Biotechnological Researcher with Molecular Biology Expertise

Karishma Thakkar Anand, Gujarat, India +91-878******* E.mail: ******************@*****.***

Linkedin Profile: www.linkedin.com/in/karishma-thakkar-3320961ab Passionate researcher with a good background in biotechnological research and eager to learn about cutting- edge technologies for new innovation and development. Molecular Biology:

Molecular cloning for expression and over expression of protein.

Primer designing, PCR, Ligation, Transformation, Isolation and purification of DNA

Transformation in eukaryotic and prokaryotic cells, Clone confirmation

Cassette designing of genes for over expression of recombinant protein. Protein Expression & Purification (Soluble & Insoluble Form of Protein):

Protein expression in eukaryotic and prokaryotic cells

Protein purification and its qualitative and quantitative analysis

Clone confirmation, Formulation

Enzyme (Protease) activity and stability assessment

Literature survey, experiment designing and planning and trouble shooting. Other: Lipid producing strain isolation from soil sample and quantitative analysis

Plant Tissue Culture (Mycorrhiza production)

Plant protein extraction and purification

Bio-instrumentation and techniques: Microscope, UV-visible spectrometer, Nano-Drop, Western blot, SDS PAGE, Agarose Gel-electrophoresis, Thermocycler (PCR), Sonicator, centrifuge, Column Chromatography (Ion-exchange, Affinity, HPLC, Size exclusion), Cut Off Membrane, X-Ray Diffractometer (XRD), Dialysis.

Bioinformatic Tool: Primer 3, NEB Cutter, NCBI BLAST (Sequence analysis), Uniport, SMS, EMBL, Ensembl, Tm calculator, Oligoanalyzer, Expasy, EMBOSS Documentation: Experiment Report, SOP, Log book, COA, Product Data-sheet, BMR, BPR Preparation, Issuance and retrieval. In processes quality assurance (IPQA), GDP. PROFILE SUMMARY

Microbiology(Microbiological Assays): Sterilization, Sterility testing, Antimicrobial assays, Microbial Identification, Cultivation, maintenance and preservation of microbial culture.

Nanoparticle Synthesis, Characterization and its various application as an antimicrobial component. Antimicrobial activity determination (MLC, MIC) against resistant strains.

Lab maintenance, GLP experience

Research Associate Novumgen Pvt. Ltd. (Novumgen Biologics) Savali STBI, Vadodara, Gujarat March, 2025 to July 2025

Literature survey for therapeutic recombinant proteins

Therapeutic recombinant proteins development

Design of experiment

Research Associate Genexis Biotech Pvt. Ltd. Vadodara, Gujarat Oct, 2022 - Oct, 2024

Market research, literature survey for recombinant proteins and other molecules

Involved in experiment designing, planning, performing & trouble shooting

Molecular cloning, expression and over expression of recombinant protein (Cell culture media proteins, food proteins, cosmetic proteins)

Clone confirmation and Formulation of the protein and protease.

Primer designing, Restriction digestion

Designed a cassette of genes for over expression of recombinant protein

PCR, Ligation, Transformation, Isolation and purification of DNA

Eukaryotic and prokaryotic protein expression and purification

Process optimization (Molecular, Expression and Purification level including formulation and stability)

Basic (Lab Scale) qualitative and quantitative analysis of DNA and Protein Other Role and Responsibilities

Animal origin free cell culture media development.

Isolation of lipid producing strains from soil sample, lipid production and its quantitative analysis.

Isolation and purification of plant protein from plant extract.

Plant Tissue Culture (Mycorrhiza production)

Documentation (Product data-sheet, MSDS, SOP, Research proposal, Report)

Estimation of antimicrobial activity of biological compound

Worked on other external projects

Visiting Faculty L.P. Patel and CAM Institute of Allied Health Sciences Anand, Gujarat April’2022-October’2022

To Take lecture, viva and design question paper of theoretical and practical subjects. WORK EXPERIENCE

Quality Assurance Officer OSAKA Pharmaceuticals Vadodara December’2021-March’2022

To prepare document and issue like (BMR, BPR, SOP, COA)

In process quality assurance (IPQA)

Visiting Faculty ITM University Vadodara April’2021-July’2021

To Take lecture, viva and design question paper of theoretical and practical subjects. Intern Zydus Hospital Anand June 2019 - July 2019

Learned medical microbiology and microbial analysis of clinical samples

Recombinant Protein Development (Molecular biology)

Recombiant Protein Expression and Purification

Protein Expression Process Optimization

Protein Extraction and Purification

Experiment Designing and Trouble Shooting

M.Sc. MLT with Bio-pharma (From CHRUSAT) Anand, Gujarat

During the course with medical lab technology I also gained knowledge of bio-pharmaceuticals and vaccination technology as well as recombinant DNA technology (Cloning and expression of streptokinase gene & Purification of different biological compounds)

My research project (Dissertation) focused on nanoparticle synthesis (MgO) and estimation of its antimicrobial activity

Attended a workshop on the basics of cell culture techniques B.Sc. Microbiology (From Sardar Patel University) Anand, Gujarat

I gained basic knowledge of microbiology, microbial biotechnology and its application in various field. LANGUAGES

EDUCATION

SKILLS

English, Hindi, Gujarati

Reference: Dr. Debayan Baidya

*************.**@********.**.**, +91-953*******

Lab Development and Management

Documentation

Team work and Quick Learner

Bio-informatics

GLP

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