Molecular Biology Ph D

Sambavi Elangovan Ph.D

Molecular Biology Endocrinology In Vivo Modeling

** ********** **

Edison, NJ 08820

Email:**********@*****.***

Phone: 843-***-****

PROFESSIONAL SUMMARY

Highly dedicated and knowledgable molecular biologist with a focus on endocrinology. Adept and thorough researcher with a meticulous and rigorous approach to experiment design and analysis and animal handling. Ability to articulate endocrinal and biochemical concepts to both lay and professional audiences in a clear and accessible manner KEY COMPETENCIES

• Molecular biology techniques: Tissue culture, RNA & DNA isolation, PCR (qPCR, MS- PCR), ChIP assay, Gel electrophoresis (SDS-PAGE, Agarose), gel imaging and documentation (Bio-Rad), Western blotting, and Immunohistochemistry

• Surgical techniques: Ovariectomy, CSF collection, thyroidectomy, adrenalectomy and bilateral castration in Rat

• Biochemical techniques: Protein estimation, Thin layer chromatography, Paper chromatography, Ion- exchange chromatography, Radioimmuno assay (RIA), Immunoradiometric assay (IRMA), and Radioreceptor assay (RRA)

• Instrumentation expertise: Experience in handling laboratory instruments including Centrifuges, Ultracentrifuge, Spectrophotometer, Electrophoresis unit, pH meter, Microscopy(AFM), Gel Doc, and ChemiDoc XRS

WORK EXPERIENCE

• Medical University of South Carolina, Postdoctoral Scholar (PI: Dr. Jeremy Yu), Department of Medicine, Division of Endocrinology, Charleston, SC (Apr – July, 2021), Comparative analysis of human trophoblast cell lines - Modelling Preeclampsia by ELISA and RT-PCR (I have maintained BeWo, Jar, Jeg-3, and HTR-8/ SVneo cell lines) and I used to harvest the cells with 90% confluency and create hypoxic conditions and studied sFlt-1 and sEng levels and expressions and trained on RUPP model, CSF collection and Catheterization).

• Sampled, Piscataway, New Jersey, (Oct 2021- Jan 2023), Researcher in RT-PCR department, trained on liquid handler automations like hamilton, Sciclone, Zephyr, Chemagic 360. Extract and analysis of saliva, serum human samples and well established with RUCDR database LIMS (software used to productivity track of data associated with samples, experiments, laboratory workflows, and instruments), bioportal, FQC. I have efficiently participated in Audit Prep for Newyork state audit, CAP and Client audits. I am also experienced in PT tests and filling. Proficient in interpreting and presenting test results.

• QDx Pathology Lab, Edison, NJ, Molecular Technologist (2023-Mar 2025), I have trained on Flim Array, Panther, BioCode® Respiratory Pathogen Panel, BioCode® Gastrointestinal Pathogen Panel, parasitology, Dsx.

• Rutgers University, Researcher (PI: Dr. Christy Gliniak), Department of Nutritional Sciences, New Brunswick, NJ (Apr 2025 – Present). We are studying different types and their characteristics of adipose tissue in a transgenic mouse model. Maintaining 30 different strains/transgenic mouse colonies and 200 cages. I trained in genotyping, whole mount, flow cytometry, and SVF isolation. Along with that, I also trained in ordering chemicals in the RU marketplace, setting up the cell lab, and fixing the CO2 incubators. EDUCATION

• University of Madras at Madras, Ph.D. in Endocrinology (PI: Prof. M. Michael Aruldhas), Dr. ALMPG Institute of Basic Medical Sciences, Taramani, Tamilnadu, India. (2018)

• Ph.D.Dissertation title: In Utero and lactational exposure to di-(2- ethylhexyl)phthalate(DEHP) modifies the expression of genes involved in iodothyronine biosynthesis in puberal rats

• University of Madras at Madras, M.Sc. Biomedical Sciences, Dr. ALMPG Institute of Basic Medical Sciences, Taramani, Tamilnadu, India. (2009)

• M.Sc.Thesis title: Impact of transient neonatal hypothyroidism on cytochrome P450 aromatase mRNA expression in Sertoli cells of adult rats

• Thiruvalluvar University, B.Sc.Biochemistry (Adhiparasakthi Arts and Science college), Mullvadi, Vellore, India. (2007)

INTERNSHIP

• National Institute of Ocean Technology, Internship program (6 months training) (Ministry of Earth Sciences, Government of India), Pallikaranai, India. (2008)

• Internship title: Characterization of marine protease from Bacillus species. CONFERENCE PRESENTATIONS

• Elangovan, Sambavi; et al. “Endocrine Disruptors’’, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai (March 2014)

• Elangovan, Sambavi; et al. “International conference on recent trends in Biosciences and 32ndmeeting of the Society for Reproductive Biology and Comparative Endocrinology”, Holy cross college, Tiruchirappalli (January 2014)

• Elangovan, Sambavi; et al. “International Science Congress Association”, Karunya University, Coimbatore (December 2013)

• Elangovan, Sambavi; et al. “International conference on Frontiers in Biomedical sciences”, Holy cross college, Tiruchirappalli (August 2009)

PUBLICATIONS

• Sambavi Elangovan, Mariajoseph Michael Aruldhas, Suganya Sekaran, Rajesh Parsanathan, Suthagar Essaky, Ajit Kumar Navin, Shobana Navaneethabalakrishnan, Ravi Sankar Bhaskaran and Ilangovan Ramachandran. Gestational exposure to di(2-ethylhexyl) phthalate

(DEHP) modifies the expression pattern of genes controlling thyroid hormone biosynthesis in puberal rat progeny. Journal of Endocrinology and Reproduction 20:2(2016)

• Sekaran S, Balaganapathy P, Parsanathan R, Elangovan S, Gunashekar J, Bhat FA, Jagadeesan A. Lactational exposure of phthalate causes long-termdisruption in testicular architecture byaltering tight junctional and apoptotic protein expression in Sertoli cells of first filial generation pubertal Wistar rats. Human and Experimental Toxicology, 34:575-590 (2015)

• Venkatesh NS, Kathiresh Kumar M, Sambavi E, Kalpana N, Sashi John AM, Anbalagan J, Ilangovan R and Aruldhas MM. Transient neonatal-onset hypothyroidism boosts estradiol synthesis in the testis of adult Wistar rat. Journal of Endocrinology and Reproduction, 18:2455 – 1244 (2014)

PROFESSIONAL AFFILIATION

• Life member– Society for Reproductive Biology and Comparative Endocrinology (SRBCE), Chennai, India.

NOTE

• Authorized to work for any employer in the United States.

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