Quality Control Laboratory Scientist

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AAYSHA DAWOOD

Tucker, GA, ***** 678-***-**** ********@****.*** LinkedIn

Molecular Laboratory Scientist Genomics & NGS Specialist Bioinformatics Analyst Detail-oriented Clinical and Molecular Laboratory Scientist with extensive hands-on experience in performing complex laboratory testing, operating advanced instrumentation, and executing molecular assays in clinical genomics.

• Proficient in DNA/RNA extraction, real-time PCR, next-generation sequencing (NGS), library preparation, and fragment analysis using platforms such as Illumina MiSeq, Hamilton STAR, ABI3500, Rotor-Gene Q, and QIAxcel.

• Demonstrated expertise in running diagnostic tests for hematologic malignancies and transplant monitoring, including chimerism studies and variant interpretation.

• Strong command of bioinformatics tools for variant analysis, quality control, and data reporting.

• Recognized for optimizing workflows, resolving technical challenges at the bench, and maintaining regulatory compliance (CAP, CLIA, HIPAA).

• Proven leader with experience mentoring staff, improving lab efficiency, and ensuring consistent delivery of accurate and reproducible results.

AREAS OF EXPERTISE

Molecular Diagnostics Next-Generation Sequencing (NGS) Bioinformatics Analysis Genomic Variant Interpretation Chimerism Analysis DNA/RNA Extraction Quality Control & Assurance Laboratory Automation Inventory Management Regulatory Compliance (CLIA/OSHA/CAP/HIPAA) Team Leadership & Training Protocol Development & Optimization Troubleshooting & Technical Support Real-Time PCR & Fragment Analysis Bone Marrow Transplant Monitoring PROFESSIONAL DEVELOPMENT

Technologist in Molecular Biology, MB (ASCP)CM American Society for Clinical Pathology (2019 – Present) PROFESSIONAL EXPERIENCE

Northside Hospital Atlanta, GA 10/2022 – 09/2024 Team Lead/Advanced Clinical Lab Scientist

Analyzed genomic variants relevant to patient diagnoses, particularly in cancer genomics, including somatic mutations and structural variants associated with hematologic malignancies. Ensured data integrity by implementing quality control measures, including preprocessing, filtering, and assessment of genomic data for accuracy and reliability. Utilized bioinformatics pipelines for NGS data analysis, including variant calling, annotation, and downstream analysis. Monitored chimerism engraftment in bone marrow transplant patients to detect early signs of rejection or relapse. Key Contributions:

• Led and motivated team members, delegating tasks effectively and fostering a collaborative work environment to achieve organizational goals.

• Optimized laboratory protocols to enhance efficiency, accuracy, and reproducibility of experimental procedures.

• Diagnosed and resolved technical issues in experimental workflows, minimizing downtime and ensuring seamless laboratory operations.

• Maintained comprehensive documentation, ensuring compliance with regulatory standards, including CAP, and trained laboratory personnel.

• Developed, reviewed, and updated SOPs to align with CAP standards, ensuring consistency and precision in laboratory operations.

Aaysha Dawood

Northside Hospital Atlanta, GA 08/2020 – 10/2022 Clinical Lab Scientist

Extracted high-quality DNA and RNA from diverse sample types, ensuring integrity and purity for downstream applications. Executed next-generation sequencing (NGS) procedures, including library preparation, sequencing, data analysis, and interpretation of genetic mutations in bone marrow and peripheral blood specimens. Operated Hamilton Star automated liquid handling systems for precise sample pipetting, reaction setup, and protocol optimization in NGS workflows. Assessed DNA library quality using the QIAxcel Advanced System for high-resolution capillary electrophoresis, including fragment analysis and sizing. Quantified DNA libraries with qPCR for accurate sample preparation and sequencing. Loaded and operated Illumina MiSeq sequencers, ensuring proper instrument setup, quality control, and troubleshooting for optimal performance. Conducted fragment analysis and genotyping using GeneMapper software (ABI3500) to support bone marrow transplant monitoring. Performed Chimerism studies to assess donor and recipient cell populations post- transplantation using Promega platforms. Operated Rotor-Gene Q and ABI3500 Genetic Analyzer for real-time PCR, fragment analysis, and conventional PCR applications, including gene amplification and mutation detection. Key Contributions:

• Implemented quality control measures across molecular laboratory procedures to ensure reproducibility, accuracy, and reliability of results.

• Diagnosed and resolved technical issues in NGS workflows, leveraging critical thinking and problem-solving skills to maintain lab efficiency.

• Maintained and troubleshot NGS platforms, such as Illumina, to prevent and resolve hardware and software errors.

• Ensured compliance with CAP quality control and assurance standards, including proficiency testing, equipment calibration, and validation processes.

Alpha Genomix Laboratories Norcross, GA 04/2018 – 05/2020 Laboratory Assistant

Conducted DNA extraction using various techniques to ensure high-quality nucleic acids for downstream applications. Operated QuantStudio systems for real-time PCR, including system setup, assay configuration, and monitoring runs for multiple assays, including copy number variation. Utilized the AccuFill system for precise and automated loading of OpenArray plates, ensuring accurate and consistent results. Performed Next Generation Sequencing (NGS) and MLPA

(Multiplex Ligation-dependent Probe Amplification) for oncology samples, adhering to Illumina protocols. Maintained integrity of patient samples during storage and processing stages. Key Contributions:

• Adhered to sterile techniques and routinely conducted lab sterilization procedures.

• Complied with CLIA, OSHA, CAP, and HIPAA regulations to maintain a safe and regulated laboratory environment.

• Maintained optimal inventory levels by proactively managing reagent and consumable stock, ensuring seamless laboratory operations through timely procurement and replenishment. EDUCATION

Bachelor of Science in Cell Biology and Biotechnology Georgia Gwinnett College, Lawrenceville, GA Associate of Science in Biology Georgia State University, Atlanta, GA LAB EQUIPMENT & SOFTWARE

Illumina MiSeq Hamilton Star QIAxcel Advanced System ABI3500 Genetic Analyzer GeneMapper software QuantStudio Systems Rotor-Gene Q AccuFill System Promega Platforms NGS-related bioinformatics tools

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