006 UNIVERSITY OF PENNSYLVANIA PERELMAN SCHOOL OF MEDICINE– Philadelphia, PA

• Performed immunoblots of patient sera and cerebrospinal fluid against proteins from cultured neuronal cells that express antigens associated with paraneoplastic neurologic syndromes (PNS).

• Screened cerebellar and brainstem cDNA libraries for PNS-associated antigens using phage display. Research Specialist / Institute for Human Gene Therapy, 01/2002 - 01/2005 UNIVERSITY OF PENNSYLVANIA PERELMAN SCHOOL OF MEDICINE– Philadelphia, PA

• Produced and purified recombinant adenovirus and AAV vectors in compliance with GLP/GMP standards.

• Cloned plasmids for production of viral vectors by transfection of mammalian cell lines.

• Analyzed transgene expression by Western / Southern blot, ELISA, FACS and PCR.

• Performed immunological assays to detect pre-existing neutralizing antibodies to AAV vectors. EDUCATION

B.A., Biology, University of Pennsylvania, Philadelphia, PA

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