Product Development Analytical Chemist Leader

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JOHN F. CLOS

Atlanta, GA 404-***-**** *********@*****.*** https://www.linkedin.com/in/john-clos/ ANALYTICAL CHEMIST DATA SCIENTIST

Highly experienced analytical chemist with expertise in research, development, operations support, supervisory processes, project management, and analytical support. Talent in providing consultation on analytical chemistry, and other technical areas, consistently succeeding in guiding cross-functional teams. Proven record of outsourcing general inquiries and stability studies to external laboratories as well as consulting on competitive product analysis, formula validation, method development, and research support. Driven by a sense of personal accountability, committed to creating a culture of excellence, quality, service, and profitability. For GRAS no objection letter for the use of rebiana (rebaudioside A, a natural high-intensity sweetener) in beverage, managed both the internal and external analytical projects to complete the necessary studies. Among the activities that was responsible for:

Methods development for the analysis of impurities and degradants in the ingredient as well as in solutions containing rebiana by HPLC-UV-CAD.

Consultation and collaboration with external laboratories for all analytical studies performed for obtaining GRAS approval of rebiana, including isolation, purification and identification of impurities and degradants; impurity profiling; powder and solution stability studies; and clinical studies. KEY TECHNICAL SKILLS

High-Performance Liquid Chromatography (HPLC) Liquid Chromatography/Mass Spectrometry (LC/MS) Ion-Exchange Chromatography (IC) Ion-Exchange Chromatography/Mass Spectrometry (IC/MS) Wet Chemical Methods Solid-Phase Extraction (SPE) and Chromatography Data Systems Data Analytics CAREER HIGHLIGHTS

Chemistry Content Developer – Macmillan Learning

• Transfer questions from textbook to their software, as well as crafting insightful hints, feedback, and solutions to address common errors and misconceptions. Principal Scientist – The Coca-Cola Company

• Created a python script to use an API to access PubChem to find compounds matching exact masses and their corresponding publications.

• Created a python script to build vitamin degradation models using stability study data and kinetic equations.

• Explored lambda function on AWS to automatically extract and database data and metadata in certificates of analysis (COAs).

• Investigated the implementation of Sample Manager LIMS and ELN in the R&D laboratories.

• Participated in internal and external committees to set specifications and review methods.

• Assisted in collecting R&D analytical methods from various authors around the globe and grouped the LC and IC methods for review refining this list to provide a set of preferred methods to reduce costs and improve data comparison between labs.

• Recruited various LC and IC subject matter experts within the Company to serve as associate coordinators and guided them through the process.

• Assisted in finding a lab (KRÜSS) to perform foam analysis for a project comparing foam stability between different milks added to espresso. Together with the KRÜSS team, we successfully conducted John F. Clos, Ph.D. 404-***-**** Page 2

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comprehensive experiments and analysis, contributing valuable insights to the project's objectives.

• Developed methods and performed analyses of problem samples to identify the contaminants by LC-QTOF to solve critical incidents.

• Performed a migration study of an additive in PET bottles various for FDA approval by LC-MS-MS.

• Evaluated a colorimeter program for automated colorant formulation.

• Resolved a %total titratable acidity error in a commercialized product using titration and IC. Scientist – The Coca-Cola Company

• Oversaw the development of a sensitive method for vitamin B12 in beverages by SPE and HPLC-UV.

• Provided analytical chemistry expertise for the Global Carbon Dioxide Supply Strategy Initiative, which was an internal initiative to recommend monitoring and purification equipment.

• Participated on the International Society of Beverage Technologists subcommittee on carbon dioxide.

• Developed a method for the analysis of dextran in beet sugar by NMR.

• Developed a simple method for the analysis of sucrose in beverage concentrate by TLC.

• Validated and assisted Corporate Quality Assurance in implementing a method for haloacetic acids analysis in water by GC-ECD.

• Performed a stability study of water-soluble vitamins in a model beverage with respect to relevant parameters.

EDUCATION

The University of Florida – Gainesville, FL

Doctor of Philosophy in Analytical Chemistry. Dissertation: A Self-Cleaning, Low-Temperature Molecular Luminescence Spectrometer as a Detector for Gas Chromatography. Advisor: Dr. James D. Winefordner, Ph. D. The University of Florida – Gainesville, FL

Master of Science in Analytical Chemistry. Thesis: The Effects of Surfactants on Adsorption at a Glassy Carbon Electrode in a Flowing Stream. Advisor: Dr. John G. Dorsey, Ph. D. University of Alabama at Birmingham – Birmingham, AL Bachelor of Science in Chemistry

PERSONAL DEVELOPMENT/CERTIFICATIONS

Project Management Institute

Certified Associate in Project Management

Georgia Institute of Technology – Atlanta, GA

Georgia Tech Data Science and Analytics Boot Camp

ASSOCIATION/AFFILIATIONS

Member of the American Chemical Society

Member on the 2015-2020 USP Food Ingredients Expert Committee including Vice Chair (2019-2020) Member of the AOAC Color Community

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PATENTS AND PUBLICATIONS

Stevia-derived molecules, methods of obtaining such molecules, and uses of the same US 12071452 B2 · Issued Aug 27,2024, WO 201-***-**** A1 20190523 · Issued Jan 1, 2019, WO 201-***-**** A1 20190523 · Issued Jan 1, 2019 Inventors: Purkayastha, S.; Markosyan, A.; Chow, S. Y.; Prakash, I.; Clos, J.; Peng, I. X.; Kagan, M. Z.; Sukits, S. F.; Somayajula, K. V.; Nizam bin Navi, K.

Rebaudioside A derivative products and methods for making WO 200-***-****, A2 20090903, WO 2009-US35108, WO2009108680 A3 · Issued Jan 1, 2009WO 200-***-****, A2 20090903, WO 2009-US35108, WO2009108680 A3 · Issued Jan 1, 2009 Inventors: Prakash, I.; DuBois, G. E.; San Miguel, R. I.; Clos, J. Prakash, I.; Clos, J. F.; Chaturvedula, V. S. P. Stability of rebaudioside A under acidic conditions and its degradation products. Food Research International (2012), 48(1), 65-75 Chaturvedula, V. S. P.; Clos, J. F.; Prakash, I. Fluorescent light exposure of rebaudioside A in mock beverages under International Conference on Harmonization (ICH) guidelines. International Journal of Chemistry (Toronto, ON, Canada) (2012), 4(3), 1-9

Prakash, I.; Clos, J. F.; Chaturvedula, V. S. P. Stability of steviol glycosides in mock beverages under acidic conditions. International Journal of Pharmacy and Pharmaceutical Sciences (2011), 3(Suppl. 5), 421-425 Chaturvedula, V. S. P.; Clos, J. F.; Rhea, J.; Milanowski, D.; Mocek, U.; DuBois, G. E.; Prakash, I. Minor diterpenoid glycosides from the leaves of Stevia rebaudiana. Phytochemistry Letters (2011), 4(3), 209-212 Chaturvedula, V. S. P.; Clos, J. F.; Prakash, I. Stability study of steviol glycosides in mock beverages using fluorescent light exposure under ICH guidelines. International Journal of Pharmacy and Pharmaceutical Sciences

(2011), 3(3), 316-323

Upreti, M.; Clos, J. F.; Somayajula, K. V.; Milanowski, D. J.; Mocek, U.; DuBois, G. E.; Prakash, I. Gas-phase intramolecular elimination reaction studies of steviol glycosides in positive electrospray and tandem mass spectrometry. European Journal of Mass Spectrometry (2009), 15(1), 11-21 Clos, J. F.; DuBois, G. E.; Prakash, I. Photostability of rebaudioside A and stevioside in beverages. Journal of Agricultural and Food Chemistry (2008), 56(18), 8507-8513 Prakash, I.; DuBois, G. E.; Clos, J. F.; Wilkens, K. L.; Fosdick, L. E. Development of rebiana, a natural, non-caloric sweetener. Food and Chemical Toxicology (2008), 46(7S), S75-S82 Clos, J. F.; Dorsey, J. G. Enhanced stability of electrochemical detection with surfactant containing mobile phases in liquid chromatography and flow injection analysis. Analytical Letters (1990), 23(12), 2327-37 Sentell, K. B.; Clos, J. F.; Dorsey, J. G. A practical approach to direct injection of untreated physiological fluids with micellar liquid chromatography: determination of bumetanide. BioChromatography (1989), 4(1), 35-40 John F. Clos, Ph.D. 404-***-**** Page 2

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VOLUNTEER WORK

Volunteer – ABF Foundation

• Identify National Landmarks along with a contact number that have the ability to light up yellow during Childhood Cancer Awareness month.

Volunteer – Kids First Initiative

• Conducted online research to find email contacts for 15-20 education, STEAM, news, or comic publications for their press release to unveil “STEAM Man” — A New Superhero to Enhance STEAM Education for Underserved Youth.

Volunteer – Everybody Solar

• Conducted online research to create a list of nonprofit organizations, facilitating Everybody Solar's mission to provide solar power to local charities, reducing electricity costs, and directing resources to community service.

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