Molecular Biology Data Management

SHAMIK Das

Birmingham, AL ***** 205-***-**** **********@*****.***

Professional Summary

Determined and well-rounded individual with more than 20 years in Research including 2 years working as Scientist within Pharma-Biotech industry. Sharp and focused professional offering skills in Molecular Biology and mice work. Communicative Scientist proficient in Data Management, preparation and Technical Review. Educated in safety procedures, processes and regulations. Pursuing role and leveraging exceptional technical knowledge to better satisfy customer needs. Knowledgeable in Jump software and statistical approaches. Highly motivated with outstanding writing and interpersonal skills. Proficient in conducting laboratory and field experiments while informing new hires on research methods and data collection procedures. Hardworking and driven with superior organizational and analytical skills combined with unparalleled dedication to research excellence. Skilled in conceptualization, design and implementation of multiple projects. Elaborate experience in laboratory research. Expertise in data analysis and background in molecular biology and genetics. Proficient in advanced laboratory equipment use and techniques. Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals. Hardworking and passionate job seeker with strong organizational skills eager to secure the Scientist position. Ready to help team achieve company goals. Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy. To seek and maintain full-time position that offers professional challenges utilizing interpersonal skills, excellent time management and problem-solving skills.

Skills

Cell & Tissue culture:

Experience in handling different cell lines including both plant and animal cells including over 20 different carcinoma cell lines (commonly MDAMB 231, MDAMB 435, MDAMB 468, SUM 159, SUM 1315, BT 20, MCF 7, MCF 10 AT, MCF 10 CA clone D, T47D etc.; mouse line MC3T3; rat line RAW 264.7; some ovarian lines OV-90 etc.),

Excellent aseptic skills and broad cell culture bioreactor and biochemistry analytical knowledge and experience

Transfection and viral transduction methods: Transient and Stable Cell Lines (Knock In/Outs).

3D cell culture, Colony Formation, Migration and Invasion Assays.

Bone Biology: Osteoblast and osteoclast differentiation, bone pit assay. Effect of different conditioned media, inhibitors and drugs. Isolation of BMDMs from mouse bone marrow.

Cell biology:

Localization by fluorescence microscopy,

Real-time imaging using confocal microscopy,

Immuno-histochemistry and immunocytochemistry.

Protein purification and biochemistry:

Cytosolic and Nuclear Extraction,

Western Blot analysis, Standardization of co-immunoprecipitation methods,

Ligand assays,

ELISAs, FACS, Capillary Electrophoresis.

Molecular Biology:

Isolation of RNA/DNA from cells (also AutoGenprep965 high throughput DNA Extraction),

Cloning, PCR, RT-PCR, qPCR,

In vitro Transcription and Translation,

Electroporation, Transfections (cDNA, promoters, siRNA),

Luciferase assays,

ChIP assays (mammalian cell lines, primary cells), ChIP qPCR,

Use of inhibitors of signaling pathways for target validation, Testing Cancer Drugs,

Cell viability (MTT), proliferation (MTS) and apoptosis (Tunel) assays.

Carbohydrate Biology:

Measuring Glucose consumption and Lactate production. Measuring Glycolysis and cellular metabolism utilizing seahorse,

Various aspects of O-Glcnacylation in cancer cells.

Mice Work:

Collecting different tissues from mice for isolating protein (followed by western blots),

Collecting blood from heart for serum.

Miscellaneous

Technical writing, laboratory noteboo.k, and data analysis skills. Grant writing.

Experience with Process Validation/Process Characterization at lab. Detail-orientated, excellent trouble-shooting aptitude

Strong time management skills and capability of executing multiple projects simultaneously; capable of response promptly to project objective re-prioritization

Strong interpersonal skills, team-orientated, good written and verbal communication skills.

Work History

Lead Contamination Specialist, Quality Assurance, MICRO 10/2024 – 06/2025

Performing and leading a team of technical staff in quality assurance and environmental monitoring.

GDP and Data Management.

Preparing Trend Reports and SOPs.

Senior Scientist, 02/2022 - 11/2023

Molecular Designs LLC – Birmingham, AL

Simultaneously managed several complex projects, meeting all deadlines.

Prioritized project tasks after discussions with R&D department team.

Documented changes during experiments, noting unexpected issues arising during research activities.

Conducted experiments in a laboratory environment for product development purposes.

Supervised Scientists and Technicians in Production.

Coordinated with Clinical Laboratory and Quality Control.

Prepared Presentations for Technical Review and Product Launch.

Developed SOPs.

Research Associate, 02/2021 - 02/2022

UAB Medicine – Birmingham, AL

Gathered, arranged, and corrected research data to create representative graphs and charts highlighting results for presentations.

Helped team meet regulatory requirements by coordinating documentation and filings.

Distilled large amounts of information and produced reports, spreadsheets and documents outlining key details.

Set up equipment, organized inventory and maintained facilities.

Validated incoming data to check information accuracy and integrity while independently locating and correcting concerns.

Completed exhaustive research into Cancer Biology using databases, physical records and digital resources.

Produced actionable information that improved Colon Cancer.

Research Associate, 02/2019 - 01/2021

OUHSC – Oklahoma City, OK

Conduct Research on Pancreatic Cancer and Analyse Data.

Write Papers and prepare presentations.

Researcher V, 02/2013 - 08/2018

UAB – Birmingham, AL

Gathered and organized information for research purposes.

Performed research into study topics to increase knowledge and to provide valuable contributions.

Interpreted data analysis results to draw inferences and conclusions.

Wrote research papers, reports, reviews, and summaries regarding Breast Cancer and Melanoma.

Education

Ph.D.: Life Sciences, 09/2002

Bose Institute - Kolkata

Additional Information

Invention Disclosures and Patents

Patent number: US Patent 9,540,647

Assignee: University of South Alabama

Inventors: Lalita Samant, Rajeev Samant, Shamik Das, Eddie Reed

List of PUBLICATIONS

1.Huai Y, Zhang Y, Xiong X, Das S, Bhattacharya R and Mukherjee P. Gold Nanoparticles sensitize pancreatic cancer cells to gemcitabine. Cell Stress 2019.

2.Das S, Bailey S, Metge B, Hanna A, Hinshaw D, Mota M, Forero-Torres A, Chatham J, Samant R, and Shevde L. O-GlcNAcylation of GLI Transcription Factors in Hyperglycemic Conditions Augments Hedgehog activity" Lab Investigation 2018.

3.Wallis D, Kairong KL, Hui Lui H, Hu K, Chen M, Li, Kang J, Das S, Korf BR, Kesterson RA. Neurofibromin (NF1) Genetic Variant Structure-Function Analyses Using a Full-Length Mouse cDNA. Human Mutation 2018

4.Das S, Jackson WP, Prasain JK, Hanna A, Bailey SK, Tucker JA, Bae S, Wilson LS, Samant RS, Barnes S, Shevde LS. Loss of Merlin induces metabolomic adaptation that engages dependence on Hedgehog signaling. Scientific Reports Scientific Reports Vol 7, Article number: 40773 (2017).

5.Das S, Morrow KA, Kyle Meng E, Menezes ME, Bailey SK, Metge BJ, Buchsbaum DJ, Rajeev Samant RS, Shevde LA. Loss of tumor suppressor Merlin results in aberrant activation of Wnt/β-catenin signaling in cancer. Oncotarget Vol. 7, No.14. doi: 10.18632/oncotarget.7494.

6.Devine DJ, Rostas JW, Metge BJ, Das S, Mulekar MS, Tucker JA, Grizzle WE, Buchsbaum DJ, Shevde LA, Samant RS. Loss of N-Myc interactor promotes epithelial-mesenchymal-transition by activation of TGF-β/SMAD signaling. Oncogene, (17 June 2013) doi:10.1038/onc.2013.215.

7.Das S, Samant RS, Shevde LA. Non-Classical Activation of Hedgehog Signaling Enhances Multidrug Resistance and Makes Cancer Cells Refractory to SMOH-Targeting Hedgehog Inhibition. J Biol Chem. 2013 Apr 26; 288(17):11824-33. doi: 10.1074/jbc.M112.432302. Epub 2013 Mar 18.

8.Das S, Tucker JA, Khullar S, Samant RS, Shevde LA. Hedgehog signaling in tumor cells facilitates osteoblast-enhanced osteolytic metastases. PLoS One. 2012; 7(3):e34374. Epub 2012 Mar 29.

9.Kudo K, Gavin E, Das S, Amable L, Shevde LA, Reed E. Inhibition of Gli1 results in altered c-Jun activation, inhibition of cisplatin-induced upregulation of ERCC1, XPD and XRCC1, and inhibition of platinum-DNA adduct repair. Oncogene. 2012 Jan 23. doi: 10.1038/onc.2011.610.

10.Das S, Samant RS, Shevde LA. The hedgehog pathway conditions the bone microenvironment for osteolytic metastasis of breast cancer. Int J Breast Cancer. 2012; 2012: 298623. Epub 2011 Dec 1.

11.Das S, Morrow KA, Metge BJ, Ye K, Mulekar MS, Tucker JA, Samant RS, Shevde LA. Loss of tumor suppressor Merlin in advanced breast cancer is due to post-translational regulation. J Biol Chem. 2011 Nov 18; 286(46):40376-85. Epub 2011 Sep 30.

12.Saswati Sengupta, S Das, Suparna Ghosh, Amita Pal. Phenolic Signals and Their Perception Leads to in Vitro Shoot Induction in Vigna radiata Cotyledonary Explants (pp 37-41). International Journal of Plant Developmental Biology. Volume 5 Number 1 2011.

13.Das S, Samant RS, Shevde LA. Hedgehog signaling induced by breast cancer cells promotes osteoclastogenesis and osteolysis. J Biol Chem. 2011 Mar 18; 286(11):9612-22. Epub 2010 Dec 18.

14.Shevde L.A., Clark D.W., Das S., Samant R.S. Osteopontin: An effector and an effect of tumor metastasis. Review Article. Curr Mol Med. 2010 Feb 1;10 (1):71-81

15.S Das, Lilliane G Harris, Brandon J Metge, Suhu Liu, Adam I. Riker, Rajeev S. Samant and Lalita A. Shevde. The Hedgehog Pathway Transcription Factor, GLI1 Promotes Malignant Behavior of Cancer Cells by Upregulating Osteopontin. J Biol Chem. 2009 Aug 21;284(34):22888-97. Epub 2009 Jun 25.

16.T Sen, S Moulik, A Dutta, PR Choudhury, A Banerji, S Das, M Roy, A Chatterjee. Multi-functional effect of epigallocatechin-3-gallate (EGCG) in downregulation of gelatinase-A (MMP-2) in human breast cancer cell line MCF-7. Life Sciences 84 (7), 194-204, 2009.

17.Anindita Dutta, Triparna Sen, Aniruddha Banerjee, Shamik Das and Amitava Chatterjee. "Studies on Multifunctional Effect of All-Trans Retinoic Acid (ATRA) on Matrix

Metalloproteinase-2 (MMP-2) and its Regulatory Molecules in Human Breast Cancer Cells (MCF-7)."Journal of Oncology Volume 2009 (2009), Article ID 627840.

18.Moulik S, Sen T, DuttaA, Banerji A, Ghosh, Das S, and Chatterjee A. Phosphatidylinositol 3-Kinase and NF-kB Involved in Epidermal Growth Factor-Induced Matrix Metalloproteinase-9 Expression J. Cancer Mol. 4: 55-60, 2008

19.S Das, A. Banerji, E. Frei, A. Chatterjee. Rapid expression and activation of MMP-2 and MMP-9 upon exposure of human breast cancer cells (MCF-7) to fibronectin in serum free medium. Life Sciences (2008) 467 – 476.

20.A Banerji, S. Das, A. Chatterjee. Culture of human melanoma cells, A375 in presence of fibronectin causes expression of MMP-9 and activation of MMP-2 in culture supernatants. J Environ Pathol Toxicol Oncol. (Vol 27, 2008).

21.Das S, Sarkar C, Chakraborty, Chowdhury UR, Basu B, Dasgupta PS and Basu S. Cutting Edge: Stimulation of Dopamine D4 Receptors Induce T Cell Quiescence by Up-regulating Kruppel-Like Factor-2 Expression through Inhibition of ERK1/ERK2 Phosphorylation. The Journal of Immunology, 2006, 177:7525 – 7529.

A.Mitra, J. Chakrabarti, A. Banerji, S. Das, A. Chatterjee. Culture of human cervical cancer cells, SiHa in presence of fibronectin activates MMP-2. J Cancer Res Clin Oncol 132 (2006) 505 - 513.

A.Mitra, J. Chakrabarti, A. Banerji, S. Das, A. Chatterjee. Tumour cell surface integrins and matrix metalloproteinases cross-talk: a potential area for developing anti-metastatic drugs. Current trends in Pharmacology, ed. Roy and Gulati (2007) Chapter 26, 437-441.

22.Das S, Sengupta DN and Pal A. The differential protein patterns of two cotyledon explants of Vigna radiata during induced in vitro differentiation: Probable implication in the conundrum of differential regeneration response. (J. Plant Biochem. & Biotech, Vol15, 123 – 129, July 2006).

23.Das S and Pal A (2004) Differential regeneration response in two cotyledons of Vigna radiata: Histomorphological analysis and effect of -arabinogalactan. J. Plant Biochem. & Biotech. 13, 101 – 106, July 2004.

24.Das S and Pal A (2003) Differential DNA endoreduplication and protein profile during cotyledon ontogeny of Vigna radiata. J. Plant Biochem. & Biotech. 12: 11-18, January 2003.

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